Cargando…

Three-Dimensional Shapes and Cell Deformability of Rat Red Blood Cells during and after Asphyxial Cardiac Arrest

Changes in microcirculation are believed to perform an important role after cardiac arrest. In particular, rheological changes in red blood cells (RBCs) have been observed during and after ischemic-reperfusion injury. Employing three-dimensional laser interferometric microscopy, we investigated thre...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Hui Jai, Lee, SangYun, Park, HyunJoo, Park, YongKeun, Shin, Jonghwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6815595/
https://www.ncbi.nlm.nih.gov/pubmed/31737367
http://dx.doi.org/10.1155/2019/6027236
_version_ 1783463219640139776
author Lee, Hui Jai
Lee, SangYun
Park, HyunJoo
Park, YongKeun
Shin, Jonghwan
author_facet Lee, Hui Jai
Lee, SangYun
Park, HyunJoo
Park, YongKeun
Shin, Jonghwan
author_sort Lee, Hui Jai
collection PubMed
description Changes in microcirculation are believed to perform an important role after cardiac arrest. In particular, rheological changes in red blood cells (RBCs) have been observed during and after ischemic-reperfusion injury. Employing three-dimensional laser interferometric microscopy, we investigated three-dimensional shapes and deformability of RBCs during and after asphyxial cardiac arrest in rats at the individual cell level. Rat cardiac arrest was induced by asphyxia. Five rats were maintained for 7 min of no-flow time, and then, cardiopulmonary resuscitation (CPR) was started. Blood samples were obtained before cardiac arrest, during CPR, and 60 min after return of spontaneous circulation (ROSC). Quantitative phase imaging (QPI) techniques based on laser interferometry were used to measure the three-dimensional refractive index (RI) tomograms of the RBC, from which structural and biochemical properties were retrieved. Dynamic membrane fluctuations in the cell membrane were also quantitatively and sensitively measured in order to investigate cell deformability. Mean corpuscular hemoglobin, mean cell volume, mean corpuscular hemoglobin concentration, and red blood cell distribution width remained unchanged during CPR and after ROSC compared with those before cardiac arrest. QPI results revealed that RBC membrane fluctuations, sphericity, and surface area did not change significantly during CPR or after ROSC compared with initial values. In conclusion, no three-dimensional shapes and cell deformability changes in RBCs were detected.
format Online
Article
Text
id pubmed-6815595
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-68155952019-11-17 Three-Dimensional Shapes and Cell Deformability of Rat Red Blood Cells during and after Asphyxial Cardiac Arrest Lee, Hui Jai Lee, SangYun Park, HyunJoo Park, YongKeun Shin, Jonghwan Emerg Med Int Research Article Changes in microcirculation are believed to perform an important role after cardiac arrest. In particular, rheological changes in red blood cells (RBCs) have been observed during and after ischemic-reperfusion injury. Employing three-dimensional laser interferometric microscopy, we investigated three-dimensional shapes and deformability of RBCs during and after asphyxial cardiac arrest in rats at the individual cell level. Rat cardiac arrest was induced by asphyxia. Five rats were maintained for 7 min of no-flow time, and then, cardiopulmonary resuscitation (CPR) was started. Blood samples were obtained before cardiac arrest, during CPR, and 60 min after return of spontaneous circulation (ROSC). Quantitative phase imaging (QPI) techniques based on laser interferometry were used to measure the three-dimensional refractive index (RI) tomograms of the RBC, from which structural and biochemical properties were retrieved. Dynamic membrane fluctuations in the cell membrane were also quantitatively and sensitively measured in order to investigate cell deformability. Mean corpuscular hemoglobin, mean cell volume, mean corpuscular hemoglobin concentration, and red blood cell distribution width remained unchanged during CPR and after ROSC compared with those before cardiac arrest. QPI results revealed that RBC membrane fluctuations, sphericity, and surface area did not change significantly during CPR or after ROSC compared with initial values. In conclusion, no three-dimensional shapes and cell deformability changes in RBCs were detected. Hindawi 2019-10-15 /pmc/articles/PMC6815595/ /pubmed/31737367 http://dx.doi.org/10.1155/2019/6027236 Text en Copyright © 2019 Hui Jai Lee et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lee, Hui Jai
Lee, SangYun
Park, HyunJoo
Park, YongKeun
Shin, Jonghwan
Three-Dimensional Shapes and Cell Deformability of Rat Red Blood Cells during and after Asphyxial Cardiac Arrest
title Three-Dimensional Shapes and Cell Deformability of Rat Red Blood Cells during and after Asphyxial Cardiac Arrest
title_full Three-Dimensional Shapes and Cell Deformability of Rat Red Blood Cells during and after Asphyxial Cardiac Arrest
title_fullStr Three-Dimensional Shapes and Cell Deformability of Rat Red Blood Cells during and after Asphyxial Cardiac Arrest
title_full_unstemmed Three-Dimensional Shapes and Cell Deformability of Rat Red Blood Cells during and after Asphyxial Cardiac Arrest
title_short Three-Dimensional Shapes and Cell Deformability of Rat Red Blood Cells during and after Asphyxial Cardiac Arrest
title_sort three-dimensional shapes and cell deformability of rat red blood cells during and after asphyxial cardiac arrest
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6815595/
https://www.ncbi.nlm.nih.gov/pubmed/31737367
http://dx.doi.org/10.1155/2019/6027236
work_keys_str_mv AT leehuijai threedimensionalshapesandcelldeformabilityofratredbloodcellsduringandafterasphyxialcardiacarrest
AT leesangyun threedimensionalshapesandcelldeformabilityofratredbloodcellsduringandafterasphyxialcardiacarrest
AT parkhyunjoo threedimensionalshapesandcelldeformabilityofratredbloodcellsduringandafterasphyxialcardiacarrest
AT parkyongkeun threedimensionalshapesandcelldeformabilityofratredbloodcellsduringandafterasphyxialcardiacarrest
AT shinjonghwan threedimensionalshapesandcelldeformabilityofratredbloodcellsduringandafterasphyxialcardiacarrest