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Protocol for rapid clearing and staining of fixed Arabidopsis ovules for improved imaging by confocal laser scanning microscopy
BACKGROUND: A salient topic in developmental biology relates to the molecular and genetic mechanisms that underlie tissue morphogenesis. Modern quantitative approaches to this central question frequently involve digital cellular models of the organ or tissue under study. The ovules of the model spec...
Autores principales: | Tofanelli, Rachele, Vijayan, Athul, Scholz, Sebastian, Schneitz, Kay |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814113/ https://www.ncbi.nlm.nih.gov/pubmed/31673277 http://dx.doi.org/10.1186/s13007-019-0505-x |
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