2584. Effects of Fecal Microbiota Transplantation for Decolonizing Multidrug-Resistant Organism

BACKGROUND: Increasing prevalence of multidrug-resistant microorganisms (MDRO) results in poor clinical outcomes, longer hospitalizations and higher healthcare costs. It is likely that MDRO colonization can lead infections to vulnerable patients. Currently, however, MDRO decolonization strategies are lacking. The purpose of this study was to prove the efficacy of FMT on decolonization of carbapenemase-producing Enterobacteriaceae (CPE) and vancomycin-resistant enterococci (VRE) carriers. METHODS: This study was a prospective, open-label, uncontrolled, single-center pilot study of FMT for digestive tract colonized CPE, VRE, or CPE/VRE patients between March 2018 and February 2019. Fecal solution obtained from healthy unrelated donors was infused to recipient’s gut. Fecal samples of recipients were collected before and after FMT until 1year.We compared characteristics of subjects succeed in decolonization during study period (responders) with subjects who failed to decolonize MDRO by FMT (non-responders). Furthermore, microbiome analyses were performed to investigate the influence of microbial characteristics of recipients on the outcome of FMT. RESULTS: Decolonization was achieved in 12/23 (52.2%) during study period. Hemoglobin (11.0 vs. 10.0, P = 0.018), low-density lipoprotein cholesterol (102.0 vs. 89.0, P = 0.049), and albumin (3.4 vs. 3.2, P = 0.006) levels were higher in responders. Antibiotic treatment(ATB) within 1 week after FMT were less common in responders (41.7% vs. 90.9%, P = 0.027). Patients with no ATB approached frequent decolonization at 1(75.0% vs. 26.7%; P = 0.037) and 3 months (87.5% vs. 33.3%; P = 0.027). The rates of decolonization were significantly different between CPE, VRE and CPE/VRE colonizer (75.0% vs. 38.5% vs. 66.7%; P = 0.018). Gut microbiome of responders showed a higher richness and diversity than non-responders before(294 vs. 274 by Ace; 2.6 vs. 1.8 by Shannon) and after (345 vs. 260 by Ace; 2.9 vs. 2.1 by Shannon) FMT. The microbiota composition analysis revealed increasing abundance of Bacteroidetes and decreasing abundance of Proteobacteria at 1 month after FMT in responders. However, those changes of microbial composition did not occur in non-responders. CONCLUSION: FMT is an effective way to decolonize CPE and VRE by restoration of the gut microbiome. [Image: see text] [Image: see text] [Image: see text] [Image: see text] DISCLOSURES: All authors: No reported disclosures.