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The ant Lasius niger is a new source of bacterial enzymes with biotechnological potential for bleaching dye

Industrial synthetic dyes cause health and environmental problems. This work describes the isolation of 84 bacterial strains from the midgut of the Lasius niger ant and the evaluation of their potential application in dye bioremediation. Strains were identified and classified as judged by rRNA 16S....

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Autores principales: Díez-Méndez, Alexandra, García-Fraile, Paula, Solano, Francisco, Rivas, Raúl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6811527/
https://www.ncbi.nlm.nih.gov/pubmed/31645628
http://dx.doi.org/10.1038/s41598-019-51669-w
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author Díez-Méndez, Alexandra
García-Fraile, Paula
Solano, Francisco
Rivas, Raúl
author_facet Díez-Méndez, Alexandra
García-Fraile, Paula
Solano, Francisco
Rivas, Raúl
author_sort Díez-Méndez, Alexandra
collection PubMed
description Industrial synthetic dyes cause health and environmental problems. This work describes the isolation of 84 bacterial strains from the midgut of the Lasius niger ant and the evaluation of their potential application in dye bioremediation. Strains were identified and classified as judged by rRNA 16S. The most abundant isolates were found to belong to Actinobacteria (49%) and Firmicutes (47.2%). We analyzed the content in laccase, azoreductase and peroxidase activities and their ability to degrade three known dyes (azo, thiazine and anthraquinone) with different chemical structures. Strain Ln26 (identified as Brevibacterium permense) strongly decolorized the three dyes tested at different conditions. Strain Ln78 (Streptomyces ambofaciens) exhibited a high level of activity in the presence of Toluidine Blue (TB). It was determined that 8.5 was the optimal pH for these two strains, the optimal temperature conditions ranged between 22 and 37 °C, and acidic pHs and temperatures around 50 °C caused enzyme inactivation. Finally, the genome of the most promising candidate (Ln26, approximately 4.2 Mb in size) was sequenced. Genes coding for two DyP-type peroxidases, one laccase and one azoreductase were identified and account for the ability of this strain to effectively oxidize a variety of dyes with different chemical structures.
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spelling pubmed-68115272019-10-25 The ant Lasius niger is a new source of bacterial enzymes with biotechnological potential for bleaching dye Díez-Méndez, Alexandra García-Fraile, Paula Solano, Francisco Rivas, Raúl Sci Rep Article Industrial synthetic dyes cause health and environmental problems. This work describes the isolation of 84 bacterial strains from the midgut of the Lasius niger ant and the evaluation of their potential application in dye bioremediation. Strains were identified and classified as judged by rRNA 16S. The most abundant isolates were found to belong to Actinobacteria (49%) and Firmicutes (47.2%). We analyzed the content in laccase, azoreductase and peroxidase activities and their ability to degrade three known dyes (azo, thiazine and anthraquinone) with different chemical structures. Strain Ln26 (identified as Brevibacterium permense) strongly decolorized the three dyes tested at different conditions. Strain Ln78 (Streptomyces ambofaciens) exhibited a high level of activity in the presence of Toluidine Blue (TB). It was determined that 8.5 was the optimal pH for these two strains, the optimal temperature conditions ranged between 22 and 37 °C, and acidic pHs and temperatures around 50 °C caused enzyme inactivation. Finally, the genome of the most promising candidate (Ln26, approximately 4.2 Mb in size) was sequenced. Genes coding for two DyP-type peroxidases, one laccase and one azoreductase were identified and account for the ability of this strain to effectively oxidize a variety of dyes with different chemical structures. Nature Publishing Group UK 2019-10-23 /pmc/articles/PMC6811527/ /pubmed/31645628 http://dx.doi.org/10.1038/s41598-019-51669-w Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Díez-Méndez, Alexandra
García-Fraile, Paula
Solano, Francisco
Rivas, Raúl
The ant Lasius niger is a new source of bacterial enzymes with biotechnological potential for bleaching dye
title The ant Lasius niger is a new source of bacterial enzymes with biotechnological potential for bleaching dye
title_full The ant Lasius niger is a new source of bacterial enzymes with biotechnological potential for bleaching dye
title_fullStr The ant Lasius niger is a new source of bacterial enzymes with biotechnological potential for bleaching dye
title_full_unstemmed The ant Lasius niger is a new source of bacterial enzymes with biotechnological potential for bleaching dye
title_short The ant Lasius niger is a new source of bacterial enzymes with biotechnological potential for bleaching dye
title_sort ant lasius niger is a new source of bacterial enzymes with biotechnological potential for bleaching dye
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6811527/
https://www.ncbi.nlm.nih.gov/pubmed/31645628
http://dx.doi.org/10.1038/s41598-019-51669-w
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