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Expression of microRNA‐like RNA‐2 (Fgmil‐2) and bioH1 from a single transcript in Fusarium graminearum are inversely correlated to regulate biotin synthesis during vegetative growth and host infection

MicroRNA‐like RNAs (milRNAs) post‐transcriptionally down‐regulate target genes. We investigated Fusarium graminearum (Fg) milRNA expression during fungal vegetative growth and infection of wheat. Small RNA sequencing identified 36 milRNAs from Fg, one of which, Fgmil‐2, had >100 transcripts per m...

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Autores principales: Guo, Mao‐Wei, Yang, Peng, Zhang, Jing‐Bo, Liu, Gang, Yuan, Qing‐Song, He, Wei‐Jie, Nian, Jun‐Na, Yi, Shu‐Yuan, Huang, Tao, Liao, Yu‐Cai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804420/
https://www.ncbi.nlm.nih.gov/pubmed/31385410
http://dx.doi.org/10.1111/mpp.12859
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author Guo, Mao‐Wei
Yang, Peng
Zhang, Jing‐Bo
Liu, Gang
Yuan, Qing‐Song
He, Wei‐Jie
Nian, Jun‐Na
Yi, Shu‐Yuan
Huang, Tao
Liao, Yu‐Cai
author_facet Guo, Mao‐Wei
Yang, Peng
Zhang, Jing‐Bo
Liu, Gang
Yuan, Qing‐Song
He, Wei‐Jie
Nian, Jun‐Na
Yi, Shu‐Yuan
Huang, Tao
Liao, Yu‐Cai
author_sort Guo, Mao‐Wei
collection PubMed
description MicroRNA‐like RNAs (milRNAs) post‐transcriptionally down‐regulate target genes. We investigated Fusarium graminearum (Fg) milRNA expression during fungal vegetative growth and infection of wheat. Small RNA sequencing identified 36 milRNAs from Fg, one of which, Fgmil‐2, had >100 transcripts per million in conidia, mycelia and infected wheat, with the highest expression in conidia and the lowest expression in colonized wheat tissue. Fgmil‐2 displays perfect homology to the 3ʹ‐untranslated region (3ʹ‐UTR) of an FgbioH1 messenger RNA that is involved in biotin biosynthesis. Poly(A) polymerase‐mediated rapid amplification of cDNA ends combined with sequencing analysis demonstrated that cleavage at a specific site by FgDicer2 in the 3ʹ‐UTR of FgbioH1 transcripts generated the Fgmil‐2 precursor with a typical hairpin structure. Deletion of FgbioH1 or FgDicer2 genes abolished Fgmil‐2 biogenesis. FgbioH1 had an inversely correlated pattern of expression to that of Fgmil‐2 and FgDicer2. Deletion of FgbioH1 also showed that it is required for mycelial growth, virulence, mycotoxin biosynthesis and expression of biotin‐dependent carboxylase genes. This study reveals in Fg a novel mode of inversely correlated post‐transcriptional regulation in which Fgmil‐2 originates from its own target transcript, FgbioH, to govern biotin biosynthesis.
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spelling pubmed-68044202019-10-24 Expression of microRNA‐like RNA‐2 (Fgmil‐2) and bioH1 from a single transcript in Fusarium graminearum are inversely correlated to regulate biotin synthesis during vegetative growth and host infection Guo, Mao‐Wei Yang, Peng Zhang, Jing‐Bo Liu, Gang Yuan, Qing‐Song He, Wei‐Jie Nian, Jun‐Na Yi, Shu‐Yuan Huang, Tao Liao, Yu‐Cai Mol Plant Pathol Short Communications MicroRNA‐like RNAs (milRNAs) post‐transcriptionally down‐regulate target genes. We investigated Fusarium graminearum (Fg) milRNA expression during fungal vegetative growth and infection of wheat. Small RNA sequencing identified 36 milRNAs from Fg, one of which, Fgmil‐2, had >100 transcripts per million in conidia, mycelia and infected wheat, with the highest expression in conidia and the lowest expression in colonized wheat tissue. Fgmil‐2 displays perfect homology to the 3ʹ‐untranslated region (3ʹ‐UTR) of an FgbioH1 messenger RNA that is involved in biotin biosynthesis. Poly(A) polymerase‐mediated rapid amplification of cDNA ends combined with sequencing analysis demonstrated that cleavage at a specific site by FgDicer2 in the 3ʹ‐UTR of FgbioH1 transcripts generated the Fgmil‐2 precursor with a typical hairpin structure. Deletion of FgbioH1 or FgDicer2 genes abolished Fgmil‐2 biogenesis. FgbioH1 had an inversely correlated pattern of expression to that of Fgmil‐2 and FgDicer2. Deletion of FgbioH1 also showed that it is required for mycelial growth, virulence, mycotoxin biosynthesis and expression of biotin‐dependent carboxylase genes. This study reveals in Fg a novel mode of inversely correlated post‐transcriptional regulation in which Fgmil‐2 originates from its own target transcript, FgbioH, to govern biotin biosynthesis. John Wiley and Sons Inc. 2019-08-06 /pmc/articles/PMC6804420/ /pubmed/31385410 http://dx.doi.org/10.1111/mpp.12859 Text en © 2019 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communications
Guo, Mao‐Wei
Yang, Peng
Zhang, Jing‐Bo
Liu, Gang
Yuan, Qing‐Song
He, Wei‐Jie
Nian, Jun‐Na
Yi, Shu‐Yuan
Huang, Tao
Liao, Yu‐Cai
Expression of microRNA‐like RNA‐2 (Fgmil‐2) and bioH1 from a single transcript in Fusarium graminearum are inversely correlated to regulate biotin synthesis during vegetative growth and host infection
title Expression of microRNA‐like RNA‐2 (Fgmil‐2) and bioH1 from a single transcript in Fusarium graminearum are inversely correlated to regulate biotin synthesis during vegetative growth and host infection
title_full Expression of microRNA‐like RNA‐2 (Fgmil‐2) and bioH1 from a single transcript in Fusarium graminearum are inversely correlated to regulate biotin synthesis during vegetative growth and host infection
title_fullStr Expression of microRNA‐like RNA‐2 (Fgmil‐2) and bioH1 from a single transcript in Fusarium graminearum are inversely correlated to regulate biotin synthesis during vegetative growth and host infection
title_full_unstemmed Expression of microRNA‐like RNA‐2 (Fgmil‐2) and bioH1 from a single transcript in Fusarium graminearum are inversely correlated to regulate biotin synthesis during vegetative growth and host infection
title_short Expression of microRNA‐like RNA‐2 (Fgmil‐2) and bioH1 from a single transcript in Fusarium graminearum are inversely correlated to regulate biotin synthesis during vegetative growth and host infection
title_sort expression of microrna‐like rna‐2 (fgmil‐2) and bioh1 from a single transcript in fusarium graminearum are inversely correlated to regulate biotin synthesis during vegetative growth and host infection
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804420/
https://www.ncbi.nlm.nih.gov/pubmed/31385410
http://dx.doi.org/10.1111/mpp.12859
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