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Synthesis of Gold Nanoparticles Using Mimosa tenuiflora Extract, Assessments of Cytotoxicity, Cellular Uptake, and Catalysis

Synthesis of gold nanoparticles (AuNPs) with plant extracts has gained great interest in the field of biomedicine due to its wide variety of health applications. In the present work, AuNPs were synthesized with Mimosa tenuiflora (Mt) bark extract at different metallic precursor concentrations. Mt ex...

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Autores principales: Rodríguez-León, Ericka, Rodríguez-Vázquez, Blanca E., Martínez-Higuera, Aarón, Rodríguez-Beas, César, Larios-Rodríguez, Eduardo, Navarro, Rosa E., López-Esparza, Ricardo, Iñiguez-Palomares, Ramón A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814701/
https://www.ncbi.nlm.nih.gov/pubmed/31654146
http://dx.doi.org/10.1186/s11671-019-3158-9
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author Rodríguez-León, Ericka
Rodríguez-Vázquez, Blanca E.
Martínez-Higuera, Aarón
Rodríguez-Beas, César
Larios-Rodríguez, Eduardo
Navarro, Rosa E.
López-Esparza, Ricardo
Iñiguez-Palomares, Ramón A.
author_facet Rodríguez-León, Ericka
Rodríguez-Vázquez, Blanca E.
Martínez-Higuera, Aarón
Rodríguez-Beas, César
Larios-Rodríguez, Eduardo
Navarro, Rosa E.
López-Esparza, Ricardo
Iñiguez-Palomares, Ramón A.
author_sort Rodríguez-León, Ericka
collection PubMed
description Synthesis of gold nanoparticles (AuNPs) with plant extracts has gained great interest in the field of biomedicine due to its wide variety of health applications. In the present work, AuNPs were synthesized with Mimosa tenuiflora (Mt) bark extract at different metallic precursor concentrations. Mt extract was obtained by mixing the tree bark in ethanol-water. The antioxidant capacity of extract was evaluated using 2,2-diphenyl-1-picrylhydrazyl and total polyphenol assay. AuNPs were characterized by transmission electron microscopy, X-ray diffraction, UV-Vis and Fourier transform infrared spectroscopy, and X-ray photoelectron spectrometry for functional group determination onto their surface. AuMt (colloids formed by AuNPs and molecules of Mt) exhibit multiple shapes with sizes between 20 and 200 nm. AuMt were tested on methylene blue degradation in homogeneous catalysis adding sodium borohydride. The smallest NPs (AuMt1) have a degradation coefficient of 0.008/s and reach 50% degradation in 190s. Cell viability and cytotoxicity were evaluated in human umbilical vein endothelial cells (HUVEC), and a moderate cytotoxic effect at 24 and 48 h was found. However, toxicity does not behave in a dose-dependent manner. Cellular internalization of AuMt on HUVEC cells was analyzed by confocal laser scanning microscopy. For AuMt1, it can be observed that the material is dispersed into the cytoplasm, while in AuMt2, the material is concentrated in the nuclear periphery.
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spelling pubmed-68147012019-11-08 Synthesis of Gold Nanoparticles Using Mimosa tenuiflora Extract, Assessments of Cytotoxicity, Cellular Uptake, and Catalysis Rodríguez-León, Ericka Rodríguez-Vázquez, Blanca E. Martínez-Higuera, Aarón Rodríguez-Beas, César Larios-Rodríguez, Eduardo Navarro, Rosa E. López-Esparza, Ricardo Iñiguez-Palomares, Ramón A. Nanoscale Res Lett Nano Express Synthesis of gold nanoparticles (AuNPs) with plant extracts has gained great interest in the field of biomedicine due to its wide variety of health applications. In the present work, AuNPs were synthesized with Mimosa tenuiflora (Mt) bark extract at different metallic precursor concentrations. Mt extract was obtained by mixing the tree bark in ethanol-water. The antioxidant capacity of extract was evaluated using 2,2-diphenyl-1-picrylhydrazyl and total polyphenol assay. AuNPs were characterized by transmission electron microscopy, X-ray diffraction, UV-Vis and Fourier transform infrared spectroscopy, and X-ray photoelectron spectrometry for functional group determination onto their surface. AuMt (colloids formed by AuNPs and molecules of Mt) exhibit multiple shapes with sizes between 20 and 200 nm. AuMt were tested on methylene blue degradation in homogeneous catalysis adding sodium borohydride. The smallest NPs (AuMt1) have a degradation coefficient of 0.008/s and reach 50% degradation in 190s. Cell viability and cytotoxicity were evaluated in human umbilical vein endothelial cells (HUVEC), and a moderate cytotoxic effect at 24 and 48 h was found. However, toxicity does not behave in a dose-dependent manner. Cellular internalization of AuMt on HUVEC cells was analyzed by confocal laser scanning microscopy. For AuMt1, it can be observed that the material is dispersed into the cytoplasm, while in AuMt2, the material is concentrated in the nuclear periphery. Springer US 2019-10-26 /pmc/articles/PMC6814701/ /pubmed/31654146 http://dx.doi.org/10.1186/s11671-019-3158-9 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Nano Express
Rodríguez-León, Ericka
Rodríguez-Vázquez, Blanca E.
Martínez-Higuera, Aarón
Rodríguez-Beas, César
Larios-Rodríguez, Eduardo
Navarro, Rosa E.
López-Esparza, Ricardo
Iñiguez-Palomares, Ramón A.
Synthesis of Gold Nanoparticles Using Mimosa tenuiflora Extract, Assessments of Cytotoxicity, Cellular Uptake, and Catalysis
title Synthesis of Gold Nanoparticles Using Mimosa tenuiflora Extract, Assessments of Cytotoxicity, Cellular Uptake, and Catalysis
title_full Synthesis of Gold Nanoparticles Using Mimosa tenuiflora Extract, Assessments of Cytotoxicity, Cellular Uptake, and Catalysis
title_fullStr Synthesis of Gold Nanoparticles Using Mimosa tenuiflora Extract, Assessments of Cytotoxicity, Cellular Uptake, and Catalysis
title_full_unstemmed Synthesis of Gold Nanoparticles Using Mimosa tenuiflora Extract, Assessments of Cytotoxicity, Cellular Uptake, and Catalysis
title_short Synthesis of Gold Nanoparticles Using Mimosa tenuiflora Extract, Assessments of Cytotoxicity, Cellular Uptake, and Catalysis
title_sort synthesis of gold nanoparticles using mimosa tenuiflora extract, assessments of cytotoxicity, cellular uptake, and catalysis
topic Nano Express
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814701/
https://www.ncbi.nlm.nih.gov/pubmed/31654146
http://dx.doi.org/10.1186/s11671-019-3158-9
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