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Diet‐induced obesity decreases rate‐dependent depression in the Hoffmann’s reflex in adult mice
Obesity is associated with balance and motor control deficits. We have recently shown that Group Ia muscle spindle afferents, the sensory arm of the muscle stretch reflex, are less responsive in mice fed a high‐fat diet. Here we test the hypothesis that reflex excitability to sensory information fro...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6818099/ https://www.ncbi.nlm.nih.gov/pubmed/31660698 http://dx.doi.org/10.14814/phy2.14271 |
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author | Nguyen, Gerard L. Putnam, Shea Haile, Mulatwa Raza, Zahra Bremer, Martina Wilkinson, Katherine A. |
author_facet | Nguyen, Gerard L. Putnam, Shea Haile, Mulatwa Raza, Zahra Bremer, Martina Wilkinson, Katherine A. |
author_sort | Nguyen, Gerard L. |
collection | PubMed |
description | Obesity is associated with balance and motor control deficits. We have recently shown that Group Ia muscle spindle afferents, the sensory arm of the muscle stretch reflex, are less responsive in mice fed a high‐fat diet. Here we test the hypothesis that reflex excitability to sensory information from Group Ia muscle spindle afferents is altered in a mouse model of diet‐induced obesity. We measured the anesthetized Hoffmann’s or H‐reflex, the electrical analog of the muscle stretch reflex. Adult mice of both sexes were fed a control diet (CD; 10% kcal from fat) or a high‐fat diet (HFD; 60% kcal from fat) for 5, 10, or 15 weeks. We used three quantitative measures of H‐reflex excitability: (1) H‐reflex latency; (2) the percentage of motor neurons recruited from electrical stimulation of Group Ia muscle spindle afferents (H(max)/M(max)); and (3) rate‐dependent depression (RDD), the decrease in H‐reflex amplitude to high frequency stimulation (20 stimuli at 5 Hz). A HFD did not significantly alter H latency (P = 0.16) or H(max)/M(max) ratios (P = 0.06), but RDD was significantly lower in HFD compared to CD groups (P < 0.001). Interestingly, HFD males exhibited decreased RDD compared to controls only after 5 and 10 weeks of feeding, but females showed progressive decreases in RDD that were only significant at 10 and 15 weeks on the HFD. These results suggest that high‐fat feeding increases H‐reflex excitability. Future studies are needed to determine whether these changes alter muscle stretch reflex strength and/or balance and to determine the underlying mechanism(s). |
format | Online Article Text |
id | pubmed-6818099 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-68180992019-11-04 Diet‐induced obesity decreases rate‐dependent depression in the Hoffmann’s reflex in adult mice Nguyen, Gerard L. Putnam, Shea Haile, Mulatwa Raza, Zahra Bremer, Martina Wilkinson, Katherine A. Physiol Rep Original Research Obesity is associated with balance and motor control deficits. We have recently shown that Group Ia muscle spindle afferents, the sensory arm of the muscle stretch reflex, are less responsive in mice fed a high‐fat diet. Here we test the hypothesis that reflex excitability to sensory information from Group Ia muscle spindle afferents is altered in a mouse model of diet‐induced obesity. We measured the anesthetized Hoffmann’s or H‐reflex, the electrical analog of the muscle stretch reflex. Adult mice of both sexes were fed a control diet (CD; 10% kcal from fat) or a high‐fat diet (HFD; 60% kcal from fat) for 5, 10, or 15 weeks. We used three quantitative measures of H‐reflex excitability: (1) H‐reflex latency; (2) the percentage of motor neurons recruited from electrical stimulation of Group Ia muscle spindle afferents (H(max)/M(max)); and (3) rate‐dependent depression (RDD), the decrease in H‐reflex amplitude to high frequency stimulation (20 stimuli at 5 Hz). A HFD did not significantly alter H latency (P = 0.16) or H(max)/M(max) ratios (P = 0.06), but RDD was significantly lower in HFD compared to CD groups (P < 0.001). Interestingly, HFD males exhibited decreased RDD compared to controls only after 5 and 10 weeks of feeding, but females showed progressive decreases in RDD that were only significant at 10 and 15 weeks on the HFD. These results suggest that high‐fat feeding increases H‐reflex excitability. Future studies are needed to determine whether these changes alter muscle stretch reflex strength and/or balance and to determine the underlying mechanism(s). John Wiley and Sons Inc. 2019-10-29 /pmc/articles/PMC6818099/ /pubmed/31660698 http://dx.doi.org/10.14814/phy2.14271 Text en © 2019 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Nguyen, Gerard L. Putnam, Shea Haile, Mulatwa Raza, Zahra Bremer, Martina Wilkinson, Katherine A. Diet‐induced obesity decreases rate‐dependent depression in the Hoffmann’s reflex in adult mice |
title | Diet‐induced obesity decreases rate‐dependent depression in the Hoffmann’s reflex in adult mice |
title_full | Diet‐induced obesity decreases rate‐dependent depression in the Hoffmann’s reflex in adult mice |
title_fullStr | Diet‐induced obesity decreases rate‐dependent depression in the Hoffmann’s reflex in adult mice |
title_full_unstemmed | Diet‐induced obesity decreases rate‐dependent depression in the Hoffmann’s reflex in adult mice |
title_short | Diet‐induced obesity decreases rate‐dependent depression in the Hoffmann’s reflex in adult mice |
title_sort | diet‐induced obesity decreases rate‐dependent depression in the hoffmann’s reflex in adult mice |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6818099/ https://www.ncbi.nlm.nih.gov/pubmed/31660698 http://dx.doi.org/10.14814/phy2.14271 |
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