Live cell imaging of macrophage/bacterium interaction demonstrates cell lysis induced by Corynebacterium diphtheriae and Corynebacterium ulcerans

OBJECTIVES: In frame of a study to characterize the interaction of human macrophage-like cells with pathogenic corynebacteria, Corynebacterium diphtheriae and Corynebacterium ulcerans, live cell imaging experiments were carried out and time lapse fluorescence microscopy videos were generated, which...

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Detalles Bibliográficos
Autores principales: Weerasekera, Dulanthi, Hahn, Jonas, Herrmann, Martin, Burkovski, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Data Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6815039/
https://www.ncbi.nlm.nih.gov/pubmed/31653227
http://dx.doi.org/10.1186/s13104-019-4733-y
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author Weerasekera, Dulanthi
Hahn, Jonas
Herrmann, Martin
Burkovski, Andreas
author_facet Weerasekera, Dulanthi
Hahn, Jonas
Herrmann, Martin
Burkovski, Andreas
author_sort Weerasekera, Dulanthi
collection PubMed
description OBJECTIVES: In frame of a study to characterize the interaction of human macrophage-like cells with pathogenic corynebacteria, Corynebacterium diphtheriae and Corynebacterium ulcerans, live cell imaging experiments were carried out and time lapse fluorescence microscopy videos were generated, which are presented here. DATA DESCRIPTION: The time lapse fluorescence microscopy data revealed new insights in the interaction of corynebacteria with human macrophage-like THP-1 cells. In contrast to uninfected cells and infections with non-pathogenic C. glutamicum used as a control, pathogenic C. diphtheriae and C. ulcerans showed highly detrimental effects towards human cells and induction of cell death of macrophages.
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spelling pubmed-68150392019-10-31 Live cell imaging of macrophage/bacterium interaction demonstrates cell lysis induced by Corynebacterium diphtheriae and Corynebacterium ulcerans Weerasekera, Dulanthi Hahn, Jonas Herrmann, Martin Burkovski, Andreas BMC Res Notes Data Note OBJECTIVES: In frame of a study to characterize the interaction of human macrophage-like cells with pathogenic corynebacteria, Corynebacterium diphtheriae and Corynebacterium ulcerans, live cell imaging experiments were carried out and time lapse fluorescence microscopy videos were generated, which are presented here. DATA DESCRIPTION: The time lapse fluorescence microscopy data revealed new insights in the interaction of corynebacteria with human macrophage-like THP-1 cells. In contrast to uninfected cells and infections with non-pathogenic C. glutamicum used as a control, pathogenic C. diphtheriae and C. ulcerans showed highly detrimental effects towards human cells and induction of cell death of macrophages. BioMed Central 2019-10-25 /pmc/articles/PMC6815039/ /pubmed/31653227 http://dx.doi.org/10.1186/s13104-019-4733-y Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Data Note
Weerasekera, Dulanthi
Hahn, Jonas
Herrmann, Martin
Burkovski, Andreas
Live cell imaging of macrophage/bacterium interaction demonstrates cell lysis induced by Corynebacterium diphtheriae and Corynebacterium ulcerans
title Live cell imaging of macrophage/bacterium interaction demonstrates cell lysis induced by Corynebacterium diphtheriae and Corynebacterium ulcerans
title_full Live cell imaging of macrophage/bacterium interaction demonstrates cell lysis induced by Corynebacterium diphtheriae and Corynebacterium ulcerans
title_fullStr Live cell imaging of macrophage/bacterium interaction demonstrates cell lysis induced by Corynebacterium diphtheriae and Corynebacterium ulcerans
title_full_unstemmed Live cell imaging of macrophage/bacterium interaction demonstrates cell lysis induced by Corynebacterium diphtheriae and Corynebacterium ulcerans
title_short Live cell imaging of macrophage/bacterium interaction demonstrates cell lysis induced by Corynebacterium diphtheriae and Corynebacterium ulcerans
title_sort live cell imaging of macrophage/bacterium interaction demonstrates cell lysis induced by corynebacterium diphtheriae and corynebacterium ulcerans
topic Data Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6815039/
https://www.ncbi.nlm.nih.gov/pubmed/31653227
http://dx.doi.org/10.1186/s13104-019-4733-y
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