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Identification of Gut Microbiota and Metabolites Signature in Patients With Irritable Bowel Syndrome

Background and Aims: Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder. However, the underlying mechanism of IBS is not fully understood. The aim of this study was to investigate potential mechanism and novel biomarkers of IBS through evaluation of the metabolomic and m...

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Autores principales: Zhu, Shengtao, Liu, Si, Li, Hengcun, Zhang, Zheng, Zhang, Qian, Chen, Lei, Zhao, Yu, Chen, Yang, Gu, Junchao, Min, Li, Zhang, Shutian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6813219/
https://www.ncbi.nlm.nih.gov/pubmed/31681624
http://dx.doi.org/10.3389/fcimb.2019.00346
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author Zhu, Shengtao
Liu, Si
Li, Hengcun
Zhang, Zheng
Zhang, Qian
Chen, Lei
Zhao, Yu
Chen, Yang
Gu, Junchao
Min, Li
Zhang, Shutian
author_facet Zhu, Shengtao
Liu, Si
Li, Hengcun
Zhang, Zheng
Zhang, Qian
Chen, Lei
Zhao, Yu
Chen, Yang
Gu, Junchao
Min, Li
Zhang, Shutian
author_sort Zhu, Shengtao
collection PubMed
description Background and Aims: Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder. However, the underlying mechanism of IBS is not fully understood. The aim of this study was to investigate potential mechanism and novel biomarkers of IBS through evaluation of the metabolomic and microbiologic profile. Methods: Fecal samples were collected from 15 irritable bowel syndrome patients and 15 healthy controls. By using gas chromatography coupled to time-of-flight mass spectrometry (GC-TOFMS) and 16S rDNA amplicon sequencing, fecal metabolites and microbiota of healthy controls and the IBS patients were measured. Results: IBS patients had a significantly differential metabolite profile as compared to healthy controls, and 4 clusters with 31 metabolites, including a group of amino acids and fatty acids, were significantly up-regulated as compared to the healthy controls. In addition, 19 microbes were significantly up-regulated, and 12 microbes were down-regulated in the IBS group, when compared with the healthy controls. Some clusters of fecal metabolites or microorganisms were significantly correlated with the severity of IBS symptoms, such as the frequency of abdominal pain/discomfort and the number of bowel movements. Correlation of the metabolite levels with abundances of microbial genera showed some statistically significant metabolite-microbe associations. Four differentially abundant amino acids clustered together were positively correlated with some microbes, including Lachnospira, Clostridium, and so on. Conclusion: The finding of this study puts a global perspective on metabolomics and microbiota profiling in IBS patients and provides a theoretical basis for future research on pathophysiology of IBS.
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spelling pubmed-68132192019-11-01 Identification of Gut Microbiota and Metabolites Signature in Patients With Irritable Bowel Syndrome Zhu, Shengtao Liu, Si Li, Hengcun Zhang, Zheng Zhang, Qian Chen, Lei Zhao, Yu Chen, Yang Gu, Junchao Min, Li Zhang, Shutian Front Cell Infect Microbiol Cellular and Infection Microbiology Background and Aims: Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder. However, the underlying mechanism of IBS is not fully understood. The aim of this study was to investigate potential mechanism and novel biomarkers of IBS through evaluation of the metabolomic and microbiologic profile. Methods: Fecal samples were collected from 15 irritable bowel syndrome patients and 15 healthy controls. By using gas chromatography coupled to time-of-flight mass spectrometry (GC-TOFMS) and 16S rDNA amplicon sequencing, fecal metabolites and microbiota of healthy controls and the IBS patients were measured. Results: IBS patients had a significantly differential metabolite profile as compared to healthy controls, and 4 clusters with 31 metabolites, including a group of amino acids and fatty acids, were significantly up-regulated as compared to the healthy controls. In addition, 19 microbes were significantly up-regulated, and 12 microbes were down-regulated in the IBS group, when compared with the healthy controls. Some clusters of fecal metabolites or microorganisms were significantly correlated with the severity of IBS symptoms, such as the frequency of abdominal pain/discomfort and the number of bowel movements. Correlation of the metabolite levels with abundances of microbial genera showed some statistically significant metabolite-microbe associations. Four differentially abundant amino acids clustered together were positively correlated with some microbes, including Lachnospira, Clostridium, and so on. Conclusion: The finding of this study puts a global perspective on metabolomics and microbiota profiling in IBS patients and provides a theoretical basis for future research on pathophysiology of IBS. Frontiers Media S.A. 2019-10-18 /pmc/articles/PMC6813219/ /pubmed/31681624 http://dx.doi.org/10.3389/fcimb.2019.00346 Text en Copyright © 2019 Zhu, Liu, Li, Zhang, Zhang, Chen, Zhao, Chen, Gu, Min and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Zhu, Shengtao
Liu, Si
Li, Hengcun
Zhang, Zheng
Zhang, Qian
Chen, Lei
Zhao, Yu
Chen, Yang
Gu, Junchao
Min, Li
Zhang, Shutian
Identification of Gut Microbiota and Metabolites Signature in Patients With Irritable Bowel Syndrome
title Identification of Gut Microbiota and Metabolites Signature in Patients With Irritable Bowel Syndrome
title_full Identification of Gut Microbiota and Metabolites Signature in Patients With Irritable Bowel Syndrome
title_fullStr Identification of Gut Microbiota and Metabolites Signature in Patients With Irritable Bowel Syndrome
title_full_unstemmed Identification of Gut Microbiota and Metabolites Signature in Patients With Irritable Bowel Syndrome
title_short Identification of Gut Microbiota and Metabolites Signature in Patients With Irritable Bowel Syndrome
title_sort identification of gut microbiota and metabolites signature in patients with irritable bowel syndrome
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6813219/
https://www.ncbi.nlm.nih.gov/pubmed/31681624
http://dx.doi.org/10.3389/fcimb.2019.00346
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