1786. An Automated Method to Assess Oligonucleotide Primer and Probe Complementarity to Genomic Targets in Infectious Disease qPCR Assays

BACKGROUND: Success of real-time TaqMan PCR (qPCR) in detecting pathogen targets and quantifying pathogen load is dependent upon frequent assay monitoring. This is due to i) the high degree of complementarity needed between primers / probes and genomic targets for assay accuracy and ii) natural path...

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Autores principales: Sinha, Rohita, Wissel, Mark, Bartlett, Katelyn, Grantham, James, Kleiboeker, Steve
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
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Abstracts
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6809443/
http://dx.doi.org/10.1093/ofid/ofz360.1649

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