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Anticancer activities of toxic isolate of Xestospongia testudinaria sponge
AIMS: The purposes of this study were to determine the anticancer activity of Xestospongia testudinaria sponge isolate and identify the responsible compounds. MATERIALS AND METHODS: The metabolites were extracted using methanol maceration at room temperature. The separation and purification of metab...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6813599/ https://www.ncbi.nlm.nih.gov/pubmed/31749578 http://dx.doi.org/10.14202/vetworld.2019.1434-1440 |
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author | Swantara, Made Dira Rita, Wiwik Susanah Suartha, Nyoman Agustina, Kadek Karang |
author_facet | Swantara, Made Dira Rita, Wiwik Susanah Suartha, Nyoman Agustina, Kadek Karang |
author_sort | Swantara, Made Dira |
collection | PubMed |
description | AIMS: The purposes of this study were to determine the anticancer activity of Xestospongia testudinaria sponge isolate and identify the responsible compounds. MATERIALS AND METHODS: The metabolites were extracted using methanol maceration at room temperature. The separation and purification of metabolites were performed using fractionation and column chromatography. The toxicity was examined using the brine shrimp lethality assay, and the toxic isolates were tested for anticancer activity against HeLa cells. Gas chromatography-mass spectrometry analysis was used to identify the compounds in the isolate. RESULTS: When the methanol extract was partitioned with n-hexane, chloroform, and n-butanol, the chloroform fraction was the most toxic, with a concentration that results in 50% lethality (LC(50)) value of 39.81 ppm. After separation of the chloroform extract, fraction B (FB) was the most toxic, with an LC(50) value of 44.67 ppm. The isolate from FB showed anticancer activity with a concentration at which 50% of growth was inhibited (IC(50)) of 2.273 ppm. In total, 21 compounds were identified in anticancer isolates: Nonanedioic acid; tetradecanoic acid; trans-phytol; 2-pentadecanone-6,10,14-trimethyl; pentadecanoic acid; 2-hexadecen-1-ol, 3,7,11,15-tetramethyl-; pentadecanoic acid; 2-hexadecen-1-ol, 3,7,11,15-tetramethyl-; 2,3,7-trimethyloctanal; palmitic acid; docosanoic acid, ethyl ester; 1,E-11,Z-13-octadecatriene; chloromethyl 4-chlorododecanoate; 1-tricosene; 9,12-octadecadienoic acid; 4,8,12,16-tetramethylheptadecan-4-olide; 1-docosene; heneicosane; phosphonic acid, dioctadecyl ester; dodecane,4,6-dimethyl-; n-tetratriacontane; 1-iodohexadecane; and n-heneicosane. CONCLUSION: These findings indicate that the isolate of X. testudinaria can be used as a natural anticancer toward HeLa cell. |
format | Online Article Text |
id | pubmed-6813599 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-68135992019-11-20 Anticancer activities of toxic isolate of Xestospongia testudinaria sponge Swantara, Made Dira Rita, Wiwik Susanah Suartha, Nyoman Agustina, Kadek Karang Vet World Research Article AIMS: The purposes of this study were to determine the anticancer activity of Xestospongia testudinaria sponge isolate and identify the responsible compounds. MATERIALS AND METHODS: The metabolites were extracted using methanol maceration at room temperature. The separation and purification of metabolites were performed using fractionation and column chromatography. The toxicity was examined using the brine shrimp lethality assay, and the toxic isolates were tested for anticancer activity against HeLa cells. Gas chromatography-mass spectrometry analysis was used to identify the compounds in the isolate. RESULTS: When the methanol extract was partitioned with n-hexane, chloroform, and n-butanol, the chloroform fraction was the most toxic, with a concentration that results in 50% lethality (LC(50)) value of 39.81 ppm. After separation of the chloroform extract, fraction B (FB) was the most toxic, with an LC(50) value of 44.67 ppm. The isolate from FB showed anticancer activity with a concentration at which 50% of growth was inhibited (IC(50)) of 2.273 ppm. In total, 21 compounds were identified in anticancer isolates: Nonanedioic acid; tetradecanoic acid; trans-phytol; 2-pentadecanone-6,10,14-trimethyl; pentadecanoic acid; 2-hexadecen-1-ol, 3,7,11,15-tetramethyl-; pentadecanoic acid; 2-hexadecen-1-ol, 3,7,11,15-tetramethyl-; 2,3,7-trimethyloctanal; palmitic acid; docosanoic acid, ethyl ester; 1,E-11,Z-13-octadecatriene; chloromethyl 4-chlorododecanoate; 1-tricosene; 9,12-octadecadienoic acid; 4,8,12,16-tetramethylheptadecan-4-olide; 1-docosene; heneicosane; phosphonic acid, dioctadecyl ester; dodecane,4,6-dimethyl-; n-tetratriacontane; 1-iodohexadecane; and n-heneicosane. CONCLUSION: These findings indicate that the isolate of X. testudinaria can be used as a natural anticancer toward HeLa cell. Veterinary World 2019-09 /pmc/articles/PMC6813599/ /pubmed/31749578 http://dx.doi.org/10.14202/vetworld.2019.1434-1440 Text en Copyright: © Swantara, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Swantara, Made Dira Rita, Wiwik Susanah Suartha, Nyoman Agustina, Kadek Karang Anticancer activities of toxic isolate of Xestospongia testudinaria sponge |
title | Anticancer activities of toxic isolate of Xestospongia testudinaria sponge |
title_full | Anticancer activities of toxic isolate of Xestospongia testudinaria sponge |
title_fullStr | Anticancer activities of toxic isolate of Xestospongia testudinaria sponge |
title_full_unstemmed | Anticancer activities of toxic isolate of Xestospongia testudinaria sponge |
title_short | Anticancer activities of toxic isolate of Xestospongia testudinaria sponge |
title_sort | anticancer activities of toxic isolate of xestospongia testudinaria sponge |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6813599/ https://www.ncbi.nlm.nih.gov/pubmed/31749578 http://dx.doi.org/10.14202/vetworld.2019.1434-1440 |
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