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Revealing the ultrastructure of the membrane pores of intact Serratia marcescens cells by atomic force microscopy
This study aimed to characterize the surface ultrastructure of intact Serratia marcescens cells under physiological conditions. Topographic information of membrane pores of the cells was obtained by atomic force microscope (AFM). Three types of membrane pores (CH-1-Pore A, CH-1-Pore B and CH-1-Pore...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6806401/ https://www.ncbi.nlm.nih.gov/pubmed/31692582 http://dx.doi.org/10.1016/j.heliyon.2019.e02636 |
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author | Lin, Yu-Chun Huang, Cheng Lai, Hsin-Chih |
author_facet | Lin, Yu-Chun Huang, Cheng Lai, Hsin-Chih |
author_sort | Lin, Yu-Chun |
collection | PubMed |
description | This study aimed to characterize the surface ultrastructure of intact Serratia marcescens cells under physiological conditions. Topographic information of membrane pores of the cells was obtained by atomic force microscope (AFM). Three types of membrane pores (CH-1-Pore A, CH-1-Pore B and CH-1-Pore C) were observed and the spatial arrangements of membrane-spanning subunits in membranes were defined. High-resolution images revealed that the doughnut-shaped structures of CH-1-Pore A and CH-1-Pore B were composed of six-to-eight and four transmembrane subunits. The inverted teepee-shaped structure of CH-1-Pore C was segmented into two transmembrane subunits straddling a single funnel-like pore. This study, to the best of authors' knowledge, represents the first direct characterization of the surface ultrastructure of the membrane pores of Serratia marcescens CH-1 cells at the nanometer scale and offers new prospects of mapping membrane pores on intact prokaryotic cells. |
format | Online Article Text |
id | pubmed-6806401 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-68064012019-11-05 Revealing the ultrastructure of the membrane pores of intact Serratia marcescens cells by atomic force microscopy Lin, Yu-Chun Huang, Cheng Lai, Hsin-Chih Heliyon Article This study aimed to characterize the surface ultrastructure of intact Serratia marcescens cells under physiological conditions. Topographic information of membrane pores of the cells was obtained by atomic force microscope (AFM). Three types of membrane pores (CH-1-Pore A, CH-1-Pore B and CH-1-Pore C) were observed and the spatial arrangements of membrane-spanning subunits in membranes were defined. High-resolution images revealed that the doughnut-shaped structures of CH-1-Pore A and CH-1-Pore B were composed of six-to-eight and four transmembrane subunits. The inverted teepee-shaped structure of CH-1-Pore C was segmented into two transmembrane subunits straddling a single funnel-like pore. This study, to the best of authors' knowledge, represents the first direct characterization of the surface ultrastructure of the membrane pores of Serratia marcescens CH-1 cells at the nanometer scale and offers new prospects of mapping membrane pores on intact prokaryotic cells. Elsevier 2019-10-15 /pmc/articles/PMC6806401/ /pubmed/31692582 http://dx.doi.org/10.1016/j.heliyon.2019.e02636 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Lin, Yu-Chun Huang, Cheng Lai, Hsin-Chih Revealing the ultrastructure of the membrane pores of intact Serratia marcescens cells by atomic force microscopy |
title | Revealing the ultrastructure of the membrane pores of intact Serratia marcescens cells by atomic force microscopy |
title_full | Revealing the ultrastructure of the membrane pores of intact Serratia marcescens cells by atomic force microscopy |
title_fullStr | Revealing the ultrastructure of the membrane pores of intact Serratia marcescens cells by atomic force microscopy |
title_full_unstemmed | Revealing the ultrastructure of the membrane pores of intact Serratia marcescens cells by atomic force microscopy |
title_short | Revealing the ultrastructure of the membrane pores of intact Serratia marcescens cells by atomic force microscopy |
title_sort | revealing the ultrastructure of the membrane pores of intact serratia marcescens cells by atomic force microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6806401/ https://www.ncbi.nlm.nih.gov/pubmed/31692582 http://dx.doi.org/10.1016/j.heliyon.2019.e02636 |
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