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Glioblastoma-Associated Microglia Reprogramming Is Mediated by Functional Transfer of Extracellular miR-21
Gliomas are primary, diffusely infiltrating brain tumors. Microglia are innate immune cells in the CNS and make up a substantial portion of the tumor mass. Glioma cells shape their microenvironment, communicating with and reprogramming surrounding cells, resulting in enhanced angiogenesis, immune su...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6817978/ https://www.ncbi.nlm.nih.gov/pubmed/31533034 http://dx.doi.org/10.1016/j.celrep.2019.08.036 |
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author | Abels, Erik R. Maas, Sybren L.N. Nieland, Lisa Wei, Zhiyun Cheah, Pike See Tai, Eric Kolsteeg, Christy-Joy Dusoswa, Sophie A. Ting, David T. Hickman, Suzanne El Khoury, Joseph Krichevsky, Anna M. Broekman, Marike L.D. Breakefield, Xandra O. |
author_facet | Abels, Erik R. Maas, Sybren L.N. Nieland, Lisa Wei, Zhiyun Cheah, Pike See Tai, Eric Kolsteeg, Christy-Joy Dusoswa, Sophie A. Ting, David T. Hickman, Suzanne El Khoury, Joseph Krichevsky, Anna M. Broekman, Marike L.D. Breakefield, Xandra O. |
author_sort | Abels, Erik R. |
collection | PubMed |
description | Gliomas are primary, diffusely infiltrating brain tumors. Microglia are innate immune cells in the CNS and make up a substantial portion of the tumor mass. Glioma cells shape their microenvironment, communicating with and reprogramming surrounding cells, resulting in enhanced angiogenesis, immune suppression, and remodeling of the extracellular matrix. Glioma cells communicate with microglia, in part by releasing extracellular vesicles (EVs). Mouse glioma cells stably expressing a palmitoylated GFP to label EVs were implanted intracranially into syngeneic miR-21-null mice. Here, we demonstrate functional delivery of miR-21, regulating specific downstream mRNA targets in microglia after uptake of tumor-derived EVs. These findings attest to EV-dependent microRNA delivery as studied in an in vivo-based model and provide insight into the reprograming of microglial cells by tumor cells to create a favorable microenvironment for cancer progression. |
format | Online Article Text |
id | pubmed-6817978 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
record_format | MEDLINE/PubMed |
spelling | pubmed-68179782019-10-29 Glioblastoma-Associated Microglia Reprogramming Is Mediated by Functional Transfer of Extracellular miR-21 Abels, Erik R. Maas, Sybren L.N. Nieland, Lisa Wei, Zhiyun Cheah, Pike See Tai, Eric Kolsteeg, Christy-Joy Dusoswa, Sophie A. Ting, David T. Hickman, Suzanne El Khoury, Joseph Krichevsky, Anna M. Broekman, Marike L.D. Breakefield, Xandra O. Cell Rep Article Gliomas are primary, diffusely infiltrating brain tumors. Microglia are innate immune cells in the CNS and make up a substantial portion of the tumor mass. Glioma cells shape their microenvironment, communicating with and reprogramming surrounding cells, resulting in enhanced angiogenesis, immune suppression, and remodeling of the extracellular matrix. Glioma cells communicate with microglia, in part by releasing extracellular vesicles (EVs). Mouse glioma cells stably expressing a palmitoylated GFP to label EVs were implanted intracranially into syngeneic miR-21-null mice. Here, we demonstrate functional delivery of miR-21, regulating specific downstream mRNA targets in microglia after uptake of tumor-derived EVs. These findings attest to EV-dependent microRNA delivery as studied in an in vivo-based model and provide insight into the reprograming of microglial cells by tumor cells to create a favorable microenvironment for cancer progression. 2019-09-17 /pmc/articles/PMC6817978/ /pubmed/31533034 http://dx.doi.org/10.1016/j.celrep.2019.08.036 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Abels, Erik R. Maas, Sybren L.N. Nieland, Lisa Wei, Zhiyun Cheah, Pike See Tai, Eric Kolsteeg, Christy-Joy Dusoswa, Sophie A. Ting, David T. Hickman, Suzanne El Khoury, Joseph Krichevsky, Anna M. Broekman, Marike L.D. Breakefield, Xandra O. Glioblastoma-Associated Microglia Reprogramming Is Mediated by Functional Transfer of Extracellular miR-21 |
title | Glioblastoma-Associated Microglia Reprogramming Is Mediated by Functional Transfer of Extracellular miR-21 |
title_full | Glioblastoma-Associated Microglia Reprogramming Is Mediated by Functional Transfer of Extracellular miR-21 |
title_fullStr | Glioblastoma-Associated Microglia Reprogramming Is Mediated by Functional Transfer of Extracellular miR-21 |
title_full_unstemmed | Glioblastoma-Associated Microglia Reprogramming Is Mediated by Functional Transfer of Extracellular miR-21 |
title_short | Glioblastoma-Associated Microglia Reprogramming Is Mediated by Functional Transfer of Extracellular miR-21 |
title_sort | glioblastoma-associated microglia reprogramming is mediated by functional transfer of extracellular mir-21 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6817978/ https://www.ncbi.nlm.nih.gov/pubmed/31533034 http://dx.doi.org/10.1016/j.celrep.2019.08.036 |
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