Methods and applications of CRISPR/Cas system for genome editing in stem cells

Genome editing technology holds great promise for genome manipulation and gene therapy. While widespread utilization, genome editing has been used to unravel the roles of specific genes in differentiation and pluripotency of stem cells, and reinforce the stem cell-based applications. In this review,...

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Detalles Bibliográficos
Autores principales: Yang, Guang, Huang, Xingxu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Chinese Society for Cell Biology 2019
Materias:
Review Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6806369/
https://www.ncbi.nlm.nih.gov/pubmed/31666940
http://dx.doi.org/10.1016/j.cr.2019.08.001
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author Yang, Guang
Huang, Xingxu
author_facet Yang, Guang
Huang, Xingxu
author_sort Yang, Guang
collection PubMed
description Genome editing technology holds great promise for genome manipulation and gene therapy. While widespread utilization, genome editing has been used to unravel the roles of specific genes in differentiation and pluripotency of stem cells, and reinforce the stem cell-based applications. In this review, we summarize the advances of genome editing technology, as well as the derivative technologies from CRISPR/Cas system, which show tremendous potential in various fields. We also highlight the key findings in the studies of stem cells and regeneration by genome editing technology.
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spelling pubmed-68063692019-10-30 Methods and applications of CRISPR/Cas system for genome editing in stem cells Yang, Guang Huang, Xingxu Cell Regen Review Article Genome editing technology holds great promise for genome manipulation and gene therapy. While widespread utilization, genome editing has been used to unravel the roles of specific genes in differentiation and pluripotency of stem cells, and reinforce the stem cell-based applications. In this review, we summarize the advances of genome editing technology, as well as the derivative technologies from CRISPR/Cas system, which show tremendous potential in various fields. We also highlight the key findings in the studies of stem cells and regeneration by genome editing technology. Chinese Society for Cell Biology 2019-10-11 /pmc/articles/PMC6806369/ /pubmed/31666940 http://dx.doi.org/10.1016/j.cr.2019.08.001 Text en © 2019 Chinese Society for Cell Biology (CSCB). Production and hosting by Elsevier B.V. on behalf of KeAi. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Review Article
Yang, Guang
Huang, Xingxu
Methods and applications of CRISPR/Cas system for genome editing in stem cells
title Methods and applications of CRISPR/Cas system for genome editing in stem cells
title_full Methods and applications of CRISPR/Cas system for genome editing in stem cells
title_fullStr Methods and applications of CRISPR/Cas system for genome editing in stem cells
title_full_unstemmed Methods and applications of CRISPR/Cas system for genome editing in stem cells
title_short Methods and applications of CRISPR/Cas system for genome editing in stem cells
title_sort methods and applications of crispr/cas system for genome editing in stem cells
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6806369/
https://www.ncbi.nlm.nih.gov/pubmed/31666940
http://dx.doi.org/10.1016/j.cr.2019.08.001
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AT huangxingxu methodsandapplicationsofcrisprcassystemforgenomeeditinginstemcells

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