Cargando…
Human Cytomegalovirus Upregulates Expression of HCLS1 Resulting in Increased Cell Motility and Transendothelial Migration during Latency
Human cytomegalovirus establishes a lifelong, latent infection in the human host and can cause significant morbidity and mortality, particularly, in immunocompromised individuals. One established site of HCMV latency and reactivation is in cells of the myeloid lineage. In undifferentiated myeloid ce...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6817630/ https://www.ncbi.nlm.nih.gov/pubmed/31569051 http://dx.doi.org/10.1016/j.isci.2019.09.016 |
_version_ | 1783463463140458496 |
---|---|
author | Aslam, Yusuf Williamson, James Romashova, Veronika Elder, Elizabeth Krishna, Benjamin Wills, Mark Lehner, Paul Sinclair, John Poole, Emma |
author_facet | Aslam, Yusuf Williamson, James Romashova, Veronika Elder, Elizabeth Krishna, Benjamin Wills, Mark Lehner, Paul Sinclair, John Poole, Emma |
author_sort | Aslam, Yusuf |
collection | PubMed |
description | Human cytomegalovirus establishes a lifelong, latent infection in the human host and can cause significant morbidity and mortality, particularly, in immunocompromised individuals. One established site of HCMV latency and reactivation is in cells of the myeloid lineage. In undifferentiated myeloid cells, such as CD14+ monocytes, virus is maintained latently. We have recently reported an analysis of the total proteome of latently infected CD14+ monocytes, which identified an increase in hematopoietic lineage cell-specific protein (HCLS1). Here we show that this latency-associated upregulation of HCLS1 occurs in a US28-dependent manner and stabilizes actin structure in latently infected cells. This results in their increased motility and ability to transit endothelial cell layers. Thus, latency-associated increases in monocyte motility could aid dissemination of the latently infected reservoir, and targeting this increased motility could have an impact on the ability of latently infected monocytes to distribute to tissue sites of reactivation. |
format | Online Article Text |
id | pubmed-6817630 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-68176302019-10-31 Human Cytomegalovirus Upregulates Expression of HCLS1 Resulting in Increased Cell Motility and Transendothelial Migration during Latency Aslam, Yusuf Williamson, James Romashova, Veronika Elder, Elizabeth Krishna, Benjamin Wills, Mark Lehner, Paul Sinclair, John Poole, Emma iScience Article Human cytomegalovirus establishes a lifelong, latent infection in the human host and can cause significant morbidity and mortality, particularly, in immunocompromised individuals. One established site of HCMV latency and reactivation is in cells of the myeloid lineage. In undifferentiated myeloid cells, such as CD14+ monocytes, virus is maintained latently. We have recently reported an analysis of the total proteome of latently infected CD14+ monocytes, which identified an increase in hematopoietic lineage cell-specific protein (HCLS1). Here we show that this latency-associated upregulation of HCLS1 occurs in a US28-dependent manner and stabilizes actin structure in latently infected cells. This results in their increased motility and ability to transit endothelial cell layers. Thus, latency-associated increases in monocyte motility could aid dissemination of the latently infected reservoir, and targeting this increased motility could have an impact on the ability of latently infected monocytes to distribute to tissue sites of reactivation. Elsevier 2019-09-14 /pmc/articles/PMC6817630/ /pubmed/31569051 http://dx.doi.org/10.1016/j.isci.2019.09.016 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Aslam, Yusuf Williamson, James Romashova, Veronika Elder, Elizabeth Krishna, Benjamin Wills, Mark Lehner, Paul Sinclair, John Poole, Emma Human Cytomegalovirus Upregulates Expression of HCLS1 Resulting in Increased Cell Motility and Transendothelial Migration during Latency |
title | Human Cytomegalovirus Upregulates Expression of HCLS1 Resulting in Increased Cell Motility and Transendothelial Migration during Latency |
title_full | Human Cytomegalovirus Upregulates Expression of HCLS1 Resulting in Increased Cell Motility and Transendothelial Migration during Latency |
title_fullStr | Human Cytomegalovirus Upregulates Expression of HCLS1 Resulting in Increased Cell Motility and Transendothelial Migration during Latency |
title_full_unstemmed | Human Cytomegalovirus Upregulates Expression of HCLS1 Resulting in Increased Cell Motility and Transendothelial Migration during Latency |
title_short | Human Cytomegalovirus Upregulates Expression of HCLS1 Resulting in Increased Cell Motility and Transendothelial Migration during Latency |
title_sort | human cytomegalovirus upregulates expression of hcls1 resulting in increased cell motility and transendothelial migration during latency |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6817630/ https://www.ncbi.nlm.nih.gov/pubmed/31569051 http://dx.doi.org/10.1016/j.isci.2019.09.016 |
work_keys_str_mv | AT aslamyusuf humancytomegalovirusupregulatesexpressionofhcls1resultinginincreasedcellmotilityandtransendothelialmigrationduringlatency AT williamsonjames humancytomegalovirusupregulatesexpressionofhcls1resultinginincreasedcellmotilityandtransendothelialmigrationduringlatency AT romashovaveronika humancytomegalovirusupregulatesexpressionofhcls1resultinginincreasedcellmotilityandtransendothelialmigrationduringlatency AT elderelizabeth humancytomegalovirusupregulatesexpressionofhcls1resultinginincreasedcellmotilityandtransendothelialmigrationduringlatency AT krishnabenjamin humancytomegalovirusupregulatesexpressionofhcls1resultinginincreasedcellmotilityandtransendothelialmigrationduringlatency AT willsmark humancytomegalovirusupregulatesexpressionofhcls1resultinginincreasedcellmotilityandtransendothelialmigrationduringlatency AT lehnerpaul humancytomegalovirusupregulatesexpressionofhcls1resultinginincreasedcellmotilityandtransendothelialmigrationduringlatency AT sinclairjohn humancytomegalovirusupregulatesexpressionofhcls1resultinginincreasedcellmotilityandtransendothelialmigrationduringlatency AT pooleemma humancytomegalovirusupregulatesexpressionofhcls1resultinginincreasedcellmotilityandtransendothelialmigrationduringlatency |