Cargando…

Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging

This paper reports the feasibility of Nakagami imaging in monitoring the regeneration process of zebrafish hearts in a noninvasive manner. In addition, spectral Doppler waveforms that are typically used to access the diastolic function were measured to validate the performance of Nakagami imaging. A...

Descripción completa

Detalles Bibliográficos
Autores principales: Yeo, Sunmi, Yoon, Changhan, Lien, Ching-Ling, Song, Tai-Kyong, Shung, K. Kirk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6806172/
https://www.ncbi.nlm.nih.gov/pubmed/31546705
http://dx.doi.org/10.3390/s19194094
_version_ 1783461567240601600
author Yeo, Sunmi
Yoon, Changhan
Lien, Ching-Ling
Song, Tai-Kyong
Shung, K. Kirk
author_facet Yeo, Sunmi
Yoon, Changhan
Lien, Ching-Ling
Song, Tai-Kyong
Shung, K. Kirk
author_sort Yeo, Sunmi
collection PubMed
description This paper reports the feasibility of Nakagami imaging in monitoring the regeneration process of zebrafish hearts in a noninvasive manner. In addition, spectral Doppler waveforms that are typically used to access the diastolic function were measured to validate the performance of Nakagami imaging. A 30-MHz high-frequency ultrasound array transducer was used to acquire backscattered echo signal for spectral Doppler and Nakagami imaging. The performances of both methods were validated with flow and tissue-mimicking phantom experiments. For in vivo experiments, both spectral Doppler and Nakagami imaging were simultaneously obtained from adult zebrafish with amputated hearts. Longitudinal measurements were performed for five zebrafish. From the experiments, the E/A ratio measured using spectral Doppler imaging increased at 3 days post-amputation (3 dpa) and then decreased to the value before amputation, which were consistent with previous studies. Similar results were obtained from the Nakagami imaging where the Nakagami parameter value increased at 3 dpa and decreased to its original value. These results suggested that the Nakagami and spectral Doppler imaging would be useful techniques in monitoring the regeneration of heart or tissues.
format Online
Article
Text
id pubmed-6806172
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-68061722019-11-07 Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging Yeo, Sunmi Yoon, Changhan Lien, Ching-Ling Song, Tai-Kyong Shung, K. Kirk Sensors (Basel) Article This paper reports the feasibility of Nakagami imaging in monitoring the regeneration process of zebrafish hearts in a noninvasive manner. In addition, spectral Doppler waveforms that are typically used to access the diastolic function were measured to validate the performance of Nakagami imaging. A 30-MHz high-frequency ultrasound array transducer was used to acquire backscattered echo signal for spectral Doppler and Nakagami imaging. The performances of both methods were validated with flow and tissue-mimicking phantom experiments. For in vivo experiments, both spectral Doppler and Nakagami imaging were simultaneously obtained from adult zebrafish with amputated hearts. Longitudinal measurements were performed for five zebrafish. From the experiments, the E/A ratio measured using spectral Doppler imaging increased at 3 days post-amputation (3 dpa) and then decreased to the value before amputation, which were consistent with previous studies. Similar results were obtained from the Nakagami imaging where the Nakagami parameter value increased at 3 dpa and decreased to its original value. These results suggested that the Nakagami and spectral Doppler imaging would be useful techniques in monitoring the regeneration of heart or tissues. MDPI 2019-09-22 /pmc/articles/PMC6806172/ /pubmed/31546705 http://dx.doi.org/10.3390/s19194094 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yeo, Sunmi
Yoon, Changhan
Lien, Ching-Ling
Song, Tai-Kyong
Shung, K. Kirk
Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging
title Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging
title_full Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging
title_fullStr Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging
title_full_unstemmed Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging
title_short Monitoring of Adult Zebrafish Heart Regeneration Using High-Frequency Ultrasound Spectral Doppler and Nakagami Imaging
title_sort monitoring of adult zebrafish heart regeneration using high-frequency ultrasound spectral doppler and nakagami imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6806172/
https://www.ncbi.nlm.nih.gov/pubmed/31546705
http://dx.doi.org/10.3390/s19194094
work_keys_str_mv AT yeosunmi monitoringofadultzebrafishheartregenerationusinghighfrequencyultrasoundspectraldopplerandnakagamiimaging
AT yoonchanghan monitoringofadultzebrafishheartregenerationusinghighfrequencyultrasoundspectraldopplerandnakagamiimaging
AT lienchingling monitoringofadultzebrafishheartregenerationusinghighfrequencyultrasoundspectraldopplerandnakagamiimaging
AT songtaikyong monitoringofadultzebrafishheartregenerationusinghighfrequencyultrasoundspectraldopplerandnakagamiimaging
AT shungkkirk monitoringofadultzebrafishheartregenerationusinghighfrequencyultrasoundspectraldopplerandnakagamiimaging