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Determination of conjugated protein on nanoparticles by an adaptation of the Coomassie blue dye method

A new accurate spectrophotometric method for protein determination on nanoparticles is described. The method is based on the Coomassie blue dye that binds to the basic and aromatic amino acid residues of proteins, especially arginine and lysine. A known amount of reagent dye was mixed with a variety...

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Detalles Bibliográficos
Autores principales: Oviedo, Mariana J., Quester, Katrin, Hirata, Gustavo A., Vazquez-Duhalt, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6812394/
https://www.ncbi.nlm.nih.gov/pubmed/31667112
http://dx.doi.org/10.1016/j.mex.2019.09.015