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Synapsins are expressed at neuronal and non-neuronal locations in Octopus vulgaris

Synapsins are a family of phosphoproteins fundamental to the regulation of neurotransmitter release. They are typically neuron-specific, although recent evidence pointed to their expression in non-neuronal cells where they play a role in exocytosis and vesicle trafficking. In this work, we character...

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Autores principales: Maiole, Federica, Tedeschi, Giulia, Candiani, Simona, Maragliano, Luca, Benfenati, Fabio, Zullo, Letizia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6817820/
https://www.ncbi.nlm.nih.gov/pubmed/31659209
http://dx.doi.org/10.1038/s41598-019-51899-y
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author Maiole, Federica
Tedeschi, Giulia
Candiani, Simona
Maragliano, Luca
Benfenati, Fabio
Zullo, Letizia
author_facet Maiole, Federica
Tedeschi, Giulia
Candiani, Simona
Maragliano, Luca
Benfenati, Fabio
Zullo, Letizia
author_sort Maiole, Federica
collection PubMed
description Synapsins are a family of phosphoproteins fundamental to the regulation of neurotransmitter release. They are typically neuron-specific, although recent evidence pointed to their expression in non-neuronal cells where they play a role in exocytosis and vesicle trafficking. In this work, we characterized synapsin transcripts in the invertebrate mollusk Octopus vulgaris and present evidence of their expression not only in the brain but also in male and female reproductive organs. We identified three synapsin isoforms phylogenetically correlated to that of other invertebrates and with a modular structure characteristic of mammalian synapsins with a central, highly conserved C domain, important for the protein functions, and less conserved A, B and E domains. Our molecular modeling analysis further provided a solid background for predicting synapsin functional binding to ATP, actin filaments and secretory vesicles. Interestingly, we found that synapsin expression in ovary and testis increased during sexual maturation in cells with a known secretory role, potentially matching the occurrence of a secretion process. This might indicate that its secretory role has evolved across animals according to cell activity in spite of cell identity. We believe that this study may yield insights into the convergent evolution of ubiquitously expressed proteins between vertebrates and invertebrates.
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spelling pubmed-68178202019-11-01 Synapsins are expressed at neuronal and non-neuronal locations in Octopus vulgaris Maiole, Federica Tedeschi, Giulia Candiani, Simona Maragliano, Luca Benfenati, Fabio Zullo, Letizia Sci Rep Article Synapsins are a family of phosphoproteins fundamental to the regulation of neurotransmitter release. They are typically neuron-specific, although recent evidence pointed to their expression in non-neuronal cells where they play a role in exocytosis and vesicle trafficking. In this work, we characterized synapsin transcripts in the invertebrate mollusk Octopus vulgaris and present evidence of their expression not only in the brain but also in male and female reproductive organs. We identified three synapsin isoforms phylogenetically correlated to that of other invertebrates and with a modular structure characteristic of mammalian synapsins with a central, highly conserved C domain, important for the protein functions, and less conserved A, B and E domains. Our molecular modeling analysis further provided a solid background for predicting synapsin functional binding to ATP, actin filaments and secretory vesicles. Interestingly, we found that synapsin expression in ovary and testis increased during sexual maturation in cells with a known secretory role, potentially matching the occurrence of a secretion process. This might indicate that its secretory role has evolved across animals according to cell activity in spite of cell identity. We believe that this study may yield insights into the convergent evolution of ubiquitously expressed proteins between vertebrates and invertebrates. Nature Publishing Group UK 2019-10-28 /pmc/articles/PMC6817820/ /pubmed/31659209 http://dx.doi.org/10.1038/s41598-019-51899-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Maiole, Federica
Tedeschi, Giulia
Candiani, Simona
Maragliano, Luca
Benfenati, Fabio
Zullo, Letizia
Synapsins are expressed at neuronal and non-neuronal locations in Octopus vulgaris
title Synapsins are expressed at neuronal and non-neuronal locations in Octopus vulgaris
title_full Synapsins are expressed at neuronal and non-neuronal locations in Octopus vulgaris
title_fullStr Synapsins are expressed at neuronal and non-neuronal locations in Octopus vulgaris
title_full_unstemmed Synapsins are expressed at neuronal and non-neuronal locations in Octopus vulgaris
title_short Synapsins are expressed at neuronal and non-neuronal locations in Octopus vulgaris
title_sort synapsins are expressed at neuronal and non-neuronal locations in octopus vulgaris
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6817820/
https://www.ncbi.nlm.nih.gov/pubmed/31659209
http://dx.doi.org/10.1038/s41598-019-51899-y
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