Cargando…

Increased Gene Expression of RUNX2 and SOX9 in Mesenchymal Circulating Progenitors Is Associated with Autophagy during Physical Activity

Lack of physical exercise is considered an important risk factor for chronic diseases. On the contrary, physical exercise reduces the morbidity rates of obesity, diabetes, bone disease, and hypertension. In order to gain novel molecular and cellular clues, we analyzed the effects of physical exercis...

Descripción completa

Detalles Bibliográficos
Autores principales: Dalle Carbonare, L., Mottes, M., Cheri, S., Deiana, M., Zamboni, F., Gabbiani, D., Schena, F., Salvagno, G. L., Lippi, G., Valenti, M. T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6815530/
https://www.ncbi.nlm.nih.gov/pubmed/31737174
http://dx.doi.org/10.1155/2019/8426259
_version_ 1783463202333392896
author Dalle Carbonare, L.
Mottes, M.
Cheri, S.
Deiana, M.
Zamboni, F.
Gabbiani, D.
Schena, F.
Salvagno, G. L.
Lippi, G.
Valenti, M. T.
author_facet Dalle Carbonare, L.
Mottes, M.
Cheri, S.
Deiana, M.
Zamboni, F.
Gabbiani, D.
Schena, F.
Salvagno, G. L.
Lippi, G.
Valenti, M. T.
author_sort Dalle Carbonare, L.
collection PubMed
description Lack of physical exercise is considered an important risk factor for chronic diseases. On the contrary, physical exercise reduces the morbidity rates of obesity, diabetes, bone disease, and hypertension. In order to gain novel molecular and cellular clues, we analyzed the effects of physical exercise on differentiation of mesenchymal circulating progenitor cells (M-CPCs) obtained from runners. We also investigated autophagy and telomerase-related gene expression to evaluate the involvement of specific cellular functions in the differentiation process. We performed cellular and molecular analyses in M-CPCs, obtained by a depletion method, of 22 subjects before (PRE RUN) and after (POST RUN) a half marathon performance. In order to prove our findings, we performed also in vitro analyses by testing the effects of runners' sera on a human bone marrow-derived mesenchymal stem (hBM-MSC) cell line. PCR array analyses of PRE RUN versus POST RUN M-CPC total RNAs put in evidence several genes which appeared to be modulated by physical activity. Our results showed that physical exercise promotes differentiation. Osteogenesis-related genes as RUNX2, MSX1, and SPP1 appeared to be upregulated after the run; data showed also increased levels of BMP2 and BMP6 expressions. SOX9, COL2A1, and COMP gene enhanced expression suggested the induction of chondrocytic differentiation as well. The expression of telomerase-associated genes and of two autophagy-related genes, ATG3 and ULK1, was also affected and correlated positively with MSC differentiation. These data highlight an attractive cellular scenario, outlining the role of autophagic response to physical exercise and suggesting new insights into the benefits of physical exercise in counteracting chronic degenerative conditions.
format Online
Article
Text
id pubmed-6815530
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-68155302019-11-17 Increased Gene Expression of RUNX2 and SOX9 in Mesenchymal Circulating Progenitors Is Associated with Autophagy during Physical Activity Dalle Carbonare, L. Mottes, M. Cheri, S. Deiana, M. Zamboni, F. Gabbiani, D. Schena, F. Salvagno, G. L. Lippi, G. Valenti, M. T. Oxid Med Cell Longev Research Article Lack of physical exercise is considered an important risk factor for chronic diseases. On the contrary, physical exercise reduces the morbidity rates of obesity, diabetes, bone disease, and hypertension. In order to gain novel molecular and cellular clues, we analyzed the effects of physical exercise on differentiation of mesenchymal circulating progenitor cells (M-CPCs) obtained from runners. We also investigated autophagy and telomerase-related gene expression to evaluate the involvement of specific cellular functions in the differentiation process. We performed cellular and molecular analyses in M-CPCs, obtained by