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The Tudor SND1 protein is an m(6)A RNA reader essential for replication of Kaposi’s sarcoma-associated herpesvirus

N(6)-methyladenosine (m(6)A) is the most abundant internal RNA modification of cellular mRNAs. m(6)A is recognised by YTH domain-containing proteins, which selectively bind to m(6)A-decorated RNAs regulating their turnover and translation. Using an m(6)A-modified hairpin present in the Kaposi’s sarc...

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Autores principales: Baquero-Perez, Belinda, Antanaviciute, Agne, Yonchev, Ivaylo D, Carr, Ian M, Wilson, Stuart A, Whitehouse, Adrian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6812964/
https://www.ncbi.nlm.nih.gov/pubmed/31647415
http://dx.doi.org/10.7554/eLife.47261
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author Baquero-Perez, Belinda
Antanaviciute, Agne
Yonchev, Ivaylo D
Carr, Ian M
Wilson, Stuart A
Whitehouse, Adrian
author_facet Baquero-Perez, Belinda
Antanaviciute, Agne
Yonchev, Ivaylo D
Carr, Ian M
Wilson, Stuart A
Whitehouse, Adrian
author_sort Baquero-Perez, Belinda
collection PubMed
description N(6)-methyladenosine (m(6)A) is the most abundant internal RNA modification of cellular mRNAs. m(6)A is recognised by YTH domain-containing proteins, which selectively bind to m(6)A-decorated RNAs regulating their turnover and translation. Using an m(6)A-modified hairpin present in the Kaposi’s sarcoma associated herpesvirus (KSHV) ORF50 RNA, we identified seven members from the ‘Royal family’ as putative m(6)A readers, including SND1. RIP-seq and eCLIP analysis characterised the SND1 binding profile transcriptome-wide, revealing SND1 as an m(6)A reader. We further demonstrate that the m(6)A modification of the ORF50 RNA is critical for SND1 binding, which in turn stabilises the ORF50 transcript. Importantly, SND1 depletion leads to inhibition of KSHV early gene expression showing that SND1 is essential for KSHV lytic replication. This work demonstrates that members of the ‘Royal family’ have m(6)A-reading ability, greatly increasing their epigenetic functions beyond protein methylation.
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spelling pubmed-68129642019-10-25 The Tudor SND1 protein is an m(6)A RNA reader essential for replication of Kaposi’s sarcoma-associated herpesvirus Baquero-Perez, Belinda Antanaviciute, Agne Yonchev, Ivaylo D Carr, Ian M Wilson, Stuart A Whitehouse, Adrian eLife Microbiology and Infectious Disease N(6)-methyladenosine (m(6)A) is the most abundant internal RNA modification of cellular mRNAs. m(6)A is recognised by YTH domain-containing proteins, which selectively bind to m(6)A-decorated RNAs regulating their turnover and translation. Using an m(6)A-modified hairpin present in the Kaposi’s sarcoma associated herpesvirus (KSHV) ORF50 RNA, we identified seven members from the ‘Royal family’ as putative m(6)A readers, including SND1. RIP-seq and eCLIP analysis characterised the SND1 binding profile transcriptome-wide, revealing SND1 as an m(6)A reader. We further demonstrate that the m(6)A modification of the ORF50 RNA is critical for SND1 binding, which in turn stabilises the ORF50 transcript. Importantly, SND1 depletion leads to inhibition of KSHV early gene expression showing that SND1 is essential for KSHV lytic replication. This work demonstrates that members of the ‘Royal family’ have m(6)A-reading ability, greatly increasing their epigenetic functions beyond protein methylation. eLife Sciences Publications, Ltd 2019-10-24 /pmc/articles/PMC6812964/ /pubmed/31647415 http://dx.doi.org/10.7554/eLife.47261 Text en © 2019, Baquero-Perez et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Microbiology and Infectious Disease
Baquero-Perez, Belinda
Antanaviciute, Agne
Yonchev, Ivaylo D
Carr, Ian M
Wilson, Stuart A
Whitehouse, Adrian
The Tudor SND1 protein is an m(6)A RNA reader essential for replication of Kaposi’s sarcoma-associated herpesvirus
title The Tudor SND1 protein is an m(6)A RNA reader essential for replication of Kaposi’s sarcoma-associated herpesvirus
title_full The Tudor SND1 protein is an m(6)A RNA reader essential for replication of Kaposi’s sarcoma-associated herpesvirus
title_fullStr The Tudor SND1 protein is an m(6)A RNA reader essential for replication of Kaposi’s sarcoma-associated herpesvirus
title_full_unstemmed The Tudor SND1 protein is an m(6)A RNA reader essential for replication of Kaposi’s sarcoma-associated herpesvirus
title_short The Tudor SND1 protein is an m(6)A RNA reader essential for replication of Kaposi’s sarcoma-associated herpesvirus
title_sort tudor snd1 protein is an m(6)a rna reader essential for replication of kaposi’s sarcoma-associated herpesvirus
topic Microbiology and Infectious Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6812964/
https://www.ncbi.nlm.nih.gov/pubmed/31647415
http://dx.doi.org/10.7554/eLife.47261
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