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Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein
Generation of stable cell lines is a widely used technique for continuous recombinant protein production. Advantages of the constitutive stable over the transient protein expression are uniformity of the expression across cell populations as well as high quantity and consistency of the protein yield...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6806449/ https://www.ncbi.nlm.nih.gov/pubmed/31656839 http://dx.doi.org/10.1016/j.dib.2019.104603 |
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author | Hansoongnern, Payuda Kaewborisuth, Challika Lekcharoensuk, Porntippa |
author_facet | Hansoongnern, Payuda Kaewborisuth, Challika Lekcharoensuk, Porntippa |
author_sort | Hansoongnern, Payuda |
collection | PubMed |
description | Generation of stable cell lines is a widely used technique for continuous recombinant protein production. Advantages of the constitutive stable over the transient protein expression are uniformity of the expression across cell populations as well as high quantity and consistency of the protein yields. This data describe step-by-step procedure for the production of glycoprotein without a transmembrane domain (GΔTM) of bovine ephemeral fever virus (BEFV) by mammalian stable cells. LentiX-293T cells were transfected with four plasmid constructs to generate a recombinant lentivirus. Subsequently, 293T cells were transduced by the recombinant virus and the polyclonal stable cell pools were then selected by puromycin. Next, limiting dilution was performed from each cell pool to isolate the monoclonal stable cells expressing GΔTM protein. Western blot analysis showed that all monoclonal cell clones could stably express GΔTM protein. The data confirms that the stable 293T cell line expressing the secretory GΔTM protein is an attractive platform for antigen production. |
format | Online Article Text |
id | pubmed-6806449 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-68064492019-10-25 Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein Hansoongnern, Payuda Kaewborisuth, Challika Lekcharoensuk, Porntippa Data Brief Immunology and Microbiology Generation of stable cell lines is a widely used technique for continuous recombinant protein production. Advantages of the constitutive stable over the transient protein expression are uniformity of the expression across cell populations as well as high quantity and consistency of the protein yields. This data describe step-by-step procedure for the production of glycoprotein without a transmembrane domain (GΔTM) of bovine ephemeral fever virus (BEFV) by mammalian stable cells. LentiX-293T cells were transfected with four plasmid constructs to generate a recombinant lentivirus. Subsequently, 293T cells were transduced by the recombinant virus and the polyclonal stable cell pools were then selected by puromycin. Next, limiting dilution was performed from each cell pool to isolate the monoclonal stable cells expressing GΔTM protein. Western blot analysis showed that all monoclonal cell clones could stably express GΔTM protein. The data confirms that the stable 293T cell line expressing the secretory GΔTM protein is an attractive platform for antigen production. Elsevier 2019-10-03 /pmc/articles/PMC6806449/ /pubmed/31656839 http://dx.doi.org/10.1016/j.dib.2019.104603 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Immunology and Microbiology Hansoongnern, Payuda Kaewborisuth, Challika Lekcharoensuk, Porntippa Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein |
title | Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein |
title_full | Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein |
title_fullStr | Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein |
title_full_unstemmed | Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein |
title_short | Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein |
title_sort | data on production of mammalian stable cells expressing secretory befv transmembrane deleted g protein |
topic | Immunology and Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6806449/ https://www.ncbi.nlm.nih.gov/pubmed/31656839 http://dx.doi.org/10.1016/j.dib.2019.104603 |
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