Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation

Sensitivity of FASP was tested using SDS lysates from HeLa cells and mouse brain. Peptides were analyzed using a QExactive HF-X instrument. Whole cell lysates of Hela cells were processed with FASP using single or double, consecutive or successive, digestion with LysC or trypsin. The generated pepti...

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Autores principales: Wiśniewski, Jacek R., Zettl, Katharina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6811893/
https://www.ncbi.nlm.nih.gov/pubmed/31667293
http://dx.doi.org/10.1016/j.dib.2019.104530
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author Wiśniewski, Jacek R.
Zettl, Katharina
author_facet Wiśniewski, Jacek R.
Zettl, Katharina
author_sort Wiśniewski, Jacek R.
collection PubMed
description Sensitivity of FASP was tested using SDS lysates from HeLa cells and mouse brain. Peptides were analyzed using a QExactive HF-X instrument. Whole cell lysates of Hela cells were processed with FASP using single or double, consecutive or successive, digestion with LysC or trypsin. The generated peptides were analyzed using a LTQ-Orbitrap mass spectrometer. These datasets accompany “Filter Aided Sample Preparation – A Tutorial” (Wiśniewski, 2019).
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spelling pubmed-68118932019-10-30 Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation Wiśniewski, Jacek R. Zettl, Katharina Data Brief Chemistry Sensitivity of FASP was tested using SDS lysates from HeLa cells and mouse brain. Peptides were analyzed using a QExactive HF-X instrument. Whole cell lysates of Hela cells were processed with FASP using single or double, consecutive or successive, digestion with LysC or trypsin. The generated peptides were analyzed using a LTQ-Orbitrap mass spectrometer. These datasets accompany “Filter Aided Sample Preparation – A Tutorial” (Wiśniewski, 2019). Elsevier 2019-09-16 /pmc/articles/PMC6811893/ /pubmed/31667293 http://dx.doi.org/10.1016/j.dib.2019.104530 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Chemistry
Wiśniewski, Jacek R.
Zettl, Katharina
Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation
title Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation
title_full Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation
title_fullStr Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation
title_full_unstemmed Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation
title_short Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation
title_sort datasets: sensitivity and protein digestion course of proteomic filter aided sample preparation
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6811893/
https://www.ncbi.nlm.nih.gov/pubmed/31667293
http://dx.doi.org/10.1016/j.dib.2019.104530
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