Cargando…

Molecular investigation and phylogeny of species of the Anaplasmataceae infecting animals and ticks in Senegal

BACKGROUND: Our study aimed to assess the diversity of the species of Anaplasmataceae in Senegal that infect animals and ticks in three areas: near Keur Momar Sarr (northern region), Dielmo and Diop (Sine Saloum, central region of Senegal), and in Casamance (southern region of Senegal). METHODS: A t...

Descripción completa

Detalles Bibliográficos
Autores principales: Dahmani, Mustapha, Davoust, Bernard, Sambou, Masse, Bassene, Hubert, Scandola, Pierre, Ameur, Tinhinene, Raoult, Didier, Fenollar, Florence, Mediannikov, Oleg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6805679/
https://www.ncbi.nlm.nih.gov/pubmed/31640746
http://dx.doi.org/10.1186/s13071-019-3742-y
_version_ 1783461448251342848
author Dahmani, Mustapha
Davoust, Bernard
Sambou, Masse
Bassene, Hubert
Scandola, Pierre
Ameur, Tinhinene
Raoult, Didier
Fenollar, Florence
Mediannikov, Oleg
author_facet Dahmani, Mustapha
Davoust, Bernard
Sambou, Masse
Bassene, Hubert
Scandola, Pierre
Ameur, Tinhinene
Raoult, Didier
Fenollar, Florence
Mediannikov, Oleg
author_sort Dahmani, Mustapha
collection PubMed
description BACKGROUND: Our study aimed to assess the diversity of the species of Anaplasmataceae in Senegal that infect animals and ticks in three areas: near Keur Momar Sarr (northern region), Dielmo and Diop (Sine Saloum, central region of Senegal), and in Casamance (southern region of Senegal). METHODS: A total of 204 ticks and 433 blood samples were collected from ruminants, horses, donkeys and dogs. Ticks were identified morphologically and by molecular characterization targeting the 12S rRNA gene. Molecular characterization of species of Anaplasmataceae infecting Senegalese ticks and animals was conducted using the 23S rRNA, 16S rRNA, rpoB and groEL genes. RESULTS: Ticks were identified as Rhipicephalus evertsi evertsi (84.3%), Hyalomma rufipes (8.3%), Hyalomma impeltatum (4.9%), R. bursa (1.5%) and R. muhsamae (0.9%). The overall prevalence of Anaplasmataceae infection in ticks was 0.9%, whereas 41.1% of the sampled animals were found infected by one of the species belonging to this family. We identified the pathogen Anaplasma ovis in 55.9% of sheep, A. marginale and A. centrale in 19.4% and 8.1%, respectively, of cattle, as well as a putative new species of Anaplasmataceae. Two Anaplasma species commonly infecting ruminants were identified. Anaplasma cf. platys, closely related to A. platys was identified in 19.8% of sheep, 27.7% of goats and 22.6% of cattle, whereas a putative new species, named here provisionally “Candidatus Anaplasma africae”, was identified in 3.7% of sheep, 10.3% of goats and 8.1% of cattle. Ehrlichia canis and Anaplasma platys were identified only from dogs sampled in the Keur Momar Sarr area. Ehrlichia canis was identified in 18.8% of dogs and two R. e. evertsi ticks removed from the same sheep. Anaplasma platys was identified in 15.6% of dogs. Neither of the dogs sampled from Casamance region nor the horses and donkeys sampled from Keur Momar Sarr area were found infected by an Anaplasmataceae species. CONCLUSIONS: This study presents a summary of Anaplasmataceae species that infect animals and ticks in three areas from the northern, central and southern regions of Senegal. To our knowledge, our findings demonstrate for the first time the presence of multiple Anaplasmataceae species that infect ticks and domestic animals in Senegal. We recorded two potentially new species commonly infecting ruminants named here provisionally as Anaplasma cf. platys and “Candidatus Anaplasma africae”. However, E. canis was the only species identified and amplified from ticks. None of the other Anaplasmataceae species identified in animals were identified in the tick species collected from animals.
format Online
Article
Text
id pubmed-6805679
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-68056792019-10-24 Molecular investigation and phylogeny of species of the Anaplasmataceae infecting animals and ticks in Senegal Dahmani, Mustapha Davoust, Bernard Sambou, Masse Bassene, Hubert Scandola, Pierre Ameur, Tinhinene Raoult, Didier Fenollar, Florence Mediannikov, Oleg Parasit Vectors Research BACKGROUND: Our study aimed to assess the diversity of the species of Anaplasmataceae in Senegal that infect animals and ticks in three areas: near Keur Momar Sarr (northern region), Dielmo and Diop (Sine Saloum, central region of Senegal), and in Casamance (southern region of Senegal). METHODS: A total of 204 ticks and 433 blood samples were collected from ruminants, horses, donkeys