a depletion method, of 22 subjects before (PRE RUN) and after (POST RUN) a half marathon performance. In order to prove our findings, we performed also in vitro analyses by testing the effects of runners' sera on a human bone marrow-derived mesenchymal stem (hBM-MSC) cell line. PCR array analyses of PRE RUN versus POST RUN M-CPC total RNAs put in evidence several genes which appeared to be modulated by physical activity. Our results showed that physical exercise promotes differentiation. Osteogenesis-related genes as RUNX2, MSX1, and SPP1 appeared to be upregulated after the run; data showed also increased levels of BMP2 and BMP6 expressions. SOX9, COL2A1, and COMP gene enhanced expression suggested the induction of chondrocytic differentiation as well. The expression of telomerase-associated genes and of two autophagy-related genes, ATG3 and ULK1, was also affected and correlated positively with MSC differentiation. These data highlight an attractive cellular scenario, outlining the role of autophagic response to physical exercise and suggesting new insights into the benefits of physical exercise in counteracting chronic degenerative conditions. Hindawi 2019-10-15 /pmc/articles/PMC6815530/ /pubmed/31737174 http://dx.doi.org/10.1155/2019/8426259 Text en Copyright © 2019 L. Dalle Carbonare et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dalle Carbonare, L.
Mottes, M.
Cheri, S.
Deiana, M.
Zamboni, F.
Gabbiani, D.
Schena, F.
Salvagno, G. L.
Lippi, G.
Valenti, M. T.
Increased Gene Expression of RUNX2 and SOX9 in Mesenchymal Circulating Progenitors Is Associated with Autophagy during Physical Activity
title Increased Gene Expression of RUNX2 and SOX9 in Mesenchymal Circulating Progenitors Is Associated with Autophagy during Physical Activity
title_full Increased Gene Expression of RUNX2 and SOX9 in Mesenchymal Circulating Progenitors Is Associated with Autophagy during Physical Activity
title_fullStr Increased Gene Expression of RUNX2 and SOX9 in Mesenchymal Circulating Progenitors Is Associated with Autophagy during Physical Activity
title_full_unstemmed Increased Gene Expression of RUNX2 and SOX9 in Mesenchymal Circulating Progenitors Is Associated with Autophagy during Physical Activity
title_short Increased Gene Expression of RUNX2 and SOX9 in Mesenchymal Circulating Progenitors Is Associated with Autophagy during Physical Activity
title_sort increased gene expression of runx2 and sox9 in mesenchymal circulating progenitors is associated with autophagy during physical activity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6815530/
https://www.ncbi.nlm.nih.gov/pubmed/31737174
http://dx.doi.org/10.1155/2019/8426259
work_keys_str_mv AT dallecarbonarel increasedgeneexpressionofrunx2andsox9inmesenchymalcirculatingprogenitorsisassociatedwithautophagyduringphysicalactivity
AT mottesm increasedgeneexpressionofrunx2andsox9inmesenchymalcirculatingprogenitorsisassociatedwithautophagyduringphysicalactivity
AT cheris increasedgeneexpressionofrunx2andsox9inmesenchymalcirculatingprogenitorsisassociatedwithautophagyduringphysicalactivity
AT deianam increasedgeneexpressionofrunx2andsox9inmesenchymalcirculatingprogenitorsisassociatedwithautophagyduringphysicalactivity
AT zambonif increasedgeneexpressionofrunx2andsox9inmesenchymalcirculatingprogenitorsisassociatedwithautophagyduringphysicalactivity
AT gabbianid increasedgeneexpressionofrunx2andsox9inmesenchymalcirculatingprogenitorsisassociatedwithautophagyduringphysicalactivity
AT schenaf increasedgeneexpressionofrunx2andsox9inmesenchymalcirculatingprogenitorsisassociatedwithautophagyduringphysicalactivity
AT salvagnogl increasedgeneexpressionofrunx2andsox9inmesenchymalcirculatingprogenitorsisassociatedwithautophagyduringphysicalactivity
AT lippig increasedgeneexpressionofrunx2andsox9inmesenchymalcirculatingprogenitorsisassociatedwithautophagyduringphysicalactivity
AT valentimt increasedgeneexpressionofrunx2andsox9inmesenchymalcirculatingprogenitorsisassociatedwithautophagyduringphysicalactivity