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Comparative transcriptome profiling of immune response against Vibrio harveyi infection in Chinese tongue sole

Vibrio harveyi is a major bacterial pathogen that causes fatal vibriosis in Chinese tongue sole (Cynoglossus semilaevis), resulting in massive mortality in the farming industry. However, the molecular mechanisms of C. semilaevis response to V. harveyi infection are poorly understood. Here, we perfor...

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Autores principales: Xu, Hao, Xu, Xiwen, Li, Xihong, Wang, Lei, Cheng, Jiayu, Zhou, Qian, Chen, Songlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6805913/
https://www.ncbi.nlm.nih.gov/pubmed/31641148
http://dx.doi.org/10.1038/s41597-019-0231-2
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author Xu, Hao
Xu, Xiwen
Li, Xihong
Wang, Lei
Cheng, Jiayu
Zhou, Qian
Chen, Songlin
author_facet Xu, Hao
Xu, Xiwen
Li, Xihong
Wang, Lei
Cheng, Jiayu
Zhou, Qian
Chen, Songlin
author_sort Xu, Hao
collection PubMed
description Vibrio harveyi is a major bacterial pathogen that causes fatal vibriosis in Chinese tongue sole (Cynoglossus semilaevis), resulting in massive mortality in the farming industry. However, the molecular mechanisms of C. semilaevis response to V. harveyi infection are poorly understood. Here, we performed transcriptomic analysis of C. semilaevis, comparing resistant and susceptible families in response to V. harveyi challenge (CsRC and CsSC) and control conditions (CsRU and CsSU). RNA libraries were constructed using 12 RNA samples isolated from three biological replicates of the four groups. We performed transcriptome sequencing on an Illumina HiSeq platform, and generating a total of 1,095 million paired-end reads, with the number of clean reads per library ranging from 75.27 M to 99.97 M. Through pairwise comparisons among the four groups, we identified 713 genes exhibiting significant differences at the transcript level. Furthermore, the expression levels were validated by real-time qPCR. Our results provide a valuable resource and new insights into the immune response to V. harveyi infection.
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spelling pubmed-68059132019-10-30 Comparative transcriptome profiling of immune response against Vibrio harveyi infection in Chinese tongue sole Xu, Hao Xu, Xiwen Li, Xihong Wang, Lei Cheng, Jiayu Zhou, Qian Chen, Songlin Sci Data Data Descriptor Vibrio harveyi is a major bacterial pathogen that causes fatal vibriosis in Chinese tongue sole (Cynoglossus semilaevis), resulting in massive mortality in the farming industry. However, the molecular mechanisms of C. semilaevis response to V. harveyi infection are poorly understood. Here, we performed transcriptomic analysis of C. semilaevis, comparing resistant and susceptible families in response to V. harveyi challenge (CsRC and CsSC) and control conditions (CsRU and CsSU). RNA libraries were constructed using 12 RNA samples isolated from three biological replicates of the four groups. We performed transcriptome sequencing on an Illumina HiSeq platform, and generating a total of 1,095 million paired-end reads, with the number of clean reads per library ranging from 75.27 M to 99.97 M. Through pairwise comparisons among the four groups, we identified 713 genes exhibiting significant differences at the transcript level. Furthermore, the expression levels were validated by real-time qPCR. Our results provide a valuable resource and new insights into the immune response to V. harveyi infection. Nature Publishing Group UK 2019-10-22 /pmc/articles/PMC6805913/ /pubmed/31641148 http://dx.doi.org/10.1038/s41597-019-0231-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadata files associated with this article.
spellingShingle Data Descriptor
Xu, Hao
Xu, Xiwen
Li, Xihong
Wang, Lei
Cheng, Jiayu
Zhou, Qian
Chen, Songlin
Comparative transcriptome profiling of immune response against Vibrio harveyi infection in Chinese tongue sole
title Comparative transcriptome profiling of immune response against Vibrio harveyi infection in Chinese tongue sole
title_full Comparative transcriptome profiling of immune response against Vibrio harveyi infection in Chinese tongue sole
title_fullStr Comparative transcriptome profiling of immune response against Vibrio harveyi infection in Chinese tongue sole
title_full_unstemmed Comparative transcriptome profiling of immune response against Vibrio harveyi infection in Chinese tongue sole
title_short Comparative transcriptome profiling of immune response against Vibrio harveyi infection in Chinese tongue sole
title_sort comparative transcriptome profiling of immune response against vibrio harveyi infection in chinese tongue sole
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6805913/
https://www.ncbi.nlm.nih.gov/pubmed/31641148
http://dx.doi.org/10.1038/s41597-019-0231-2
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