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Cyathea gigantea (Cyatheaceae) as an antimicrobial agent against multidrug resistant organisms

BACKGROUND: Rapid emergence of multidrug resistant (MDR) organisms in hospital and community settings often result into treatment failure, thus leading the clinicians with fewer treatment options. Cyathea gigantea, an ethnomedicinally important fern used in cuts and wound infections. So, if this med...

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Autores principales: Nath, Kathakali, Talukdar, Anupam Das, Bhattacharya, Mrinal Kanti, Bhowmik, Deepshikha, Chetri, Shiela, Choudhury, Debarati, Mitra, Abhijit, Choudhury, Nargis Alom
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6805519/
https://www.ncbi.nlm.nih.gov/pubmed/31640666
http://dx.doi.org/10.1186/s12906-019-2696-0
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author Nath, Kathakali
Talukdar, Anupam Das
Bhattacharya, Mrinal Kanti
Bhowmik, Deepshikha
Chetri, Shiela
Choudhury, Debarati
Mitra, Abhijit
Choudhury, Nargis Alom
author_facet Nath, Kathakali
Talukdar, Anupam Das
Bhattacharya, Mrinal Kanti
Bhowmik, Deepshikha
Chetri, Shiela
Choudhury, Debarati
Mitra, Abhijit
Choudhury, Nargis Alom
author_sort Nath, Kathakali
collection PubMed
description BACKGROUND: Rapid emergence of multidrug resistant (MDR) organisms in hospital and community settings often result into treatment failure, thus leading the clinicians with fewer treatment options. Cyathea gigantea, an ethnomedicinally important fern used in cuts and wound infections. So, if this medicinal plant is used in treating the MDR infections then it might bring certain relief in future treatment options. METHODS: Antibacterial activity of C. gigantea against MDR bacteria was assed using well diffusion and broth microdilution methods to determine the diameters of growth inhibition zones, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Synergistic activity was also determined with the conventional antibiotics by disc diffusion method followed by FIC index of each of the tested antibiotic was calculated. The active extract was then subjected to fractionation by column chromatography and antibacterial activity was done with each of the collected fractions. RESULTS: Crude extract of C. gigantea was found to be active against all the tested organisms. The MIC was 200 μg/ml against Gram-positive i.e., Staphylococcus aureus ATCC 25923 and 400 μg/ml against Gram-negative i.e., Escherichia coli ATCC 25922 and Pseudomonas aeruginosa PAO1, while the MBC was 400 μg/ml in case of Gram-positive and 800 μg/ml for Gram-negative. The synergistic activity revealed that the plant extract increased the antibacterial property of the studied antibiotics and the FIC index showed that significant synergistic activity was shown by ciprofloxacin followed by tetracycline, ampicillin and oxacillin. Antibacterial activity with the fractionated extract showed that the FR II, FR III and FR IV were active against both Gram-positive and Gram-negative bacteria, whereas FR I, FR V and FR VI did not show antibacterial property against any of the tested bacteria. CONCLUSIONS: Extracts of C. gigantea was found active against both selected Gram-positive and Gram-negative organisms and thus offers the scientific basis for the traditional use of the fern. The present study also provides the basis for future study to validate the possible use against multidrug resistant organisms.
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spelling pubmed-68055192019-10-24 Cyathea gigantea (Cyatheaceae) as an antimicrobial agent against multidrug resistant organisms Nath, Kathakali Talukdar, Anupam Das Bhattacharya, Mrinal Kanti Bhowmik, Deepshikha Chetri, Shiela Choudhury, Debarati Mitra, Abhijit Choudhury, Nargis Alom BMC Complement Altern Med Research Article BACKGROUND: Rapid emergence of multidrug resistant (MDR) organisms in hospital and community settings often result into treatment failure, thus leading the clinicians with fewer treatment options. Cyathea gigantea, an ethnomedicinally important fern used in cuts and wound infections. So, if this medicinal plant is used in treating the MDR infections then it might bring certain relief in future treatment options. METHODS: Antibacterial activity of C. gigantea against MDR bacteria was assed using well diffusion and broth microdilution methods to determine the diameters of growth inhibition zones, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Synergistic activity was also determined with the conventional antibiotics by disc diffusion method followed by FIC index of each of the tested antibiotic was calculated. The active extract was then subjected to fractionation by column chromatography and antibacterial activity was done with each of the collected fractions. RESULTS: Crude extract of C. gigantea was found to be active against all the tested organisms. The MIC was 200 μg/ml against Gram-positive i.e., Staphylococcus aureus ATCC 25923 and 400 μg/ml against Gram-negative i.e., Escherichia coli ATCC 25922 and Pseudomonas aeruginosa PAO1, while the MBC was 400 μg/ml in case of Gram-positive and 800 μg/ml for Gram-negative. The synergistic activity revealed that the plant extract increased the antibacterial property of the studied antibiotics and the FIC index showed that significant synergistic activity was shown by ciprofloxacin followed by tetracycline, ampicillin and oxacillin. Antibacterial activity with the fractionated extract showed that the FR II, FR III and FR IV were active against both Gram-positive and Gram-negative bacteria, whereas FR I, FR V and FR VI did not show antibacterial property against any of the tested bacteria. CONCLUSIONS: Extracts of C. gigantea was found active against both selected Gram-positive and Gram-negative organisms and thus offers the scientific basis for the traditional use of the fern. The present study also provides the basis for future study to validate the possible use against multidrug resistant organisms. BioMed Central 2019-10-22 /pmc/articles/PMC6805519/ /pubmed/31640666 http://dx.doi.org/10.1186/s12906-019-2696-0 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Nath, Kathakali
Talukdar, Anupam Das
Bhattacharya, Mrinal Kanti
Bhowmik, Deepshikha
Chetri, Shiela
Choudhury, Debarati
Mitra, Abhijit
Choudhury, Nargis Alom
Cyathea gigantea (Cyatheaceae) as an antimicrobial agent against multidrug resistant organisms
title Cyathea gigantea (Cyatheaceae) as an antimicrobial agent against multidrug resistant organisms
title_full Cyathea gigantea (Cyatheaceae) as an antimicrobial agent against multidrug resistant organisms
title_fullStr Cyathea gigantea (Cyatheaceae) as an antimicrobial agent against multidrug resistant organisms
title_full_unstemmed Cyathea gigantea (Cyatheaceae) as an antimicrobial agent against multidrug resistant organisms
title_short Cyathea gigantea (Cyatheaceae) as an antimicrobial agent against multidrug resistant organisms
title_sort cyathea gigantea (cyatheaceae) as an antimicrobial agent against multidrug resistant organisms
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6805519/
https://www.ncbi.nlm.nih.gov/pubmed/31640666
http://dx.doi.org/10.1186/s12906-019-2696-0
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