Cargando…

Revealing the ultrastructure of the membrane pores of intact Serratia marcescens cells by atomic force microscopy

This study aimed to characterize the surface ultrastructure of intact Serratia marcescens cells under physiological conditions. Topographic information of membrane pores of the cells was obtained by atomic force microscope (AFM). Three types of membrane pores (CH-1-Pore A, CH-1-Pore B and CH-1-Pore...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Yu-Chun, Huang, Cheng, Lai, Hsin-Chih
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6806401/
https://www.ncbi.nlm.nih.gov/pubmed/31692582
http://dx.doi.org/10.1016/j.heliyon.2019.e02636
_version_ 1783461620177960960
author Lin, Yu-Chun
Huang, Cheng
Lai, Hsin-Chih
author_facet Lin, Yu-Chun
Huang, Cheng
Lai, Hsin-Chih
author_sort Lin, Yu-Chun
collection PubMed
description This study aimed to characterize the surface ultrastructure of intact Serratia marcescens cells under physiological conditions. Topographic information of membrane pores of the cells was obtained by atomic force microscope (AFM). Three types of membrane pores (CH-1-Pore A, CH-1-Pore B and CH-1-Pore C) were observed and the spatial arrangements of membrane-spanning subunits in membranes were defined. High-resolution images revealed that the doughnut-shaped structures of CH-1-Pore A and CH-1-Pore B were composed of six-to-eight and four transmembrane subunits. The inverted teepee-shaped structure of CH-1-Pore C was segmented into two transmembrane subunits straddling a single funnel-like pore. This study, to the best of authors' knowledge, represents the first direct characterization of the surface ultrastructure of the membrane pores of Serratia marcescens CH-1 cells at the nanometer scale and offers new prospects of mapping membrane pores on intact prokaryotic cells.
format Online
Article
Text
id pubmed-6806401
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-68064012019-11-05 Revealing the ultrastructure of the membrane pores of intact Serratia marcescens cells by atomic force microscopy Lin, Yu-Chun Huang, Cheng Lai, Hsin-Chih Heliyon Article This study aimed to characterize the surface ultrastructure of intact Serratia marcescens cells under physiological conditions. Topographic information of membrane pores of the cells was obtained by atomic force microscope (AFM). Three types of membrane pores (CH-1-Pore A, CH-1-Pore B and CH-1-Pore C) were observed and the spatial arrangements of membrane-spanning subunits in membranes were defined. High-resolution images revealed that the doughnut-shaped structures of CH-1-Pore A and CH-1-Pore B were composed of six-to-eight and four transmembrane subunits. The inverted teepee-shaped structure of CH-1-Pore C was segmented into two transmembrane subunits straddling a single funnel-like pore. This study, to the best of authors' knowledge, represents the first direct characterization of the surface ultrastructure of the membrane pores of Serratia marcescens CH-1 cells at the nanometer scale and offers new prospects of mapping membrane pores on intact prokaryotic cells. Elsevier 2019-10-15 /pmc/articles/PMC6806401/ /pubmed/31692582 http://dx.doi.org/10.1016/j.heliyon.2019.e02636 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Lin, Yu-Chun
Huang, Cheng
Lai, Hsin-Chih
Revealing the ultrastructure of the membrane pores of intact Serratia marcescens cells by atomic force microscopy
title Revealing the ultrastructure of the membrane pores of intact Serratia marcescens cells by atomic force microscopy
title_full Revealing the ultrastructure of the membrane pores of intact Serratia marcescens cells by atomic force microscopy
title_fullStr Revealing the ultrastructure of the membrane pores of intact Serratia marcescens cells by atomic force microscopy
title_full_unstemmed Revealing the ultrastructure of the membrane pores of intact Serratia marcescens cells by atomic force microscopy
title_short Revealing the ultrastructure of the membrane pores of intact Serratia marcescens cells by atomic force microscopy
title_sort revealing the ultrastructure of the membrane pores of intact serratia marcescens cells by atomic force microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6806401/
https://www.ncbi.nlm.nih.gov/pubmed/31692582
http://dx.doi.org/10.1016/j.heliyon.2019.e02636
work_keys_str_mv AT linyuchun revealingtheultrastructureofthemembraneporesofintactserratiamarcescenscellsbyatomicforcemicroscopy
AT huangcheng revealingtheultrastructureofthemembraneporesofintactserratiamarcescenscellsbyatomicforcemicroscopy
AT laihsinchih revealingtheultrastructureofthemembraneporesofintactserratiamarcescenscellsbyatomicforcemicroscopy