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Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus

Involvement of the central nervous system (CNS) is an uncommon feature in systemic lupus erythematosus (SLE), making diagnosis rather difficult and challenging due to the poor specificity of neuropathic symptoms and neurological symptoms. In this work, we used human‐induced pluripotent stem cells (h...

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Autores principales: De Angelis, Maria Teresa, Santamaria, Gianluca, Parrotta, Elvira Immacolata, Scalise, Stefania, Lo Conte, Michela, Gasparini, Sara, Ferlazzo, Edoardo, Aguglia, Umberto, Ciampi, Clara, Sgura, Antonella, Cuda, Giovanni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6815917/
https://www.ncbi.nlm.nih.gov/pubmed/31536674
http://dx.doi.org/10.1111/jcmm.14598
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author De Angelis, Maria Teresa
Santamaria, Gianluca
Parrotta, Elvira Immacolata
Scalise, Stefania
Lo Conte, Michela
Gasparini, Sara
Ferlazzo, Edoardo
Aguglia, Umberto
Ciampi, Clara
Sgura, Antonella
Cuda, Giovanni
author_facet De Angelis, Maria Teresa
Santamaria, Gianluca
Parrotta, Elvira Immacolata
Scalise, Stefania
Lo Conte, Michela
Gasparini, Sara
Ferlazzo, Edoardo
Aguglia, Umberto
Ciampi, Clara
Sgura, Antonella
Cuda, Giovanni
author_sort De Angelis, Maria Teresa
collection PubMed
description Involvement of the central nervous system (CNS) is an uncommon feature in systemic lupus erythematosus (SLE), making diagnosis rather difficult and challenging due to the poor specificity of neuropathic symptoms and neurological symptoms. In this work, we used human‐induced pluripotent stem cells (hiPSCs) derived from CNS‐SLE patient, with the aim to dissect the molecular insights underlying the disease by gene expression analysis and modulation of implicated pathways. CNS‐SLE‐derived hiPSCs allowed us to provide evidence of Erk and Akt pathways involvement and to identify a novel cohort of potential biomarkers, namely CHCHD2, IDO1, S100A10, EPHA4 and LEFTY1, never reported so far. We further extended the study analysing a panel of oxidative stress‐related miRNAs and demonstrated, under normal or stress conditions, a strong dysregulation of several miRNAs in CNS‐SLE‐derived compared to control hiPSCs. In conclusion, we provide evidence that iPSCs reprogrammed from CNS‐SLE patient are a powerful useful tool to investigate the molecular mechanisms underlying the disease and to eventually develop innovative therapeutic approaches.
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spelling pubmed-68159172019-11-01 Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus De Angelis, Maria Teresa Santamaria, Gianluca Parrotta, Elvira Immacolata Scalise, Stefania Lo Conte, Michela Gasparini, Sara Ferlazzo, Edoardo Aguglia, Umberto Ciampi, Clara Sgura, Antonella Cuda, Giovanni J Cell Mol Med Original Articles Involvement of the central nervous system (CNS) is an uncommon feature in systemic lupus erythematosus (SLE), making diagnosis rather difficult and challenging due to the poor specificity of neuropathic symptoms and neurological symptoms. In this work, we used human‐induced pluripotent stem cells (hiPSCs) derived from CNS‐SLE patient, with the aim to dissect the molecular insights underlying the disease by gene expression analysis and modulation of implicated pathways. CNS‐SLE‐derived hiPSCs allowed us to provide evidence of Erk and Akt pathways involvement and to identify a novel cohort of potential biomarkers, namely CHCHD2, IDO1, S100A10, EPHA4 and LEFTY1, never reported so far. We further extended the study analysing a panel of oxidative stress‐related miRNAs and demonstrated, under normal or stress conditions, a strong dysregulation of several miRNAs in CNS‐SLE‐derived compared to control hiPSCs. In conclusion, we provide evidence that iPSCs reprogrammed from CNS‐SLE patient are a powerful useful tool to investigate the molecular mechanisms underlying the disease and to eventually develop innovative therapeutic approaches. John Wiley and Sons Inc. 2019-09-19 2019-11 /pmc/articles/PMC6815917/ /pubmed/31536674 http://dx.doi.org/10.1111/jcmm.14598 Text en © 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
De Angelis, Maria Teresa
Santamaria, Gianluca
Parrotta, Elvira Immacolata
Scalise, Stefania
Lo Conte, Michela
Gasparini, Sara
Ferlazzo, Edoardo
Aguglia, Umberto
Ciampi, Clara
Sgura, Antonella
Cuda, Giovanni
Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus
title Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus
title_full Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus
title_fullStr Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus
title_full_unstemmed Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus
title_short Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus
title_sort establishment and characterization of induced pluripotent stem cells (ipscs) from central nervous system lupus erythematosus
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6815917/
https://www.ncbi.nlm.nih.gov/pubmed/31536674
http://dx.doi.org/10.1111/jcmm.14598
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