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Protocol for rapid clearing and staining of fixed Arabidopsis ovules for improved imaging by confocal laser scanning microscopy

BACKGROUND: A salient topic in developmental biology relates to the molecular and genetic mechanisms that underlie tissue morphogenesis. Modern quantitative approaches to this central question frequently involve digital cellular models of the organ or tissue under study. The ovules of the model spec...

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Detalles Bibliográficos
Autores principales: Tofanelli, Rachele, Vijayan, Athul, Scholz, Sebastian, Schneitz, Kay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814113/
https://www.ncbi.nlm.nih.gov/pubmed/31673277
http://dx.doi.org/10.1186/s13007-019-0505-x