Data on isolation and purification of fibrinolytic enzyme from Pseudomonas baetica SUHU25

The present dataset provides methodology to isolate and purify fibrinolytic enzyme from microbe isolated from the natural source. The information provided in this data article includes (1) isolation and identification of Pseudomonas baetica SUHU25, (2) optimization of cultural conditions, (3) extrac...

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Detalles Bibliográficos
Autores principales: Salunke, Asha S., Kharat, Arun S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Biochemistry, Genetics and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6811981/
https://www.ncbi.nlm.nih.gov/pubmed/31667215
http://dx.doi.org/10.1016/j.dib.2019.104369
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author Salunke, Asha S.
Kharat, Arun S.
author_facet Salunke, Asha S.
Kharat, Arun S.
author_sort Salunke, Asha S.
collection PubMed
description The present dataset provides methodology to isolate and purify fibrinolytic enzyme from microbe isolated from the natural source. The information provided in this data article includes (1) isolation and identification of Pseudomonas baetica SUHU25, (2) optimization of cultural conditions, (3) extraction and purification of fibrinolytic enzyme, (4) protein estimation, (5) assay of fibrinolytic activity, (6) SDS PAGE for purified enzyme protein, (7) effect of pH, temperature and metal ions on fibrinolytic activity of enzyme protein, and (8) In-vitro blood clot dissolution assay.
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spelling pubmed-68119812019-10-30 Data on isolation and purification of fibrinolytic enzyme from Pseudomonas baetica SUHU25 Salunke, Asha S. Kharat, Arun S. Data Brief Biochemistry, Genetics and Molecular Biology The present dataset provides methodology to isolate and purify fibrinolytic enzyme from microbe isolated from the natural source. The information provided in this data article includes (1) isolation and identification of Pseudomonas baetica SUHU25, (2) optimization of cultural conditions, (3) extraction and purification of fibrinolytic enzyme, (4) protein estimation, (5) assay of fibrinolytic activity, (6) SDS PAGE for purified enzyme protein, (7) effect of pH, temperature and metal ions on fibrinolytic activity of enzyme protein, and (8) In-vitro blood clot dissolution assay. Elsevier 2019-09-18 /pmc/articles/PMC6811981/ /pubmed/31667215 http://dx.doi.org/10.1016/j.dib.2019.104369 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Biochemistry, Genetics and Molecular Biology
Salunke, Asha S.
Kharat, Arun S.
Data on isolation and purification of fibrinolytic enzyme from Pseudomonas baetica SUHU25
title Data on isolation and purification of fibrinolytic enzyme from Pseudomonas baetica SUHU25
title_full Data on isolation and purification of fibrinolytic enzyme from Pseudomonas baetica SUHU25
title_fullStr Data on isolation and purification of fibrinolytic enzyme from Pseudomonas baetica SUHU25
title_full_unstemmed Data on isolation and purification of fibrinolytic enzyme from Pseudomonas baetica SUHU25
title_short Data on isolation and purification of fibrinolytic enzyme from Pseudomonas baetica SUHU25
title_sort data on isolation and purification of fibrinolytic enzyme from pseudomonas baetica suhu25
topic Biochemistry, Genetics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6811981/
https://www.ncbi.nlm.nih.gov/pubmed/31667215
http://dx.doi.org/10.1016/j.dib.2019.104369
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