and dogs. Ticks were identified morphologically and by molecular characterization targeting the 12S rRNA gene. Molecular characterization of species of Anaplasmataceae infecting Senegalese ticks and animals was conducted using the 23S rRNA, 16S rRNA, rpoB and groEL genes. RESULTS: Ticks were identified as Rhipicephalus evertsi evertsi (84.3%), Hyalomma rufipes (8.3%), Hyalomma impeltatum (4.9%), R. bursa (1.5%) and R. muhsamae (0.9%). The overall prevalence of Anaplasmataceae infection in ticks was 0.9%, whereas 41.1% of the sampled animals were found infected by one of the species belonging to this family. We identified the pathogen Anaplasma ovis in 55.9% of sheep, A. marginale and A. centrale in 19.4% and 8.1%, respectively, of cattle, as well as a putative new species of Anaplasmataceae. Two Anaplasma species commonly infecting ruminants were identified. Anaplasma cf. platys, closely related to A. platys was identified in 19.8% of sheep, 27.7% of goats and 22.6% of cattle, whereas a putative new species, named here provisionally “Candidatus Anaplasma africae”, was identified in 3.7% of sheep, 10.3% of goats and 8.1% of cattle. Ehrlichia canis and Anaplasma platys were identified only from dogs sampled in the Keur Momar Sarr area. Ehrlichia canis was identified in 18.8% of dogs and two R. e. evertsi ticks removed from the same sheep. Anaplasma platys was identified in 15.6% of dogs. Neither of the dogs sampled from Casamance region nor the horses and donkeys sampled from Keur Momar Sarr area were found infected by an Anaplasmataceae species. CONCLUSIONS: This study presents a summary of Anaplasmataceae species that infect animals and ticks in three areas from the northern, central and southern regions of Senegal. To our knowledge, our findings demonstrate for the first time the presence of multiple Anaplasmataceae species that infect ticks and domestic animals in Senegal. We recorded two potentially new species commonly infecting ruminants named here provisionally as Anaplasma cf. platys and “Candidatus Anaplasma africae”. However, E. canis was the only species identified and amplified from ticks. None of the other Anaplasmataceae species identified in animals were identified in the tick species collected from animals. BioMed Central 2019-10-22 /pmc/articles/PMC6805679/ /pubmed/31640746 http://dx.doi.org/10.1186/s13071-019-3742-y Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Dahmani, Mustapha
Davoust, Bernard
Sambou, Masse
Bassene, Hubert
Scandola, Pierre
Ameur, Tinhinene
Raoult, Didier
Fenollar, Florence
Mediannikov, Oleg
Molecular investigation and phylogeny of species of the Anaplasmataceae infecting animals and ticks in Senegal
title Molecular investigation and phylogeny of species of the Anaplasmataceae infecting animals and ticks in Senegal
title_full Molecular investigation and phylogeny of species of the Anaplasmataceae infecting animals and ticks in Senegal
title_fullStr Molecular investigation and phylogeny of species of the Anaplasmataceae infecting animals and ticks in Senegal
title_full_unstemmed Molecular investigation and phylogeny of species of the Anaplasmataceae infecting animals and ticks in Senegal
title_short Molecular investigation and phylogeny of species of the Anaplasmataceae infecting animals and ticks in Senegal
title_sort molecular investigation and phylogeny of species of the anaplasmataceae infecting animals and ticks in senegal
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6805679/
https://www.ncbi.nlm.nih.gov/pubmed/31640746
http://dx.doi.org/10.1186/s13071-019-3742-y
work_keys_str_mv AT dahmanimustapha molecularinvestigationandphylogenyofspeciesoftheanaplasmataceaeinfectinganimalsandticksinsenegal
AT davoustbernard molecularinvestigationandphylogenyofspeciesoftheanaplasmataceaeinfectinganimalsandticksinsenegal
AT samboumasse molecularinvestigationandphylogenyofspeciesoftheanaplasmataceaeinfectinganimalsandticksinsenegal
AT bassenehubert molecularinvestigationandphylogenyofspeciesoftheanaplasmataceaeinfectinganimalsandticksinsenegal
AT scandolapierre molecularinvestigationandphylogenyofspeciesoftheanaplasmataceaeinfectinganimalsandticksinsenegal
AT ameurtinhinene molecularinvestigationandphylogenyofspeciesoftheanaplasmataceaeinfectinganimalsandticksinsenegal
AT raoultdidier molecularinvestigationandphylogenyofspeciesoftheanaplasmataceaeinfectinganimalsandticksinsenegal
AT fenollarflorence molecularinvestigationandphylogenyofspeciesoftheanaplasmataceaeinfectinganimalsandticksinsenegal
AT mediannikovoleg molecularinvestigationandphylogenyofspeciesoftheanaplasmataceaeinfectinganimalsandticksinsenegal