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Evaluation of the anti-cancer potential of Cedrus deodara total lignans by inducing apoptosis of A549 cells
BACKGROUND: Cedrus deodara (Roxb.) Loud (normally called as deodar), one out of four species in the genus Cedrus, exhibits widely biological activities. The Cedrus deodara total lignans from the pine needles (CTL) were extracted. The aim of the study was to investigate the anticancer potential of th...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6813103/ https://www.ncbi.nlm.nih.gov/pubmed/31651320 http://dx.doi.org/10.1186/s12906-019-2682-6 |
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author | Shi, Xiaofeng Du, Ruiqin Zhang, Junmin Lei, Yanping Guo, Hongyun |
author_facet | Shi, Xiaofeng Du, Ruiqin Zhang, Junmin Lei, Yanping Guo, Hongyun |
author_sort | Shi, Xiaofeng |
collection | PubMed |
description | BACKGROUND: Cedrus deodara (Roxb.) Loud (normally called as deodar), one out of four species in the genus Cedrus, exhibits widely biological activities. The Cedrus deodara total lignans from the pine needles (CTL) were extracted. The aim of the study was to investigate the anticancer potential of the CTL on A549 cell line. METHODS: We extracted the CTL by ethanol and assessed the cytotoxicity by CCK-8 method. Cell cycle and apoptosis were detected by a FACS Verse Calibur flow cytometry. RESULTS: The CTL were extracted by means of ethanol hot refluxing and the content of total lignans in CTL was about 55.77%. By the CCK-8 assays, CTL inhibited the growth of A549 cells in a dose-dependent fashion, with the IC(50) values of 39.82 ± 1.74 μg/mL. CTL also inhibited the growth to a less extent in HeLa, HepG2, MKN28 and HT-29 cells. CONCLUSION: At low doses, the CTL effectively inhibited the growth of A549 cells. By comparison of IC(50) values, we found that A549 cells might be more sensitive to the treatment with CTL. In addition, CTL were also able to increase the population of A549 cells in G2/M phase and the percentage of apoptotic A549 cells. CTL may have therapeutic potential in lung adenocarcinoma cancer by regulating cell cycle and apoptosis. |
format | Online Article Text |
id | pubmed-6813103 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-68131032019-10-30 Evaluation of the anti-cancer potential of Cedrus deodara total lignans by inducing apoptosis of A549 cells Shi, Xiaofeng Du, Ruiqin Zhang, Junmin Lei, Yanping Guo, Hongyun BMC Complement Altern Med Research Article BACKGROUND: Cedrus deodara (Roxb.) Loud (normally called as deodar), one out of four species in the genus Cedrus, exhibits widely biological activities. The Cedrus deodara total lignans from the pine needles (CTL) were extracted. The aim of the study was to investigate the anticancer potential of the CTL on A549 cell line. METHODS: We extracted the CTL by ethanol and assessed the cytotoxicity by CCK-8 method. Cell cycle and apoptosis were detected by a FACS Verse Calibur flow cytometry. RESULTS: The CTL were extracted by means of ethanol hot refluxing and the content of total lignans in CTL was about 55.77%. By the CCK-8 assays, CTL inhibited the growth of A549 cells in a dose-dependent fashion, with the IC(50) values of 39.82 ± 1.74 μg/mL. CTL also inhibited the growth to a less extent in HeLa, HepG2, MKN28 and HT-29 cells. CONCLUSION: At low doses, the CTL effectively inhibited the growth of A549 cells. By comparison of IC(50) values, we found that A549 cells might be more sensitive to the treatment with CTL. In addition, CTL were also able to increase the population of A549 cells in G2/M phase and the percentage of apoptotic A549 cells. CTL may have therapeutic potential in lung adenocarcinoma cancer by regulating cell cycle and apoptosis. BioMed Central 2019-10-24 /pmc/articles/PMC6813103/ /pubmed/31651320 http://dx.doi.org/10.1186/s12906-019-2682-6 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Shi, Xiaofeng Du, Ruiqin Zhang, Junmin Lei, Yanping Guo, Hongyun Evaluation of the anti-cancer potential of Cedrus deodara total lignans by inducing apoptosis of A549 cells |
title | Evaluation of the anti-cancer potential of Cedrus deodara total lignans by inducing apoptosis of A549 cells |
title_full | Evaluation of the anti-cancer potential of Cedrus deodara total lignans by inducing apoptosis of A549 cells |
title_fullStr | Evaluation of the anti-cancer potential of Cedrus deodara total lignans by inducing apoptosis of A549 cells |
title_full_unstemmed | Evaluation of the anti-cancer potential of Cedrus deodara total lignans by inducing apoptosis of A549 cells |
title_short | Evaluation of the anti-cancer potential of Cedrus deodara total lignans by inducing apoptosis of A549 cells |
title_sort | evaluation of the anti-cancer potential of cedrus deodara total lignans by inducing apoptosis of a549 cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6813103/ https://www.ncbi.nlm.nih.gov/pubmed/31651320 http://dx.doi.org/10.1186/s12906-019-2682-6 |
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