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2849. Gut Microbiota Differences at the Time of Medical Intensive Care Unit (MICU) Admission Are Associated with Acquisition of Multi-drug-Resistant Organisms (MDROs) Among Patients Not Already Colonized with an MDRO
BACKGROUND: Among hospitalized patients, underlying variation in gut microbiota may confer differential risk for gut MDRO acquisition. METHODS: Rectal swab samples were collected from patients ≤2 days of MICU admission and then daily in the 27-bed MICU of an acute care hospital in Chicago, IL over 1...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6808848/ http://dx.doi.org/10.1093/ofid/ofz359.154 |
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author | Bassis, Christine Seekatz, Anna Dangana, Thelma E Shimasaki, Teppei Yelin, Rachel D Schoeny, Michael Rhee, Yoona Ariston, Michelle Lolans, Karen Cornejo Cisneros, Enrique Aboushaala, Khaled Thabit, Lina Murray, John Sheng, Jianrong Ollison, Stefanie Bell, Pamela B Fogg, Louis Weinstein, Robert A Lin, Michael Y Young, Vincent B Hayden, Mary K |
author_facet | Bassis, Christine Seekatz, Anna Dangana, Thelma E Shimasaki, Teppei Yelin, Rachel D Schoeny, Michael Rhee, Yoona Ariston, Michelle Lolans, Karen Cornejo Cisneros, Enrique Aboushaala, Khaled Thabit, Lina Murray, John Sheng, Jianrong Ollison, Stefanie Bell, Pamela B Fogg, Louis Weinstein, Robert A Lin, Michael Y Young, Vincent B Hayden, Mary K |
author_sort | Bassis, Christine |
collection | PubMed |
description | BACKGROUND: Among hospitalized patients, underlying variation in gut microbiota may confer differential risk for gut MDRO acquisition. METHODS: Rectal swab samples were collected from patients ≤2 days of MICU admission and then daily in the 27-bed MICU of an acute care hospital in Chicago, IL over 1 year. Patients were screened for MDRO colonization by selective culture (see Figure 1 for MDRO types); those with ≥2 swabs and MICU stays ≥3 days were studied. Bacterial 16S rRNA gene amplicon sequences were used for microbiota analysis. Medical records were reviewed. RESULTS: In preliminary analysis, 2,480 samples were collected from 627 patients who acquired 170 MDROs (Figure 1). Debilitation, co-morbidities, and certain medical devices were associated with MDRO acquisition, though admission MDRO status was not (table). While no interactions were detected between admission MDRO status and clinical predictors of MDRO acquisition, there were significant differences in gut microbiota composition at the time of MICU admission between patients colonized with an MDRO on admission and those not colonized (P < 0.001, using analysis of molecular variance (AMOVA) on distances). Therefore, we stratified our analysis by admission MDRO colonization status. For patients MDRO-colonized at admission, there were no significant differences in microbiota of patients who later did or did not acquire a new MDRO (AMOVA P-value = 0.32). For patients not MDRO-colonized on admission, there was a significant difference in microbiota of patients who later acquired an MDRO and those who did not (AMOVA P-value: 0.026). Differentially abundant operational taxonomic units (OTUs, based on 3% sequence difference) included OTUs classified as Anaerococcus and as other Clostridiales (higher in patients who remained uncolonized) and as Enterococcus (higher in patients who acquired an MDRO) (Figure 2). Diversity was also higher in patients who remained uncolonized (Wilcoxon test P-value: 0.035) (Figure 3). CONCLUSION: Among patients not already colonized with an MDRO on admission, we identified gut microbiota differences associated with MDRO acquisition that could help explain patient-level variation in MDRO colonization resistance. [Image: see text] [Image: see text] [Image: see text] [Image: see text] DISCLOSURES: All Authors: No reported Disclosures. |
format | Online Article Text |
id | pubmed-6808848 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-68088482019-10-28 2849. Gut Microbiota Differences at the Time of Medical Intensive Care Unit (MICU) Admission Are Associated with Acquisition of Multi-drug-Resistant Organisms (MDROs) Among Patients Not Already Colonized with an MDRO Bassis, Christine Seekatz, Anna Dangana, Thelma E Shimasaki, Teppei Yelin, Rachel D Schoeny, Michael Rhee, Yoona Ariston, Michelle Lolans, Karen Cornejo Cisneros, Enrique Aboushaala, Khaled Thabit, Lina Murray, John Sheng, Jianrong Ollison, Stefanie Bell, Pamela B Fogg, Louis Weinstein, Robert A Lin, Michael Y Young, Vincent B Hayden, Mary K Open Forum Infect Dis Abstracts BACKGROUND: Among hospitalized