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556. Phylogenomic Epidemiology of Methicillin-Resistant Staphylococcus aureus (MRSA) Chilean-Cordobes Clone in Latin America

BACKGROUND: The MRSA Chilean-Cordobes (ChC) clone belongs to the clonal complex 5 (CC5) and typically carries SCCmec I. The ChC clone predominated widely throughout several countries of Latin America (LA), but during the mid-2000s a CA-MRSA CC8 LA variant (USA300-LV) quickly replaced the ChC in Colo...

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Autores principales: Martínez, Jose R W, Diaz, Lorena, Rojas, Marcelo, Rios, Rafael, Hanson, Blake, Rivas, Lina M, Spencer, Maria, Moustafa, Ahmed M, Araos Bralic, Rafael, Peters, Anne, Reyes, Jinnethe, Carvajal, Lina P, Luna, Carlos, Salles, Mauro, Alvarez, Carlos, Labarca, Jaime, Seas, Carlos, Guzmán, Manuel, Planet, Paul J, Arias, Cesar A, Munita, Jose
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6811053/
http://dx.doi.org/10.1093/ofid/ofz360.625
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author Martínez, Jose R W
Diaz, Lorena
Rojas, Marcelo
Rios, Rafael
Hanson, Blake
Rivas, Lina M
Spencer, Maria
Moustafa, Ahmed M
Araos Bralic, Rafael
Peters, Anne
Reyes, Jinnethe
Carvajal, Lina P
Luna, Carlos
Salles, Mauro
Alvarez, Carlos
Labarca, Jaime
Seas, Carlos
Seas, Carlos
Guzmán, Manuel
Planet, Paul J
Planet, Paul J
Arias, Cesar A
Munita, Jose
author_facet Martínez, Jose R W
Diaz, Lorena
Rojas, Marcelo
Rios, Rafael
Hanson, Blake
Rivas, Lina M
Spencer, Maria
Moustafa, Ahmed M
Araos Bralic, Rafael
Peters, Anne
Reyes, Jinnethe
Carvajal, Lina P
Luna, Carlos
Salles, Mauro
Alvarez, Carlos
Labarca, Jaime
Seas, Carlos
Seas, Carlos
Guzmán, Manuel
Planet, Paul J
Planet, Paul J
Arias, Cesar A
Munita, Jose
author_sort Martínez, Jose R W
collection PubMed
description BACKGROUND: The MRSA Chilean-Cordobes (ChC) clone belongs to the clonal complex 5 (CC5) and typically carries SCCmec I. The ChC clone predominated widely throughout several countries of Latin America (LA), but during the mid-2000s a CA-MRSA CC8 LA variant (USA300-LV) quickly replaced the ChC in Colombia and Ecuador. Most notably, this replacement was not observed in Peru or Chile. Here, we aimed to understand the phylogenomic relatedness of the CC5 ChC clone obtained from different countries of LA. METHODS: We sequenced and analyzed the genomes of 115 MRSA isolates obtained between 2011–2014 from bloodstream infections in 6 LA countries (Argentina, Brazil, Colombia, Chile, Peru, and Venezuela). All isolates were confirmed as ChC clone by pulsed-field gel electrophoresis (PFGE). We used core genome-based phylogenomic reconstructions and molecular clock analysis to infer the relationships and time of divergence between clades. RESULTS: Whole-genome-based multilocus sequence typing determined that 110/115 isolates belonged to ST5 and carried SCCmec I. The phylogenomic reconstruction showed ChC isolates clustered into 4 major clades distinctly segregated by country of origin (Figure 1). Interestingly, isolates recovered from Chile divided into 2 different clades that segregate according to the city of origin (Santiago [SCL] or Concepción [CON]), suggesting these clades evolved independently. Molecular clock analyses suggested all clades share a common ancestor with the divergence of the Chilean clades occurring earlier (Figure 2). Of note, analysis of heavy metal genes suggested the divergence between Chilean isolates was characterized by the loss of a mercury resistance gene cluster, which is present in an 88% of CON isolates, but only in 28% of SCL (Figure 2). CONCLUSION: MRSA isolates belonging to the ChC clone from 6 LA countries clustered in 4 clades according to the geographical region of isolation. This segregation suggests divergent adaptations that may respond to different selective pressures. Heavy metal resistance could play a role in the ability of the MRSA ChC to disseminate in specific geographical locations. [Image: see text] [Image: see text] DISCLOSURES: All authors: No reported disclosures.
