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Proteins and microRNAs are differentially expressed in tear fluid from patients with Alzheimer’s disease

Alzheimer’s disease (AD) is characterized by a progressive loss of neurons and cognitive functions. Therefore, early diagnosis of AD is critical. The development of practical and non-invasive diagnostic tests for AD remains, however, an unmet need. In the present proof-of-concept study we investigat...

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Autores principales: Kenny, Aidan, Jiménez-Mateos, Eva M., Zea-Sevilla, María Ascensión, Rábano, Alberto, Gili-Manzanaro, Pablo, Prehn, Jochen H. M., Henshall, David C., Ávila, Jesús, Engel, Tobias, Hernández, Félix
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6817868/
https://www.ncbi.nlm.nih.gov/pubmed/31659197
http://dx.doi.org/10.1038/s41598-019-51837-y
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author Kenny, Aidan
Jiménez-Mateos, Eva M.
Zea-Sevilla, María Ascensión
Rábano, Alberto
Gili-Manzanaro, Pablo
Prehn, Jochen H. M.
Henshall, David C.
Ávila, Jesús
Engel, Tobias
Hernández, Félix
author_facet Kenny, Aidan
Jiménez-Mateos, Eva M.
Zea-Sevilla, María Ascensión
Rábano, Alberto
Gili-Manzanaro, Pablo
Prehn, Jochen H. M.
Henshall, David C.
Ávila, Jesús
Engel, Tobias
Hernández, Félix
author_sort Kenny, Aidan
collection PubMed
description Alzheimer’s disease (AD) is characterized by a progressive loss of neurons and cognitive functions. Therefore, early diagnosis of AD is critical. The development of practical and non-invasive diagnostic tests for AD remains, however, an unmet need. In the present proof-of-concept study we investigated tear fluid as a novel source of disease-specific protein and microRNA-based biomarkers for AD development using samples from patients with mild cognitive impairment (MCI) and AD. Tear protein content was evaluated via liquid chromatography-mass spectrometry and microRNA content was profiled using a genome-wide high-throughput PCR-based platform. These complementary approaches identified enrichment of specific proteins and microRNAs in tear fluid of AD patients. In particular, we identified elongation initiation factor 4E (eIF4E) as a unique protein present only in AD samples. Total microRNA abundance was found to be higher in tears from AD patients. Among individual microRNAs, microRNA-200b-5p was identified as a potential biomarker for AD with elevated levels present in AD tear fluid samples compared to controls. Our study suggests that tears may be a useful novel source of biomarkers for AD and that the identification and verification of biomarkers within tears may allow for the development of a non-invasive and cost-effective diagnostic test for AD.
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spelling pubmed-68178682019-11-01 Proteins and microRNAs are differentially expressed in tear fluid from patients with Alzheimer’s disease Kenny, Aidan Jiménez-Mateos, Eva M. Zea-Sevilla, María Ascensión Rábano, Alberto Gili-Manzanaro, Pablo Prehn, Jochen H. M. Henshall, David C. Ávila, Jesús Engel, Tobias Hernández, Félix Sci Rep Article Alzheimer’s disease (AD) is characterized by a progressive loss of neurons and cognitive functions. Therefore, early diagnosis of AD is critical. The development of practical and non-invasive diagnostic tests for AD remains, however, an unmet need. In the present proof-of-concept study we investigated tear fluid as a novel source of disease-specific protein and microRNA-based biomarkers for AD development using samples from patients with mild cognitive impairment (MCI) and AD. Tear protein content was evaluated via liquid chromatography-mass spectrometry and microRNA content was profiled using a genome-wide high-throughput PCR-based platform. These complementary approaches identified enrichment of specific proteins and microRNAs in tear fluid of AD patients. In particular, we identified elongation initiation factor 4E (eIF4E) as a unique protein present only in AD samples. Total microRNA abundance was found to be higher in tears from AD patients. Among individual microRNAs, microRNA-200b-5p was identified as a potential biomarker for AD with elevated levels present in AD tear fluid samples compared to controls. Our study suggests that tears may be a useful novel source of biomarkers for AD and that the identification and verification of biomarkers within tears may allow for the development of a non-invasive and cost-effective diagnostic test for AD. Nature Publishing Group UK 2019-10-28 /pmc/articles/PMC6817868/ /pubmed/31659197 http://dx.doi.org/10.1038/s41598-019-51837-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kenny, Aidan
Jiménez-Mateos, Eva M.
Zea-Sevilla, María Ascensión
Rábano, Alberto
Gili-Manzanaro, Pablo
Prehn, Jochen H. M.
Henshall, David C.
Ávila, Jesús
Engel, Tobias
Hernández, Félix
Proteins and microRNAs are differentially expressed in tear fluid from patients with Alzheimer’s disease
title Proteins and microRNAs are differentially expressed in tear fluid from patients with Alzheimer’s disease
title_full Proteins and microRNAs are differentially expressed in tear fluid from patients with Alzheimer’s disease
title_fullStr Proteins and microRNAs are differentially expressed in tear fluid from patients with Alzheimer’s disease
title_full_unstemmed Proteins and microRNAs are differentially expressed in tear fluid from patients with Alzheimer’s disease
title_short Proteins and microRNAs are differentially expressed in tear fluid from patients with Alzheimer’s disease
title_sort proteins and micrornas are differentially expressed in tear fluid from patients with alzheimer’s disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6817868/
https://www.ncbi.nlm.nih.gov/pubmed/31659197
http://dx.doi.org/10.1038/s41598-019-51837-y
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