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CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner
The fine-tuning of gene expression contributes to both basic science and applications. Here, we develop a novel gene expression technology termed CRITGI (CRISPR/Transposon gene integration). CRITGI uses CRISPR/Cas9 to integrate multiple copies of the plasmid pTy1 into Ty1 loci, budding yeast retrotr...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814769/ https://www.ncbi.nlm.nih.gov/pubmed/31653950 http://dx.doi.org/10.1038/s41598-019-51891-6 |