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sli is required for proper morphology and migration of sensory neurons in the Drosophila PNS

Neurons and glial cells coordinate with each other in many different aspects of nervous system development. Both types of cells are receiving multiple guidance cues to guide the neurons and glial cells to their proper final position. The lateral chordotonal organs (lch5) of the Drosophila peripheral...

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Autores principales: Gonsior, Madison, Ismat, Afshan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6813078/
https://www.ncbi.nlm.nih.gov/pubmed/31651354
http://dx.doi.org/10.1186/s13064-019-0135-z
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author Gonsior, Madison
Ismat, Afshan
author_facet Gonsior, Madison
Ismat, Afshan
author_sort Gonsior, Madison
collection PubMed
description Neurons and glial cells coordinate with each other in many different aspects of nervous system development. Both types of cells are receiving multiple guidance cues to guide the neurons and glial cells to their proper final position. The lateral chordotonal organs (lch5) of the Drosophila peripheral nervous system (PNS) are composed of five sensory neurons surrounded by four different glial cells, scolopale cells, cap cells, attachment cells and ligament cells. During embryogenesis, the lch5 neurons go through a rotation and ventral migration to reach their final position in the lateral region of the abdomen. We show here that the extracellular ligand sli is required for the proper ventral migration and morphology of the lch5 neurons. We further show that mutations in the Sli receptors Robo and Robo2 also display similar defects as loss of sli, suggesting a role for Slit-Robo signaling in lch5 migration and positioning. Additionally, we demonstrate that the scolopale, cap and attachment cells follow the mis-migrated lch5 neurons in sli mutants, while the ventral stretching of the ligament cells seems to be independent of the lch5 neurons. This study sheds light on the role of Slit-Robo signaling in sensory neuron development.
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spelling pubmed-68130782019-10-30 sli is required for proper morphology and migration of sensory neurons in the Drosophila PNS Gonsior, Madison Ismat, Afshan Neural Dev Short Report Neurons and glial cells coordinate with each other in many different aspects of nervous system development. Both types of cells are receiving multiple guidance cues to guide the neurons and glial cells to their proper final position. The lateral chordotonal organs (lch5) of the Drosophila peripheral nervous system (PNS) are composed of five sensory neurons surrounded by four different glial cells, scolopale cells, cap cells, attachment cells and ligament cells. During embryogenesis, the lch5 neurons go through a rotation and ventral migration to reach their final position in the lateral region of the abdomen. We show here that the extracellular ligand sli is required for the proper ventral migration and morphology of the lch5 neurons. We further show that mutations in the Sli receptors Robo and Robo2 also display similar defects as loss of sli, suggesting a role for Slit-Robo signaling in lch5 migration and positioning. Additionally, we demonstrate that the scolopale, cap and attachment cells follow the mis-migrated lch5 neurons in sli mutants, while the ventral stretching of the ligament cells seems to be independent of the lch5 neurons. This study sheds light on the role of Slit-Robo signaling in sensory neuron development. BioMed Central 2019-10-24 /pmc/articles/PMC6813078/ /pubmed/31651354 http://dx.doi.org/10.1186/s13064-019-0135-z Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Gonsior, Madison
Ismat, Afshan
sli is required for proper morphology and migration of sensory neurons in the Drosophila PNS
title sli is required for proper morphology and migration of sensory neurons in the Drosophila PNS
title_full sli is required for proper morphology and migration of sensory neurons in the Drosophila PNS
title_fullStr sli is required for proper morphology and migration of sensory neurons in the Drosophila PNS
title_full_unstemmed sli is required for proper morphology and migration of sensory neurons in the Drosophila PNS
title_short sli is required for proper morphology and migration of sensory neurons in the Drosophila PNS
title_sort sli is required for proper morphology and migration of sensory neurons in the drosophila pns
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6813078/
https://www.ncbi.nlm.nih.gov/pubmed/31651354
http://dx.doi.org/10.1186/s13064-019-0135-z
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