patients, underlying variation in gut microbiota may confer differential risk for gut MDRO acquisition. METHODS: Rectal swab samples were collected from patients ≤2 days of MICU admission and then daily in the 27-bed MICU of an acute care hospital in Chicago, IL over 1 year. Patients were screened for MDRO colonization by selective culture (see Figure 1 for MDRO types); those with ≥2 swabs and MICU stays ≥3 days were studied. Bacterial 16S rRNA gene amplicon sequences were used for microbiota analysis. Medical records were reviewed. RESULTS: In preliminary analysis, 2,480 samples were collected from 627 patients who acquired 170 MDROs (Figure 1). Debilitation, co-morbidities, and certain medical devices were associated with MDRO acquisition, though admission MDRO status was not (table). While no interactions were detected between admission MDRO status and clinical predictors of MDRO acquisition, there were significant differences in gut microbiota composition at the time of MICU admission between patients colonized with an MDRO on admission and those not colonized (P < 0.001, using analysis of molecular variance (AMOVA) on distances). Therefore, we stratified our analysis by admission MDRO colonization status. For patients MDRO-colonized at admission, there were no significant differences in microbiota of patients who later did or did not acquire a new MDRO (AMOVA P-value = 0.32). For patients not MDRO-colonized on admission, there was a significant difference in microbiota of patients who later acquired an MDRO and those who did not (AMOVA P-value: 0.026). Differentially abundant operational taxonomic units (OTUs, based on 3% sequence difference) included OTUs classified as Anaerococcus and as other Clostridiales (higher in patients who remained uncolonized) and as Enterococcus (higher in patients who acquired an MDRO) (Figure 2). Diversity was also higher in patients who remained uncolonized (Wilcoxon test P-value: 0.035) (Figure 3). CONCLUSION: Among patients not already colonized with an MDRO on admission, we identified gut microbiota differences associated with MDRO acquisition that could help explain patient-level variation in MDRO colonization resistance. [Image: see text] [Image: see text] [Image: see text] [Image: see text] DISCLOSURES: All Authors: No reported Disclosures. Oxford University Press 2019-10-23 /pmc/articles/PMC6808848/ http://dx.doi.org/10.1093/ofid/ofz359.154 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Abstracts Bassis, Christine Seekatz, Anna Dangana, Thelma E Shimasaki, Teppei Yelin, Rachel D Schoeny, Michael Rhee, Yoona Ariston, Michelle Lolans, Karen Cornejo Cisneros, Enrique Aboushaala, Khaled Thabit, Lina Murray, John Sheng, Jianrong Ollison, Stefanie Bell, Pamela B Fogg, Louis Weinstein, Robert A Lin, Michael Y Young, Vincent B Hayden, Mary K 2849. Gut Microbiota Differences at the Time of Medical Intensive Care Unit (MICU) Admission Are Associated with Acquisition of Multi-drug-Resistant Organisms (MDROs) Among Patients Not Already Colonized with an MDRO |
title | 2849. Gut Microbiota Differences at the Time of Medical Intensive Care Unit (MICU) Admission Are Associated with Acquisition of Multi-drug-Resistant Organisms (MDROs) Among Patients Not Already Colonized with an MDRO |
title_full | 2849. Gut Microbiota Differences at the Time of Medical Intensive Care Unit (MICU) Admission Are Associated with Acquisition of Multi-drug-Resistant Organisms (MDROs) Among Patients Not Already Colonized with an MDRO |
title_fullStr | 2849. Gut Microbiota Differences at the Time of Medical Intensive Care Unit (MICU) Admission Are Associated with Acquisition of Multi-drug-Resistant Organisms (MDROs) Among Patients Not Already Colonized with an MDRO |
title_full_unstemmed | 2849. Gut Microbiota Differences at the Time of Medical Intensive Care Unit (MICU) Admission Are Associated with Acquisition of Multi-drug-Resistant Organisms (MDROs) Among Patients Not Already Colonized with an MDRO |
title_short | 2849. Gut Microbiota Differences at the Time of Medical Intensive Care Unit (MICU) Admission Are Associated with Acquisition of Multi-drug-Resistant Organisms (MDROs) Among Patients Not Already Colonized with an MDRO |
title_sort | 2849. gut microbiota differences at the time of medical intensive care unit (micu) admission are associated with acquisition of multi-drug-resistant organisms (mdros) among patients not already colonized with an mdro |
topic | Abstracts |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6808848/ http://dx.doi.org/10.1093/ofid/ofz359.154 |
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