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Auxin response factor 6A regulates photosynthesis, sugar accumulation, and fruit development in tomato
Auxin response factors (ARFs) are involved in auxin-mediated transcriptional regulation in plants. In this study, we performed functional characterization of SlARF6A in tomato. SlARF6A is located in the nucleus and exhibits transcriptional activator activity. Overexpression of SlARF6A increased chlo...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804849/ https://www.ncbi.nlm.nih.gov/pubmed/31645946 http://dx.doi.org/10.1038/s41438-019-0167-x |
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author | Yuan, Yujin Xu, Xin Gong, Zehao Tang, Yuwei Wu, Mengbo Yan, Fang Zhang, Xiaolan Zhang, Qian Yang, Fengqing Hu, Xiaowei Yang, Qichen Luo, Yingqing Mei, Lihua Zhang, Wenfa Jiang, Cai-Zhong Lu, Wangjin Li, Zhengguo Deng, Wei |
author_facet | Yuan, Yujin Xu, Xin Gong, Zehao Tang, Yuwei Wu, Mengbo Yan, Fang Zhang, Xiaolan Zhang, Qian Yang, Fengqing Hu, Xiaowei Yang, Qichen Luo, Yingqing Mei, Lihua Zhang, Wenfa Jiang, Cai-Zhong Lu, Wangjin Li, Zhengguo Deng, Wei |
author_sort | Yuan, Yujin |
collection | PubMed |
description | Auxin response factors (ARFs) are involved in auxin-mediated transcriptional regulation in plants. In this study, we performed functional characterization of SlARF6A in tomato. SlARF6A is located in the nucleus and exhibits transcriptional activator activity. Overexpression of SlARF6A increased chlorophyll contents in the fruits and leaves of tomato plants, whereas downregulation of SlARF6A decreased chlorophyll contents compared with those of wild-type (WT) plants. Analysis of chloroplasts using transmission electron microscopy indicated increased sizes of chloroplasts in SlARF6A-overexpressing plants and decreased numbers of chloroplasts in SlARF6A-downregulated plants. Overexpression of SlARF6A increased the photosynthesis rate and accumulation of starch and soluble sugars, whereas knockdown of SlARF6A resulted in opposite phenotypes in tomato leaves and fruits. RNA-sequence analysis showed that regulation of SlARF6A expression altered the expression of genes involved in chlorophyll metabolism, photosynthesis and sugar metabolism. SlARF6A directly bound to the promoters of SlGLK1, CAB, and RbcS genes and positively regulated the expression of these genes. Overexpression of SlARF6A also inhibited fruit ripening and ethylene production, whereas downregulation of SlARF6A increased fruit ripening and ethylene production. SlARF6A directly bound to the SAMS1 promoter and negatively regulated SAMS1 expression. Taken together, these results expand our understanding of ARFs with regard to photosynthesis, sugar accumulation and fruit development and provide a potential target for genetic engineering to improve fruit nutrition in horticulture crops. |
format | Online Article Text |
id | pubmed-6804849 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-68048492019-10-23 Auxin response factor 6A regulates photosynthesis, sugar accumulation, and fruit development in tomato Yuan, Yujin Xu, Xin Gong, Zehao Tang, Yuwei Wu, Mengbo Yan, Fang Zhang, Xiaolan Zhang, Qian Yang, Fengqing Hu, Xiaowei Yang, Qichen Luo, Yingqing Mei, Lihua Zhang, Wenfa Jiang, Cai-Zhong Lu, Wangjin Li, Zhengguo Deng, Wei Hortic Res Article Auxin response factors (ARFs) are involved in auxin-mediated transcriptional regulation in plants. In this study, we performed functional characterization of SlARF6A in tomato. SlARF6A is located in the nucleus and exhibits transcriptional activator activity. Overexpression of SlARF6A increased chlorophyll contents in the fruits and leaves of tomato plants, whereas downregulation of SlARF6A decreased chlorophyll contents compared with those of wild-type (WT) plants. Analysis of chloroplasts using transmission electron microscopy indicated increased sizes of chloroplasts in SlARF6A-overexpressing plants and decreased numbers of chloroplasts in SlARF6A-downregulated plants. Overexpression of SlARF6A increased the photosynthesis rate and accumulation of starch and soluble sugars, whereas knockdown of SlARF6A resulted in opposite phenotypes in tomato leaves and fruits. RNA-sequence analysis showed that regulation of SlARF6A expression altered the expression of genes involved in chlorophyll metabolism, photosynthesis and sugar metabolism. SlARF6A directly bound to the promoters of SlGLK1, CAB, and RbcS genes and positively regulated the expression of these genes. Overexpression of SlARF6A also inhibited fruit ripening and ethylene production, whereas downregulation of SlARF6A increased fruit ripening and ethylene production. SlARF6A directly bound to the SAMS1 promoter and negatively regulated SAMS1 expression. Taken together, these results expand our understanding of ARFs with regard to photosynthesis, sugar accumulation and fruit development and provide a potential target for genetic engineering to improve fruit nutrition in horticulture crops. Nature Publishing Group UK 2019-07-11 /pmc/articles/PMC6804849/ /pubmed/31645946 http://dx.doi.org/10.1038/s41438-019-0167-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Yuan, Yujin Xu, Xin Gong, Zehao Tang, Yuwei Wu, Mengbo Yan, Fang Zhang, Xiaolan Zhang, Qian Yang, Fengqing Hu, Xiaowei Yang, Qichen Luo, Yingqing Mei, Lihua Zhang, Wenfa Jiang, Cai-Zhong Lu, Wangjin Li, Zhengguo Deng, Wei Auxin response factor 6A regulates photosynthesis, sugar accumulation, and fruit development in tomato |
title | Auxin response factor 6A regulates photosynthesis, sugar accumulation, and fruit development in tomato |
title_full | Auxin response factor 6A regulates photosynthesis, sugar accumulation, and fruit development in tomato |
title_fullStr | Auxin response factor 6A regulates photosynthesis, sugar accumulation, and fruit development in tomato |
title_full_unstemmed | Auxin response factor 6A regulates photosynthesis, sugar accumulation, and fruit development in tomato |
title_short | Auxin response factor 6A regulates photosynthesis, sugar accumulation, and fruit development in tomato |
title_sort | auxin response factor 6a regulates photosynthesis, sugar accumulation, and fruit development in tomato |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804849/ https://www.ncbi.nlm.nih.gov/pubmed/31645946 http://dx.doi.org/10.1038/s41438-019-0167-x |
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