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A monocentric analysis of the efficacy of extracellular cryoprotectants in unfrozen solutions for cleavage stage embryos

BACKGROUND: In the absence of international guidelines indicating the usage of vitrification rather than slow-freezing, the study aim was to analyze a large cohort of slow-frozen/thawed embryos to produce a rationale supporting the standardization of IVF cryopreservation policy. METHODS: This retros...

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Autores principales: Capodanno, Francesco, Daolio, Jessica, De Feo, Gaetano, Falbo, Angela, Morini, Daria, Nicoli, Alessia, Braglia, Luca, Villani, MariaTeresa, La Sala, Giovanni B., Parmegiani, Lodovico, Aguzzoli, Lorenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6815413/
https://www.ncbi.nlm.nih.gov/pubmed/31656205
http://dx.doi.org/10.1186/s12958-019-0519-2
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author Capodanno, Francesco
Daolio, Jessica
De Feo, Gaetano
Falbo, Angela
Morini, Daria
Nicoli, Alessia
Braglia, Luca
Villani, MariaTeresa
La Sala, Giovanni B.
Parmegiani, Lodovico
Aguzzoli, Lorenzo
author_facet Capodanno, Francesco
Daolio, Jessica
De Feo, Gaetano
Falbo, Angela
Morini, Daria
Nicoli, Alessia
Braglia, Luca
Villani, MariaTeresa
La Sala, Giovanni B.
Parmegiani, Lodovico
Aguzzoli, Lorenzo
author_sort Capodanno, Francesco
collection PubMed
description BACKGROUND: In the absence of international guidelines indicating the usage of vitrification rather than slow-freezing, the study aim was to analyze a large cohort of slow-frozen/thawed embryos to produce a rationale supporting the standardization of IVF cryopreservation policy. METHODS: This retrospective analysis included 4779 cleavage stage embryos cryopreserved by slow-freezing/thawing from September 2009 to April 2017 at a single Center. Biological and clinical outcomes of three different commercial kits adopted sequentially, i.e. Vitrolife Cleave Kit® from Vitrolife (kit 1) vs. K-SICS-5000 Kit® and K-SITS-5000 Kit® from Cook Medical (kit 2) and Freeze/Thaw 1™ Kit® from Vitrolife (kit 3) were collected and compared in the light of cryoprotectants composition. RESULTS: Kit 3 compared to kit 1 and kit 2 showed significantly (P < 0.001) higher embryo survival (79.9% vs. 75.6 and 68.1%, respectively) and frozen embryo replacement (91.5% vs. 86.5 and 83.3%, respectively) rates, and significantly (P < 0.001) lower blastomere degeneration rate (41.5% vs. 43.6 and 52.4%, respectively). No significant difference for clinical outcomes was observed among kits. Only a slight positive trend was observed for kit 3 vs. kit 1 and kit 2 on delivery rate per thawing cycle (7.12% vs. 4.19 and 4.51%, respectively; P < 0.058) and live birth rate (3.07% vs. 2.59 and 1.93%, respectively, P < 0.069). Thawing solutions of kit 3 were similar to those of any warming protocol. CONCLUSIONS: A defined concentration of extracellular cryoprotectants in thawing/warming solutions had a beneficial effect on the embryo cryosurvival rate. Results could provide the rationale for the adoption of a single standardized warming protocol.
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spelling pubmed-68154132019-10-31 A monocentric analysis of the efficacy of extracellular cryoprotectants in unfrozen solutions for cleavage stage embryos Capodanno, Francesco Daolio, Jessica De Feo, Gaetano Falbo, Angela Morini, Daria Nicoli, Alessia Braglia, Luca Villani, MariaTeresa La Sala, Giovanni B. Parmegiani, Lodovico Aguzzoli, Lorenzo Reprod Biol Endocrinol Research BACKGROUND: In the absence of international guidelines indicating the usage of vitrification rather than slow-freezing, the study aim was to analyze a large cohort of slow-frozen/thawed embryos to produce a rationale supporting the standardization of IVF cryopreservation policy. METHODS: This retrospective analysis included 4779 cleavage stage embryos cryopreserved by slow-freezing/thawing from September 2009 to April 2017 at a single Center. Biological and clinical outcomes of three different commercial kits adopted sequentially, i.e. Vitrolife Cleave Kit® from Vitrolife (kit 1) vs. K-SICS-5000 Kit® and K-SITS-5000 Kit® from Cook Medical (kit 2) and Freeze/Thaw 1™ Kit® from Vitrolife (kit 3) were collected and compared in the light of cryoprotectants composition. RESULTS: Kit 3 compared to kit 1 and kit 2 showed significantly (P < 0.001) higher embryo survival (79.9% vs. 75.6 and 68.1%, respectively) and frozen embryo replacement (91.5% vs. 86.5 and 83.3%, respectively) rates, and significantly (P < 0.001) lower blastomere degeneration rate (41.5% vs. 43.6 and 52.4%, respectively). No significant difference for clinical outcomes was observed among kits. Only a slight positive trend was observed for kit 3 vs. kit 1 and kit 2 on delivery rate per thawing cycle (7.12% vs. 4.19 and 4.51%, respectively; P < 0.058) and live birth rate (3.07% vs. 2.59 and 1.93%, respectively, P < 0.069). Thawing solutions of kit 3 were similar to those of any warming protocol. CONCLUSIONS: A defined concentration of extracellular cryoprotectants in thawing/warming solutions had a beneficial effect on the embryo cryosurvival rate. Results could provide the rationale for the adoption of a single standardized warming protocol. BioMed Central 2019-10-27 /pmc/articles/PMC6815413/ /pubmed/31656205 http://dx.doi.org/10.1186/s12958-019-0519-2 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Capodanno, Francesco
Daolio, Jessica
De Feo, Gaetano
Falbo, Angela
Morini, Daria
Nicoli, Alessia
Braglia, Luca
Villani, MariaTeresa
La Sala, Giovanni B.
Parmegiani, Lodovico
Aguzzoli, Lorenzo
A monocentric analysis of the efficacy of extracellular cryoprotectants in unfrozen solutions for cleavage stage embryos
title A monocentric analysis of the efficacy of extracellular cryoprotectants in unfrozen solutions for cleavage stage embryos
title_full A monocentric analysis of the efficacy of extracellular cryoprotectants in unfrozen solutions for cleavage stage embryos
title_fullStr A monocentric analysis of the efficacy of extracellular cryoprotectants in unfrozen solutions for cleavage stage embryos
title_full_unstemmed A monocentric analysis of the efficacy of extracellular cryoprotectants in unfrozen solutions for cleavage stage embryos
title_short A monocentric analysis of the efficacy of extracellular cryoprotectants in unfrozen solutions for cleavage stage embryos
title_sort monocentric analysis of the efficacy of extracellular cryoprotectants in unfrozen solutions for cleavage stage embryos
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6815413/
https://www.ncbi.nlm.nih.gov/pubmed/31656205
http://dx.doi.org/10.1186/s12958-019-0519-2
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