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The cytotoxicity of malathion and essential oil of Nepeta crispa (lamiales: lamiaceae) against vertebrate and invertebrate cell lines

INTRODUCTION: Pesticides are used as essential tools to control vector-borne diseases and agricultural pests and maintain quality and quantity crop production. Scientists attempt to use derived plant natural products due to environmental safety and low mammalian toxicity. Therefore, the cytotoxicity...

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Autores principales: Zahirnia, Amirhossein, Boroomand, Mitra, Nasirian, Hassan, Soleimani-Asl, Sara, Salehzadeh, Aref, Dastan, Dara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The African Field Epidemiology Network 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6815490/
https://www.ncbi.nlm.nih.gov/pubmed/31692877
http://dx.doi.org/10.11604/pamj.2019.33.285.18776
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author Zahirnia, Amirhossein
Boroomand, Mitra
Nasirian, Hassan
Soleimani-Asl, Sara
Salehzadeh, Aref
Dastan, Dara
author_facet Zahirnia, Amirhossein
Boroomand, Mitra
Nasirian, Hassan
Soleimani-Asl, Sara
Salehzadeh, Aref
Dastan, Dara
author_sort Zahirnia, Amirhossein
collection PubMed
description INTRODUCTION: Pesticides are used as essential tools to control vector-borne diseases and agricultural pests and maintain quality and quantity crop production. Scientists attempt to use derived plant natural products due to environmental safety and low mammalian toxicity. Therefore, the cytotoxicity of malathion and Nepeta crispa essential oil against vertebrate L929 and invertebrate Sf9 cell lines were investigated. METHODS: About 2×10(3) cells were placed into the wells of a 96-well plate experiments. Then appropriate concentrations of malathion and N. crispa essential oil added to the wells. The cells were allowed to grow for 3-5 days and estimated the cell numbers. Control cell wells contained only cells with DMSO. All treatments and controls repeated at least four replicates. RESULTS: About 2×10(3) cells were placed into the wells of a 96-well plate experiments. Then appropriate concentrations of malathion and N. crispa essential oil added to the wells. The cells were allowed to grow for 3-5 days and estimated the cell numbers. Control cell wells contained only cells with DMSO. All treatments and controls repeated at least four replicates. CONCLUSION: Plant essential oil not only had no negative effects but also had boosting effects on the L929 cell viability. Nepeta crispa essential oil had negative effects on the Sf9 cell viability with the differences that derived plant natural products containing environmentally friendly and readily biodegradable derivatives, hydrolyzing rapidly in nature and nearly having no destructive effects on mammals and environment.
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spelling pubmed-68154902019-11-05 The cytotoxicity of malathion and essential oil of Nepeta crispa (lamiales: lamiaceae) against vertebrate and invertebrate cell lines Zahirnia, Amirhossein Boroomand, Mitra Nasirian, Hassan Soleimani-Asl, Sara Salehzadeh, Aref Dastan, Dara Pan Afr Med J Research INTRODUCTION: Pesticides are used as essential tools to control vector-borne diseases and agricultural pests and maintain quality and quantity crop production. Scientists attempt to use derived plant natural products due to environmental safety and low mammalian toxicity. Therefore, the cytotoxicity of malathion and Nepeta crispa essential oil against vertebrate L929 and invertebrate Sf9 cell lines were investigated. METHODS: About 2×10(3) cells were placed into the wells of a 96-well plate experiments. Then appropriate concentrations of malathion and N. crispa essential oil added to the wells. The cells were allowed to grow for 3-5 days and estimated the cell numbers. Control cell wells contained only cells with DMSO. All treatments and controls repeated at least four replicates. RESULTS: About 2×10(3) cells were placed into the wells of a 96-well plate experiments. Then appropriate concentrations of malathion and N. crispa essential oil added to the wells. The cells were allowed to grow for 3-5 days and estimated the cell numbers. Control cell wells contained only cells with DMSO. All treatments and controls repeated at least four replicates. CONCLUSION: Plant essential oil not only had no negative effects but also had boosting effects on the L929 cell viability. Nepeta crispa essential oil had negative effects on the Sf9 cell viability with the differences that derived plant natural products containing environmentally friendly and readily biodegradable derivatives, hydrolyzing rapidly in nature and nearly having no destructive effects on mammals and environment. The African Field Epidemiology Network 2019-08-06 /pmc/articles/PMC6815490/ /pubmed/31692877 http://dx.doi.org/10.11604/pamj.2019.33.285.18776 Text en © Amirhossein Zahirnia et al. http://creativecommons.org/licenses/by/2.0/ The Pan African Medical Journal - ISSN 1937-8688. This is an Open Access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Zahirnia, Amirhossein
Boroomand, Mitra
Nasirian, Hassan
Soleimani-Asl, Sara
Salehzadeh, Aref
Dastan, Dara
The cytotoxicity of malathion and essential oil of Nepeta crispa (lamiales: lamiaceae) against vertebrate and invertebrate cell lines
title The cytotoxicity of malathion and essential oil of Nepeta crispa (lamiales: lamiaceae) against vertebrate and invertebrate cell lines
title_full The cytotoxicity of malathion and essential oil of Nepeta crispa (lamiales: lamiaceae) against vertebrate and invertebrate cell lines
title_fullStr The cytotoxicity of malathion and essential oil of Nepeta crispa (lamiales: lamiaceae) against vertebrate and invertebrate cell lines
title_full_unstemmed The cytotoxicity of malathion and essential oil of Nepeta crispa (lamiales: lamiaceae) against vertebrate and invertebrate cell lines
title_short The cytotoxicity of malathion and essential oil of Nepeta crispa (lamiales: lamiaceae) against vertebrate and invertebrate cell lines
title_sort cytotoxicity of malathion and essential oil of nepeta crispa (lamiales: lamiaceae) against vertebrate and invertebrate cell lines
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6815490/
https://www.ncbi.nlm.nih.gov/pubmed/31692877
http://dx.doi.org/10.11604/pamj.2019.33.285.18776
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