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Adrenal Steroids Modulate Fibroblast-Like Synoviocytes Response During B. abortus Infection

Brucella abortus stimulates an inflammatory immune response that stimulates the endocrine system, inducing the secretion of dehydroepiandrosterone (DHEA) and cortisol. In humans, the active disease is generally present as osteoarticular brucellosis. In previous studies we showed that B. abortus infe...

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Autores principales: Gentilini, María Virginia, Giambartolomei, Guillermo Hernán, Delpino, María Victoria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6817619/
https://www.ncbi.nlm.nih.gov/pubmed/31695682
http://dx.doi.org/10.3389/fendo.2019.00722
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author Gentilini, María Virginia
Giambartolomei, Guillermo Hernán
Delpino, María Victoria
author_facet Gentilini, María Virginia
Giambartolomei, Guillermo Hernán
Delpino, María Victoria
author_sort Gentilini, María Virginia
collection PubMed
description Brucella abortus stimulates an inflammatory immune response that stimulates the endocrine system, inducing the secretion of dehydroepiandrosterone (DHEA) and cortisol. In humans, the active disease is generally present as osteoarticular brucellosis. In previous studies we showed that B. abortus infection of synoviocytes creates a proinflammatory microenvironment. We proposed to determine the role of cortisol and DHEA on synoviocytes and infiltrating monocytes during B. abortus infection. Cortisol inhibited IL-6, IL-8, MCP-1, and MMP-2 secretion induced by B. abortus infection in synovial fibroblast. Cortisol-mediated MMP-2 inhibition during B. abortus infection was reversed by IL-6. DHEA inhibited B. abortus-induced RANKL up-regulation in synovial fibroblast through estrogen receptor (ER). B. abortus infection did not modulate glucocorticoid receptor (GR) expression. Cell responses to cortisol also depended on its intracellular bioavailability, according to the activity of the isoenzymes 11β-hydroxysteroid dehydrogenase (HSD) type-1 and 11β-HSD2 (which are involved in cortisone-cortisol interconversion). B. abortus infection did not modify 11β-HSD1 expression and GRα/β ratio in the presence or absence of adrenal steroids. Supernatants from B. abortus-infected monocytes induced 11β-HSD1 in synovial cells. Administration of cortisone was capable of inhibiting the secretion of RANKL by synoviocytes mimicking cortisol's effect. These results go along with previous observations that highlighted the ability of synovial tissue to secrete active steroids, making it an intracrine tissue. This is the first study that contributes to the knowledge of the consequence of adrenal steroids on synoviocytes in the context of a bacterial infection.
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spelling pubmed-68176192019-11-06 Adrenal Steroids Modulate Fibroblast-Like Synoviocytes Response During B. abortus Infection Gentilini, María Virginia Giambartolomei, Guillermo Hernán Delpino, María Victoria Front Endocrinol (Lausanne) Endocrinology Brucella abortus stimulates an inflammatory immune response that stimulates the endocrine system, inducing the secretion of dehydroepiandrosterone (DHEA) and cortisol. In humans, the active disease is generally present as osteoarticular brucellosis. In previous studies we showed that B. abortus infection of synoviocytes creates a proinflammatory microenvironment. We proposed to determine the role of cortisol and DHEA on synoviocytes and infiltrating monocytes during B. abortus infection. Cortisol inhibited IL-6, IL-8, MCP-1, and MMP-2 secretion induced by B. abortus infection in synovial fibroblast. Cortisol-mediated MMP-2 inhibition during B. abortus infection was reversed by IL-6. DHEA inhibited B. abortus-induced RANKL up-regulation in synovial fibroblast through estrogen receptor (ER). B. abortus infection did not modulate glucocorticoid receptor (GR) expression. Cell responses to cortisol also depended on its intracellular bioavailability, according to the activity of the isoenzymes 11β-hydroxysteroid dehydrogenase (HSD) type-1 and 11β-HSD2 (which are involved in cortisone-cortisol interconversion). B. abortus infection did not modify 11β-HSD1 expression and GRα/β ratio in the presence or absence of adrenal steroids. Supernatants from B. abortus-infected monocytes induced 11β-HSD1 in synovial cells. Administration of cortisone was capable of inhibiting the secretion of RANKL by synoviocytes mimicking cortisol's effect. These results go along with previous observations that highlighted the ability of synovial tissue to secrete active steroids, making it an intracrine tissue. This is the first study that contributes to the knowledge of the consequence of adrenal steroids on synoviocytes in the context of a bacterial infection. Frontiers Media S.A. 2019-10-22 /pmc/articles/PMC6817619/ /pubmed/31695682 http://dx.doi.org/10.3389/fendo.2019.00722 Text en Copyright © 2019 Gentilini, Giambartolomei and Delpino. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Gentilini, María Virginia
Giambartolomei, Guillermo Hernán
Delpino, María Victoria
Adrenal Steroids Modulate Fibroblast-Like Synoviocytes Response During B. abortus Infection
title Adrenal Steroids Modulate Fibroblast-Like Synoviocytes Response During B. abortus Infection
title_full Adrenal Steroids Modulate Fibroblast-Like Synoviocytes Response During B. abortus Infection
title_fullStr Adrenal Steroids Modulate Fibroblast-Like Synoviocytes Response During B. abortus Infection
title_full_unstemmed Adrenal Steroids Modulate Fibroblast-Like Synoviocytes Response During B. abortus Infection
title_short Adrenal Steroids Modulate Fibroblast-Like Synoviocytes Response During B. abortus Infection
title_sort adrenal steroids modulate fibroblast-like synoviocytes response during b. abortus infection
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6817619/
https://www.ncbi.nlm.nih.gov/pubmed/31695682
http://dx.doi.org/10.3389/fendo.2019.00722
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