Cargando…
Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging
This paper reports the feasibility of Nakagami imaging in monitoring the regeneration process of zebrafish hearts in a noninvasive manner. In addition, spectral Doppler waveforms that are typically used to access the diastolic function were measured to validate the performance of Nakagami imaging. A...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6806172/ https://www.ncbi.nlm.nih.gov/pubmed/31546705 http://dx.doi.org/10.3390/s19194094 |
_version_ | 1783461567240601600 |
---|---|
author | Yeo, Sunmi Yoon, Changhan Lien, Ching-Ling Song, Tai-Kyong Shung, K. Kirk |
author_facet | Yeo, Sunmi Yoon, Changhan Lien, Ching-Ling Song, Tai-Kyong Shung, K. Kirk |
author_sort | Yeo, Sunmi |
collection | PubMed |
description | This paper reports the feasibility of Nakagami imaging in monitoring the regeneration process of zebrafish hearts in a noninvasive manner. In addition, spectral Doppler waveforms that are typically used to access the diastolic function were measured to validate the performance of Nakagami imaging. A 30-MHz high-frequency ultrasound array transducer was used to acquire backscattered echo signal for spectral Doppler and Nakagami imaging. The performances of both methods were validated with flow and tissue-mimicking phantom experiments. For in vivo experiments, both spectral Doppler and Nakagami imaging were simultaneously obtained from adult zebrafish with amputated hearts. Longitudinal measurements were performed for five zebrafish. From the experiments, the E/A ratio measured using spectral Doppler imaging increased at 3 days post-amputation (3 dpa) and then decreased to the value before amputation, which were consistent with previous studies. Similar results were obtained from the Nakagami imaging where the Nakagami parameter value increased at 3 dpa and decreased to its original value. These results suggested that the Nakagami and spectral Doppler imaging would be useful techniques in monitoring the regeneration of heart or tissues. |
format | Online Article Text |
id | pubmed-6806172 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68061722019-11-07 Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging Yeo, Sunmi Yoon, Changhan Lien, Ching-Ling Song, Tai-Kyong Shung, K. Kirk Sensors (Basel) Article This paper reports the feasibility of Nakagami imaging in monitoring the regeneration process of zebrafish hearts in a noninvasive manner. In addition, spectral Doppler waveforms that are typically used to access the diastolic function were measured to validate the performance of Nakagami imaging. A 30-MHz high-frequency ultrasound array transducer was used to acquire backscattered echo signal for spectral Doppler and Nakagami imaging. The performances of both methods were validated with flow and tissue-mimicking phantom experiments. For in vivo experiments, both spectral Doppler and Nakagami imaging were simultaneously obtained from adult zebrafish with amputated hearts. Longitudinal measurements were performed for five zebrafish. From the experiments, the E/A ratio measured using spectral Doppler imaging increased at 3 days post-amputation (3 dpa) and then decreased to the value before amputation, which were consistent with previous studies. Similar results were obtained from the Nakagami imaging where the Nakagami parameter value increased at 3 dpa and decreased to its original value. These results suggested that the Nakagami and spectral Doppler imaging would be useful techniques in monitoring the regeneration of heart or tissues. MDPI 2019-09-22 /pmc/articles/PMC6806172/ /pubmed/31546705 http://dx.doi.org/10.3390/s19194094 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yeo, Sunmi Yoon, Changhan Lien, Ching-Ling Song, Tai-Kyong Shung, K. Kirk Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging |
title | Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging |
title_full | Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging |
title_fullStr | Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging |
title_full_unstemmed | Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging |
title_short | Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging |
title_sort | monitoring of adult zebrafish heart regeneration using high-frequency ultrasound spectral doppler and nakagami imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6806172/ https://www.ncbi.nlm.nih.gov/pubmed/31546705 http://dx.doi.org/10.3390/s19194094 |
work_keys_str_mv | AT yeosunmi monitoringofadultzebrafishheartregenerationusinghighfrequencyultrasoundspectraldopplerandnakagamiimaging AT yoonchanghan monitoringofadultzebrafishheartregenerationusinghighfrequencyultrasoundspectraldopplerandnakagamiimaging AT lienchingling monitoringofadultzebrafishheartregenerationusinghighfrequencyultrasoundspectraldopplerandnakagamiimaging AT songtaikyong monitoringofadultzebrafishheartregenerationusinghighfrequencyultrasoundspectraldopplerandnakagamiimaging AT shungkkirk monitoringofadultzebrafishheartregenerationusinghighfrequencyultrasoundspectraldopplerandnakagamiimaging |