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Shigella-mediated oxygen depletion is essential for intestinal mucosa colonization

Pathogenic enterobacteria face various oxygen (O(2)) levels during intestinal colonization from the O(2)-deprived lumen to oxygenated tissues. Using Shigella flexneri as a model, we had previously demonstrated that epithelium invasion is promoted by O(2) in a Type III secretion system (T3SS)-depende...

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Autores principales: Tinevez, Jean-Yves, Arena, Ellen T, Anderson, Mark C, Nigro, Giulia, Injarabian, Louise, André, Antonin C, Ferrari, Mariana, Campbell-Valois, François-Xavier, Devin, Anne, Shorte, Spencer L, Sansonetti, Philippe J, Marteyn, Benoit S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6817363/
https://www.ncbi.nlm.nih.gov/pubmed/31383999
http://dx.doi.org/10.1038/s41564-019-0525-3
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author Tinevez, Jean-Yves
Arena, Ellen T
Anderson, Mark C
Nigro, Giulia
Injarabian, Louise
André, Antonin C
Ferrari, Mariana
Campbell-Valois, François-Xavier
Devin, Anne
Shorte, Spencer L
Sansonetti, Philippe J
Marteyn, Benoit S
author_facet Tinevez, Jean-Yves
Arena, Ellen T
Anderson, Mark C
Nigro, Giulia
Injarabian, Louise
André, Antonin C
Ferrari, Mariana
Campbell-Valois, François-Xavier
Devin, Anne
Shorte, Spencer L
Sansonetti, Philippe J
Marteyn, Benoit S
author_sort Tinevez, Jean-Yves
collection PubMed
description Pathogenic enterobacteria face various oxygen (O(2)) levels during intestinal colonization from the O(2)-deprived lumen to oxygenated tissues. Using Shigella flexneri as a model, we had previously demonstrated that epithelium invasion is promoted by O(2) in a Type III secretion system (T3SS)-dependent manner(1). However, subsequent pathogen adaptation to tissue oxygenation modulation remained unknown. Assessing single-cell distribution, together with tissue oxygenation, we demonstrate here that the colonic mucosa O(2) is actively depleted by Shigella flexneri aerobic respiration, not host neutrophils, during infection, leading to the formation of hypoxic foci of infection. This process is promoted by T3SS inactivation in infected tissues, favoring colonizers over explorers. We identify the molecular mechanisms supporting infectious hypoxia induction, and we demonstrate here how enteropathogens optimize their colonization capacity in relation to their ability to manipulate tissue oxygenation during infection.
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spelling pubmed-68173632020-02-05 Shigella-mediated oxygen depletion is essential for intestinal mucosa colonization Tinevez, Jean-Yves Arena, Ellen T Anderson, Mark C Nigro, Giulia Injarabian, Louise André, Antonin C Ferrari, Mariana Campbell-Valois, François-Xavier Devin, Anne Shorte, Spencer L Sansonetti, Philippe J Marteyn, Benoit S Nat Microbiol Article Pathogenic enterobacteria face various oxygen (O(2)) levels during intestinal colonization from the O(2)-deprived lumen to oxygenated tissues. Using Shigella flexneri as a model, we had previously demonstrated that epithelium invasion is promoted by O(2) in a Type III secretion system (T3SS)-dependent manner(1). However, subsequent pathogen adaptation to tissue oxygenation modulation remained unknown. Assessing single-cell distribution, together with tissue oxygenation, we demonstrate here that the colonic mucosa O(2) is actively depleted by Shigella flexneri aerobic respiration, not host neutrophils, during infection, leading to the formation of hypoxic foci of infection. This process is promoted by T3SS inactivation in infected tissues, favoring colonizers over explorers. We identify the molecular mechanisms supporting infectious hypoxia induction, and we demonstrate here how enteropathogens optimize their colonization capacity in relation to their ability to manipulate tissue oxygenation during infection. 2019-11-01 2019-08-05 /pmc/articles/PMC6817363/ /pubmed/31383999 http://dx.doi.org/10.1038/s41564-019-0525-3 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Tinevez, Jean-Yves
Arena, Ellen T
Anderson, Mark C
Nigro, Giulia
Injarabian, Louise
André, Antonin C
Ferrari, Mariana
Campbell-Valois, François-Xavier
Devin, Anne
Shorte, Spencer L
Sansonetti, Philippe J
Marteyn, Benoit S
Shigella-mediated oxygen depletion is essential for intestinal mucosa colonization
title Shigella-mediated oxygen depletion is essential for intestinal mucosa colonization
title_full Shigella-mediated oxygen depletion is essential for intestinal mucosa colonization
title_fullStr Shigella-mediated oxygen depletion is essential for intestinal mucosa colonization
title_full_unstemmed Shigella-mediated oxygen depletion is essential for intestinal mucosa colonization
title_short Shigella-mediated oxygen depletion is essential for intestinal mucosa colonization
title_sort shigella-mediated oxygen depletion is essential for intestinal mucosa colonization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6817363/
https://www.ncbi.nlm.nih.gov/pubmed/31383999
http://dx.doi.org/10.1038/s41564-019-0525-3
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