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DNA methylation of antisense noncoding RNA in the INK locus (ANRIL) is associated with coronary artery disease in a Chinese population

To explore the association between methylation of antisense non-coding RNA in the INK4 locus (ANRIL) and coronary artery disease (CAD) development. Methylation levels of ANRIL in 100 subjects with CAD and 100 controls were quantitatively analyzed using Sequenom MassARRAY. Kyoto Encyclopedia of Genes...

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Autores principales: Zhao, Chen-Hui, Cao, Hai-Tao, Zhang, Jing, Jia, Qiao-Wei, An, Feng-Hui, Chen, Zhao-Hong, Li, Li-Hua, Wang, Lian-Sheng, Ma, Wen-Zhu, Yang, Zhi-Jian, Jia, En-Zhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814794/
https://www.ncbi.nlm.nih.gov/pubmed/31653960
http://dx.doi.org/10.1038/s41598-019-51921-3
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author Zhao, Chen-Hui
Cao, Hai-Tao
Zhang, Jing
Jia, Qiao-Wei
An, Feng-Hui
Chen, Zhao-Hong
Li, Li-Hua
Wang, Lian-Sheng
Ma, Wen-Zhu
Yang, Zhi-Jian
Jia, En-Zhi
author_facet Zhao, Chen-Hui
Cao, Hai-Tao
Zhang, Jing
Jia, Qiao-Wei
An, Feng-Hui
Chen, Zhao-Hong
Li, Li-Hua
Wang, Lian-Sheng
Ma, Wen-Zhu
Yang, Zhi-Jian
Jia, En-Zhi
author_sort Zhao, Chen-Hui
collection PubMed
description To explore the association between methylation of antisense non-coding RNA in the INK4 locus (ANRIL) and coronary artery disease (CAD) development. Methylation levels of ANRIL in 100 subjects with CAD and 100 controls were quantitatively analyzed using Sequenom MassARRAY. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was used to identify novel pathways. Our analyses indicated that 7 to 8 CpG sites within the 2(nd) CpG island located upstream of ANRIL, also known as cyclin-dependent kinase inhibitor 2B – antisense 1 (CDKN2B-AS1), are hyper-methylated in CAD subjects compared to controls (p = 0.034). The 40(th) CpG site within the 2(nd) CpG island located upstream of CDKN2B-AS1 was methylated to a lesser extent in CAD subjects compared to controls (p = 0.045). Both Pearson and Spearman analyses indicated that methylation levels were significantly associated with total cholesterol (r = 0.204, p = 0.004), fasting high-density lipoprotein cholesterol (r = 0.165, p = 0.020), and fasting low-density lipoprotein cholesterol (r = 0.265, p = 0.000). KEGG pathway analysis revealed a significant enrichment of genes associated with the tumor necrosis factor (TNF) signaling pathway. Among them, CCAAT/enhancer binding protein (C/EBPβ) was identified as a key transcription factor that promotes expression of CDKN2B-AS1 through promotor interaction. DNA methylation of the ANRIL promoter was significantly associated with CAD development in our study. Our analyses suggest that C/EBPβ is a key transcription factor that promotes CDKN2B-AS1 expression by directly interacting with the gene promotor mediated by TNF signaling.
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spelling pubmed-68147942019-10-30 DNA methylation of antisense noncoding RNA in the INK locus (ANRIL) is associated with coronary artery disease in a Chinese population Zhao, Chen-Hui Cao, Hai-Tao Zhang, Jing Jia, Qiao-Wei An, Feng-Hui Chen, Zhao-Hong Li, Li-Hua Wang, Lian-Sheng Ma, Wen-Zhu Yang, Zhi-Jian Jia, En-Zhi Sci Rep Article To explore the association between methylation of antisense non-coding RNA in the INK4 locus (ANRIL) and coronary artery disease (CAD) development. Methylation levels of ANRIL in 100 subjects with CAD and 100 controls were quantitatively analyzed using Sequenom MassARRAY. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was used to identify novel pathways. Our analyses indicated that 7 to 8 CpG sites within the 2(nd) CpG island located upstream of ANRIL, also known as cyclin-dependent kinase inhibitor 2B – antisense 1 (CDKN2B-AS1), are hyper-methylated in CAD subjects compared to controls (p = 0.034). The 40(th) CpG site within the 2(nd) CpG island located upstream of CDKN2B-AS1 was methylated to a lesser extent in CAD subjects compared to controls (p = 0.045). Both Pearson and Spearman analyses indicated that methylation levels were significantly associated with total cholesterol (r = 0.204, p = 0.004), fasting high-density lipoprotein cholesterol (r = 0.165, p = 0.020), and fasting low-density lipoprotein cholesterol (r = 0.265, p = 0.000). KEGG pathway analysis revealed a significant enrichment of genes associated with the tumor necrosis factor (TNF) signaling pathway. Among them, CCAAT/enhancer binding protein (C/EBPβ) was identified as a key transcription factor that promotes expression of CDKN2B-AS1 through promotor interaction. DNA methylation of the ANRIL promoter was significantly associated with CAD development in our study. Our analyses suggest that C/EBPβ is a key transcription factor that promotes CDKN2B-AS1 expression by directly interacting with the gene promotor mediated by TNF signaling. Nature Publishing Group UK 2019-10-25 /pmc/articles/PMC6814794/ /pubmed/31653960 http://dx.doi.org/10.1038/s41598-019-51921-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhao, Chen-Hui
Cao, Hai-Tao
Zhang, Jing
Jia, Qiao-Wei
An, Feng-Hui
Chen, Zhao-Hong
Li, Li-Hua
Wang, Lian-Sheng
Ma, Wen-Zhu
Yang, Zhi-Jian
Jia, En-Zhi
DNA methylation of antisense noncoding RNA in the INK locus (ANRIL) is associated with coronary artery disease in a Chinese population
title DNA methylation of antisense noncoding RNA in the INK locus (ANRIL) is associated with coronary artery disease in a Chinese population
title_full DNA methylation of antisense noncoding RNA in the INK locus (ANRIL) is associated with coronary artery disease in a Chinese population
title_fullStr DNA methylation of antisense noncoding RNA in the INK locus (ANRIL) is associated with coronary artery disease in a Chinese population
title_full_unstemmed DNA methylation of antisense noncoding RNA in the INK locus (ANRIL) is associated with coronary artery disease in a Chinese population
title_short DNA methylation of antisense noncoding RNA in the INK locus (ANRIL) is associated with coronary artery disease in a Chinese population
title_sort dna methylation of antisense noncoding rna in the ink locus (anril) is associated with coronary artery disease in a chinese population
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814794/
https://www.ncbi.nlm.nih.gov/pubmed/31653960
http://dx.doi.org/10.1038/s41598-019-51921-3
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