An optimised CRISPR/Cas9 protocol to create targeted mutations in homoeologous genes and an efficient genotyping protocol to identify edited events in wheat

BACKGROUND: Targeted genome editing using the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has been applied in a large number of plant species. Using a gene-specific single guide RNA (sgRNA) and the CRISPR/Cas9 system, small editing events such as deletions of few b...

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Detalles Bibliográficos
Autores principales: Cui, Xiucheng, Balcerzak, Margaret, Schernthaner, Johann, Babic, Vivijan, Datla, Raju, Brauer, Elizabeth K., Labbé, Natalie, Subramaniam, Rajagopal, Ouellet, Thérèse
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814032/
https://www.ncbi.nlm.nih.gov/pubmed/31673276
http://dx.doi.org/10.1186/s13007-019-0500-2

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