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Contrast bath-induced neurite outgrowth in PC12m3 cells via the p38 mitogen-activated protein kinase pathway

We investigated the differentiation and activation of p38 MAPK induced by contrast bath in drug-hypersensitive PC12m3 mutant cells. The rate of neurite outgrowth in PC12m3 cells induced by contrast bath was much higher than that induced by warming or cooling alone or that induced by two warmings wit...

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Autores principales: Motoda, Hirotoshio, Hiragami, Fukumi, Kawamura, Kenji, Inoue, Shigeki, Gomita, Yutaka, Kano, Yoshio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6812228/
https://www.ncbi.nlm.nih.gov/pubmed/31667436
http://dx.doi.org/10.1016/j.heliyon.2019.e02656
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author Motoda, Hirotoshio
Hiragami, Fukumi
Kawamura, Kenji
Inoue, Shigeki
Gomita, Yutaka
Kano, Yoshio
author_facet Motoda, Hirotoshio
Hiragami, Fukumi
Kawamura, Kenji
Inoue, Shigeki
Gomita, Yutaka
Kano, Yoshio
author_sort Motoda, Hirotoshio
collection PubMed
description We investigated the differentiation and activation of p38 MAPK induced by contrast bath in drug-hypersensitive PC12m3 mutant cells. The rate of neurite outgrowth in PC12m3 cells induced by contrast bath was much higher than that induced by warming or cooling alone or that induced by two warmings with an interval of room temperature, indicating that contrast bath has a synergistic effect. The results of an experiment using a p38 MAPK inhibitor, SB203580, showed that neurite outgrowth of PC12m3 cells induced by contrast bath is p38 MAPK-dependent. Moreover, p38 MAPK activity induced by contrast bath was greater than that induced by warming or cooling alone, indicating that the synergistic effect of a contrast bath on neurite outgrowth depends on the activity of p38 MAPK. Since calcium ions are involved in the activations of P38 MAPK, we investigated the effect of the TRP ion channel inhibitor (Capsazepine) that inhibits calcium influx in the cells. Neurite outgrowth induced by contrast bath treatment was greatly suppressed by the addition of Capsazepine. These findings suggest that calcium dependent activation of the p38 MAPK pathway induced by contrast bath is responsible for the neurite outgrowth of PC12m3 cells.
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spelling pubmed-68122282019-10-30 Contrast bath-induced neurite outgrowth in PC12m3 cells via the p38 mitogen-activated protein kinase pathway Motoda, Hirotoshio Hiragami, Fukumi Kawamura, Kenji Inoue, Shigeki Gomita, Yutaka Kano, Yoshio Heliyon Article We investigated the differentiation and activation of p38 MAPK induced by contrast bath in drug-hypersensitive PC12m3 mutant cells. The rate of neurite outgrowth in PC12m3 cells induced by contrast bath was much higher than that induced by warming or cooling alone or that induced by two warmings with an interval of room temperature, indicating that contrast bath has a synergistic effect. The results of an experiment using a p38 MAPK inhibitor, SB203580, showed that neurite outgrowth of PC12m3 cells induced by contrast bath is p38 MAPK-dependent. Moreover, p38 MAPK activity induced by contrast bath was greater than that induced by warming or cooling alone, indicating that the synergistic effect of a contrast bath on neurite outgrowth depends on the activity of p38 MAPK. Since calcium ions are involved in the activations of P38 MAPK, we investigated the effect of the TRP ion channel inhibitor (Capsazepine) that inhibits calcium influx in the cells. Neurite outgrowth induced by contrast bath treatment was greatly suppressed by the addition of Capsazepine. These findings suggest that calcium dependent activation of the p38 MAPK pathway induced by contrast bath is responsible for the neurite outgrowth of PC12m3 cells. Elsevier 2019-10-15 /pmc/articles/PMC6812228/ /pubmed/31667436 http://dx.doi.org/10.1016/j.heliyon.2019.e02656 Text en © 2019 The Authors. Published by Elsevier Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Motoda, Hirotoshio
Hiragami, Fukumi
Kawamura, Kenji
Inoue, Shigeki
Gomita, Yutaka
Kano, Yoshio
Contrast bath-induced neurite outgrowth in PC12m3 cells via the p38 mitogen-activated protein kinase pathway
title Contrast bath-induced neurite outgrowth in PC12m3 cells via the p38 mitogen-activated protein kinase pathway
title_full Contrast bath-induced neurite outgrowth in PC12m3 cells via the p38 mitogen-activated protein kinase pathway
title_fullStr Contrast bath-induced neurite outgrowth in PC12m3 cells via the p38 mitogen-activated protein kinase pathway
title_full_unstemmed Contrast bath-induced neurite outgrowth in PC12m3 cells via the p38 mitogen-activated protein kinase pathway
title_short Contrast bath-induced neurite outgrowth in PC12m3 cells via the p38 mitogen-activated protein kinase pathway
title_sort contrast bath-induced neurite outgrowth in pc12m3 cells via the p38 mitogen-activated protein kinase pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6812228/
https://www.ncbi.nlm.nih.gov/pubmed/31667436
http://dx.doi.org/10.1016/j.heliyon.2019.e02656
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