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Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein

Generation of stable cell lines is a widely used technique for continuous recombinant protein production. Advantages of the constitutive stable over the transient protein expression are uniformity of the expression across cell populations as well as high quantity and consistency of the protein yield...

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Autores principales: Hansoongnern, Payuda, Kaewborisuth, Challika, Lekcharoensuk, Porntippa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6806449/
https://www.ncbi.nlm.nih.gov/pubmed/31656839
http://dx.doi.org/10.1016/j.dib.2019.104603
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author Hansoongnern, Payuda
Kaewborisuth, Challika
Lekcharoensuk, Porntippa
author_facet Hansoongnern, Payuda
Kaewborisuth, Challika
Lekcharoensuk, Porntippa
author_sort Hansoongnern, Payuda
collection PubMed
description Generation of stable cell lines is a widely used technique for continuous recombinant protein production. Advantages of the constitutive stable over the transient protein expression are uniformity of the expression across cell populations as well as high quantity and consistency of the protein yields. This data describe step-by-step procedure for the production of glycoprotein without a transmembrane domain (GΔTM) of bovine ephemeral fever virus (BEFV) by mammalian stable cells. LentiX-293T cells were transfected with four plasmid constructs to generate a recombinant lentivirus. Subsequently, 293T cells were transduced by the recombinant virus and the polyclonal stable cell pools were then selected by puromycin. Next, limiting dilution was performed from each cell pool to isolate the monoclonal stable cells expressing GΔTM protein. Western blot analysis showed that all monoclonal cell clones could stably express GΔTM protein. The data confirms that the stable 293T cell line expressing the secretory GΔTM protein is an attractive platform for antigen production.
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spelling pubmed-68064492019-10-25 Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein Hansoongnern, Payuda Kaewborisuth, Challika Lekcharoensuk, Porntippa Data Brief Immunology and Microbiology Generation of stable cell lines is a widely used technique for continuous recombinant protein production. Advantages of the constitutive stable over the transient protein expression are uniformity of the expression across cell populations as well as high quantity and consistency of the protein yields. This data describe step-by-step procedure for the production of glycoprotein without a transmembrane domain (GΔTM) of bovine ephemeral fever virus (BEFV) by mammalian stable cells. LentiX-293T cells were transfected with four plasmid constructs to generate a recombinant lentivirus. Subsequently, 293T cells were transduced by the recombinant virus and the polyclonal stable cell pools were then selected by puromycin. Next, limiting dilution was performed from each cell pool to isolate the monoclonal stable cells expressing GΔTM protein. Western blot analysis showed that all monoclonal cell clones could stably express GΔTM protein. The data confirms that the stable 293T cell line expressing the secretory GΔTM protein is an attractive platform for antigen production. Elsevier 2019-10-03 /pmc/articles/PMC6806449/ /pubmed/31656839 http://dx.doi.org/10.1016/j.dib.2019.104603 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Immunology and Microbiology
Hansoongnern, Payuda
Kaewborisuth, Challika
Lekcharoensuk, Porntippa
Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein
title Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein
title_full Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein
title_fullStr Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein
title_full_unstemmed Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein
title_short Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein
title_sort data on production of mammalian stable cells expressing secretory befv transmembrane deleted g protein
topic Immunology and Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6806449/
https://www.ncbi.nlm.nih.gov/pubmed/31656839
http://dx.doi.org/10.1016/j.dib.2019.104603
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