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2649. Measles-Containing Vaccination Resulted in a Balanced Cytokine Profile Without Evidence of Immunosuppression in Healthy 12-Month-Old Children

BACKGROUND: Measles virus infection results in immune activation, viral clearance and lifelong immunity. In addition, there is an immunosuppressive state defined by type 2 skewing of CD4(+) T‐cell cytokine production and induction of regulatory T cells with reduced dendritic cell (DC) activation in...

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Autores principales: Gans, Hayley, Wang, Weiqi, Rosenberg-Hasson, Yael, Zerbo, Ousseny, Modaressi, Sharareh, Goddard, Kristin, Lewis, Ned, Bok, Karin, Klein, Nicola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6810256/
http://dx.doi.org/10.1093/ofid/ofz360.2327
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author Gans, Hayley
Wang, Weiqi
Rosenberg-Hasson, Yael
Zerbo, Ousseny
Modaressi, Sharareh
Goddard, Kristin
Lewis, Ned
Bok, Karin
Klein, Nicola
author_facet Gans, Hayley
Wang, Weiqi
Rosenberg-Hasson, Yael
Zerbo, Ousseny
Modaressi, Sharareh
Goddard, Kristin
Lewis, Ned
Bok, Karin
Klein, Nicola
author_sort Gans, Hayley
collection PubMed
description BACKGROUND: Measles virus infection results in immune activation, viral clearance and lifelong immunity. In addition, there is an immunosuppressive state defined by type 2 skewing of CD4(+) T‐cell cytokine production and induction of regulatory T cells with reduced dendritic cell (DC) activation in the recovery phase. Studies following measles immunization show conflicting immune profiles. To more robustly interrogate and define specific functional cytokine profiles, this study evaluated cytokine profiles in 12-month old infants before and after primary MMR vaccination. METHODS: Cytokine profiles using luminex assay (62-plex; eBioscience) were measured in 65 infants before and 42 days after MMR vaccination administered at 12 months of age as part of a randomized clinical trial. Mean cytokine percentages of children with increased or decreased concentrations of each cytokine in the post sample compared with the levels in the pre sample were evaluated using Student’s t-test. Cytokines were arranged into dominant CD4+ T-cell type, Th1, Th2, and T regulatory (T(reg)) and those produced by DC. RESULTS: No dominant cytokine pattern emerged following measles immunization, with a balanced profile. The mean percentage of children with increased and decreased concentrations (pg/mL) of signature CD 4+ T-cell Th1 (tumor necrosis factor alpha [TNFa], interferon gamma [IFNg]), Th2 (Interleukin [IL] IL5, IL4, IL13), T(reg) (IL10, transforming growth factor-β TCFb) and DC (IL12P40 and IL12P70) cytokines were equivalent when measured at 42 days after MMR vaccine compared with levels before vaccine (Table 1) (P ≥ 0.05 for all comparisons). CONCLUSION: In contrast to data demonstrating an immune suppression profile following measles disease, measles-containing vaccine did not suppress Th1 CD4+ T-cell and DC cytokines or promote Th2 and T(reg) CD4+ T-cell cytokines measured 42 days after vaccination. The cytokine profile represents one of balance and homeostasis. This study supports the data that show measles vaccine does not cause immunosuppression in healthy infants. [Image: see text] DISCLOSURES: All authors: No reported disclosures.
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spelling pubmed-68102562019-10-28 2649. Measles-Containing Vaccination Resulted in a Balanced Cytokine Profile Without Evidence of Immunosuppression in Healthy 12-Month-Old Children Gans, Hayley Wang, Weiqi Rosenberg-Hasson, Yael Zerbo, Ousseny Modaressi, Sharareh Goddard, Kristin Lewis, Ned Bok, Karin Klein, Nicola Open Forum Infect Dis Abstracts BACKGROUND: Measles virus infection results in immune activation, viral clearance and lifelong immunity. In addition, there is an immunosuppressive state defined by type 2 skewing of CD4(+) T‐cell cytokine production and induction of regulatory T cells with reduced dendritic cell (DC) activation in the recovery phase. Studies following measles immunization show conflicting immune profiles. To more robustly interrogate and define specific functional cytokine profiles, this study evaluated cytokine profiles in 12-month old infants before and after primary MMR vaccination. METHODS: Cytokine profiles using luminex assay (62-plex; eBioscience) were measured in 65 infants before and 42 days after MMR vaccination administered at 12 months of age as part of a randomized clinical trial. Mean cytokine percentages of children with increased or decreased concentrations of each cytokine in the post sample compared with the levels in the pre sample were evaluated using Student’s t-test. Cytokines were arranged into dominant CD4+ T-cell type, Th1, Th2, and T regulatory (T(reg)) and those produced by DC. RESULTS: No dominant cytokine pattern emerged following measles immunization, with a balanced profile. The mean percentage of children with increased and decreased concentrations (pg/mL) of signature CD 4+ T-cell Th1 (tumor necrosis factor alpha [TNFa], interferon gamma [IFNg]), Th2 (Interleukin [IL] IL5, IL4, IL13), T(reg) (IL10, transforming growth factor-β TCFb) and DC (IL12P40 and IL12P70) cytokines were equivalent when measured at 42 days after MMR vaccine compared with levels before vaccine (Table 1) (P ≥ 0.05 for all comparisons). CONCLUSION: In contrast to data demonstrating an immune suppression profile following measles disease, measles-containing vaccine did not suppress Th1 CD4+ T-cell and DC cytokines or promote Th2 and T(reg) CD4+ T-cell cytokines measured 42 days after vaccination. The cytokine profile represents one of balance and homeostasis. This study supports the data that show measles vaccine does not cause immunosuppression in healthy infants. [Image: see text] DISCLOSURES: All authors: No reported disclosures. Oxford University Press 2019-10-23 /pmc/articles/PMC6810256/ http://dx.doi.org/10.1093/ofid/ofz360.2327 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Abstracts
Gans, Hayley
Wang, Weiqi
Rosenberg-Hasson, Yael
Zerbo, Ousseny
Modaressi, Sharareh
Goddard, Kristin
Lewis, Ned
Bok, Karin
Klein, Nicola
2649. Measles-Containing Vaccination Resulted in a Balanced Cytokine Profile Without Evidence of Immunosuppression in Healthy 12-Month-Old Children
title 2649. Measles-Containing Vaccination Resulted in a Balanced Cytokine Profile Without Evidence of Immunosuppression in Healthy 12-Month-Old Children
title_full 2649. Measles-Containing Vaccination Resulted in a Balanced Cytokine Profile Without Evidence of Immunosuppression in Healthy 12-Month-Old Children
title_fullStr 2649. Measles-Containing Vaccination Resulted in a Balanced Cytokine Profile Without Evidence of Immunosuppression in Healthy 12-Month-Old Children
title_full_unstemmed 2649. Measles-Containing Vaccination Resulted in a Balanced Cytokine Profile Without Evidence of Immunosuppression in Healthy 12-Month-Old Children
title_short 2649. Measles-Containing Vaccination Resulted in a Balanced Cytokine Profile Without Evidence of Immunosuppression in Healthy 12-Month-Old Children
title_sort 2649. measles-containing vaccination resulted in a balanced cytokine profile without evidence of immunosuppression in healthy 12-month-old children
topic Abstracts
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6810256/
http://dx.doi.org/10.1093/ofid/ofz360.2327
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