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Biofilm production of coagulase-negative staphylococci isolated from rescued wild animals in the Republic of Korea
Biofilm production is a well-known causative factor of catheter- and medical device-related sepsis. Its high prevalence in coagulase-negative staphylococci (CoNS) has recently been reported. Information on biofilm production in CoNS isolated from wild animals is lacking. Herein, we studied the biofi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814046/ https://www.ncbi.nlm.nih.gov/pubmed/31651349 http://dx.doi.org/10.1186/s13028-019-0485-x |
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author | Lee, Sangjun Hwang, Jehwi Kim, Jongwoon Lee, Joonyeop Kim, Hong-Cheul Rhim, Haerin Han, Jae-Ik |
author_facet | Lee, Sangjun Hwang, Jehwi Kim, Jongwoon Lee, Joonyeop Kim, Hong-Cheul Rhim, Haerin Han, Jae-Ik |
author_sort | Lee, Sangjun |
collection | PubMed |
description | Biofilm production is a well-known causative factor of catheter- and medical device-related sepsis. Its high prevalence in coagulase-negative staphylococci (CoNS) has recently been reported. Information on biofilm production in CoNS isolated from wild animals is lacking. Herein, we studied the biofilm formation capabilities of CoNS isolated from rescued wild animals in the Republic of Korea. Swab samples were collected from the conjunctiva, nasal cavity, perianal area, and rectum for mammals while the sampling was done from the conjunctiva, oral mucosa, pericloacal area, and cloaca for birds. Isolation of CoNS was based on morphological and biochemical analyses along with molecular typing. Biofilm production was analyzed using 96-well plate based quantitative adherence assays. The studies demonstrated that CoNS of mammalian origin have higher biofilm-producing ability (70.4%) than the isolates from birds (62.5%). In particular, all methicillin-resistant (MR) CoNS isolated from mammals were capable of biofilm formation while only 63.3% of MR CoNS isolated from birds could produce biofilms. The MR CoNS isolated from mammals also had a significantly higher ability to form biofilms (100%) than methicillin susceptible CoNS (60.0%) than those isolates from birds. The findings show that wild animals may act as reservoirs as well as possible transmitters of biofilm-mediated antibiotic resistant genes. |
format | Online Article Text |
id | pubmed-6814046 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-68140462019-10-31 Biofilm production of coagulase-negative staphylococci isolated from rescued wild animals in the Republic of Korea Lee, Sangjun Hwang, Jehwi Kim, Jongwoon Lee, Joonyeop Kim, Hong-Cheul Rhim, Haerin Han, Jae-Ik Acta Vet Scand Brief Communication Biofilm production is a well-known causative factor of catheter- and medical device-related sepsis. Its high prevalence in coagulase-negative staphylococci (CoNS) has recently been reported. Information on biofilm production in CoNS isolated from wild animals is lacking. Herein, we studied the biofilm formation capabilities of CoNS isolated from rescued wild animals in the Republic of Korea. Swab samples were collected from the conjunctiva, nasal cavity, perianal area, and rectum for mammals while the sampling was done from the conjunctiva, oral mucosa, pericloacal area, and cloaca for birds. Isolation of CoNS was based on morphological and biochemical analyses along with molecular typing. Biofilm production was analyzed using 96-well plate based quantitative adherence assays. The studies demonstrated that CoNS of mammalian origin have higher biofilm-producing ability (70.4%) than the isolates from birds (62.5%). In particular, all methicillin-resistant (MR) CoNS isolated from mammals were capable of biofilm formation while only 63.3% of MR CoNS isolated from birds could produce biofilms. The MR CoNS isolated from mammals also had a significantly higher ability to form biofilms (100%) than methicillin susceptible CoNS (60.0%) than those isolates from birds. The findings show that wild animals may act as reservoirs as well as possible transmitters of biofilm-mediated antibiotic resistant genes. BioMed Central 2019-10-24 /pmc/articles/PMC6814046/ /pubmed/31651349 http://dx.doi.org/10.1186/s13028-019-0485-x Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Brief Communication Lee, Sangjun Hwang, Jehwi Kim, Jongwoon Lee, Joonyeop Kim, Hong-Cheul Rhim, Haerin Han, Jae-Ik Biofilm production of coagulase-negative staphylococci isolated from rescued wild animals in the Republic of Korea |
title | Biofilm production of coagulase-negative staphylococci isolated from rescued wild animals in the Republic of Korea |
title_full | Biofilm production of coagulase-negative staphylococci isolated from rescued wild animals in the Republic of Korea |
title_fullStr | Biofilm production of coagulase-negative staphylococci isolated from rescued wild animals in the Republic of Korea |
title_full_unstemmed | Biofilm production of coagulase-negative staphylococci isolated from rescued wild animals in the Republic of Korea |
title_short | Biofilm production of coagulase-negative staphylococci isolated from rescued wild animals in the Republic of Korea |
title_sort | biofilm production of coagulase-negative staphylococci isolated from rescued wild animals in the republic of korea |
topic | Brief Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814046/ https://www.ncbi.nlm.nih.gov/pubmed/31651349 http://dx.doi.org/10.1186/s13028-019-0485-x |
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