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spelling pubmed-68110532019-10-28 556. Phylogenomic Epidemiology of Methicillin-Resistant Staphylococcus aureus (MRSA) Chilean-Cordobes Clone in Latin America Martínez, Jose R W Diaz, Lorena Rojas, Marcelo Rios, Rafael Hanson, Blake Rivas, Lina M Spencer, Maria Moustafa, Ahmed M Araos Bralic, Rafael Peters, Anne Reyes, Jinnethe Carvajal, Lina P Luna, Carlos Salles, Mauro Alvarez, Carlos Labarca, Jaime Seas, Carlos Seas, Carlos Guzmán, Manuel Planet, Paul J Planet, Paul J Arias, Cesar A Munita, Jose Open Forum Infect Dis Abstracts BACKGROUND: The MRSA Chilean-Cordobes (ChC) clone belongs to the clonal complex 5 (CC5) and typically carries SCCmec I. The ChC clone predominated widely throughout several countries of Latin America (LA), but during the mid-2000s a CA-MRSA CC8 LA variant (USA300-LV) quickly replaced the ChC in Colombia and Ecuador. Most notably, this replacement was not observed in Peru or Chile. Here, we aimed to understand the phylogenomic relatedness of the CC5 ChC clone obtained from different countries of LA. METHODS: We sequenced and analyzed the genomes of 115 MRSA isolates obtained between 2011–2014 from bloodstream infections in 6 LA countries (Argentina, Brazil, Colombia, Chile, Peru, and Venezuela). All isolates were confirmed as ChC clone by pulsed-field gel electrophoresis (PFGE). We used core genome-based phylogenomic reconstructions and molecular clock analysis to infer the relationships and time of divergence between clades. RESULTS: Whole-genome-based multilocus sequence typing determined that 110/115 isolates belonged to ST5 and carried SCCmec I. The phylogenomic reconstruction showed ChC isolates clustered into 4 major clades distinctly segregated by country of origin (Figure 1). Interestingly, isolates recovered from Chile divided into 2 different clades that segregate according to the city of origin (Santiago [SCL] or Concepción [CON]), suggesting these clades evolved independently. Molecular clock analyses suggested all clades share a common ancestor with the divergence of the Chilean clades occurring earlier (Figure 2). Of note, analysis of heavy metal genes suggested the divergence between Chilean isolates was characterized by the loss of a mercury resistance gene cluster, which is present in an 88% of CON isolates, but only in 28% of SCL (Figure 2). CONCLUSION: MRSA isolates belonging to the ChC clone from 6 LA countries clustered in 4 clades according to the geographical region of isolation. This segregation suggests divergent adaptations that may respond to different selective pressures. Heavy metal resistance could play a role in the ability of the MRSA ChC to disseminate in specific geographical locations. [Image: see text] [Image: see text] DISCLOSURES: All authors: No reported disclosures. Oxford University Press 2019-10-23 /pmc/articles/PMC6811053/ http://dx.doi.org/10.1093/ofid/ofz360.625 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Abstracts
Martínez, Jose R W
Diaz, Lorena
Rojas, Marcelo
Rios, Rafael
Hanson, Blake
Rivas, Lina M
Spencer, Maria
Moustafa, Ahmed M
Araos Bralic, Rafael
Peters, Anne
Reyes, Jinnethe
Carvajal, Lina P
Luna, Carlos
Salles, Mauro
Alvarez, Carlos
Labarca, Jaime
Seas, Carlos
Seas, Carlos
Guzmán, Manuel
Planet, Paul J
Planet, Paul J
Arias, Cesar A
Munita, Jose
556. Phylogenomic Epidemiology of Methicillin-Resistant Staphylococcus aureus (MRSA) Chilean-Cordobes Clone in Latin America
title 556. Phylogenomic Epidemiology of Methicillin-Resistant Staphylococcus aureus (MRSA) Chilean-Cordobes Clone in Latin America
title_full 556. Phylogenomic Epidemiology of Methicillin-Resistant Staphylococcus aureus (MRSA) Chilean-Cordobes Clone in Latin America
title_fullStr 556. Phylogenomic Epidemiology of Methicillin-Resistant Staphylococcus aureus (MRSA) Chilean-Cordobes Clone in Latin America
title_full_unstemmed 556. Phylogenomic Epidemiology of Methicillin-Resistant Staphylococcus aureus (MRSA) Chilean-Cordobes Clone in Latin America
title_short 556. Phylogenomic Epidemiology of Methicillin-Resistant Staphylococcus aureus (MRSA) Chilean-Cordobes Clone in Latin America
title_sort 556. phylogenomic epidemiology of methicillin-resistant staphylococcus aureus (mrsa) chilean-cordobes clone in latin america
topic Abstracts
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6811053/
http://dx.doi.org/10.1093/ofid/ofz360.625
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