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Molecular surveillance of antimicrobial resistance and transmission pattern of Mycobacterium leprae in Chinese leprosy patients

Reports on antimicrobial resistance (AMR) of Mycobacterium leprae, relationship with bacteriological index (BI), and transmission in China are limited. We investigated the emergence of AMR mutations, the relationship between BI and AMR in complete, moderate and lack of BI decline cases, and molecula...

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Autores principales: Chokkakula, Santosh, Chen, Zhiming, Wang, Le, Jiang, Haiqin, Chen, Yanqing, Shi, Ying, Zhang, Wenyue, Gao, Wei, Yang, Jun, Li, Jinlan, Li, Xiong, Shui, Tiejun, He, Jun, Shen, Limei, Liu, Jie, Wang, De, Wang, Hao, Chen, Huan, Kuang, Yanfei, Li, Bin, Chen, Ziyi, Wu, Aiping, Yu, Meiwen, Yan, Liangbin, Suryadevara, Naveen Chandra, Vissa, Varalakshmi, Liu, Weida, Wang, Hongsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6818117/
https://www.ncbi.nlm.nih.gov/pubmed/31621517
http://dx.doi.org/10.1080/22221751.2019.1677177
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author Chokkakula, Santosh
Chen, Zhiming
Wang, Le
Jiang, Haiqin
Chen, Yanqing
Shi, Ying
Zhang, Wenyue
Gao, Wei
Yang, Jun
Li, Jinlan
Li, Xiong
Shui, Tiejun
He, Jun
Shen, Limei
Liu, Jie
Wang, De
Wang, Hao
Chen, Huan
Kuang, Yanfei
Li, Bin
Chen, Ziyi
Wu, Aiping
Yu, Meiwen
Yan, Liangbin
Suryadevara, Naveen Chandra
Vissa, Varalakshmi
Liu, Weida
Wang, Hongsheng
author_facet Chokkakula, Santosh
Chen, Zhiming
Wang, Le
Jiang, Haiqin
Chen, Yanqing
Shi, Ying
Zhang, Wenyue
Gao, Wei
Yang, Jun
Li, Jinlan
Li, Xiong
Shui, Tiejun
He, Jun
Shen, Limei
Liu, Jie
Wang, De
Wang, Hao
Chen, Huan
Kuang, Yanfei
Li, Bin
Chen, Ziyi
Wu, Aiping
Yu, Meiwen
Yan, Liangbin
Suryadevara, Naveen Chandra
Vissa, Varalakshmi
Liu, Weida
Wang, Hongsheng
author_sort Chokkakula, Santosh
collection PubMed
description Reports on antimicrobial resistance (AMR) of Mycobacterium leprae, relationship with bacteriological index (BI), and transmission in China are limited. We investigated the emergence of AMR mutations, the relationship between BI and AMR in complete, moderate and lack of BI decline cases, and molecular epidemiological features of AMR cases by enrolling 290 leprosy cases from four endemic provinces. Seven (2.41%), one (0.34%), five (1.72%), one (0.34%), and one (0.34%) strains had single mutations in folP1, rpoC, gyrA, gyrB, and 23S rRNA, respectively. Double mutations in folP1 and gyrA, rpoB and gyrA, and gyrA and 23S rRNA were observed in one (0.34%) strain each. Mutated strains occurred in three out of 81 (95% CI−0.005-0.079, p = 0.083) cases with complete BI decline, in seven out of 103 (95% CI 0.018-0.117, p = 0.008) cases with moderate BI decline, and in four out of 34 (95% CI 0.003-0.231, p = 0.044) cases with lack of BI decline. Most of these mutated strains were geographically separated and diverged genotypically. AMR mutations may not be the main cause of the lack of BI decline. The low transmission of AMR strains at the county level indicates an ongoing transmission at close contact levels.
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spelling pubmed-68181172019-11-05 Molecular surveillance of antimicrobial resistance and transmission pattern of Mycobacterium leprae in Chinese leprosy patients Chokkakula, Santosh Chen, Zhiming Wang, Le Jiang, Haiqin Chen, Yanqing Shi, Ying Zhang, Wenyue Gao, Wei Yang, Jun Li, Jinlan Li, Xiong Shui, Tiejun He, Jun Shen, Limei Liu, Jie Wang, De Wang, Hao Chen, Huan Kuang, Yanfei Li, Bin Chen, Ziyi Wu, Aiping Yu, Meiwen Yan, Liangbin Suryadevara, Naveen Chandra Vissa, Varalakshmi Liu, Weida Wang, Hongsheng Emerg Microbes Infect Original Articles Reports on antimicrobial resistance (AMR) of Mycobacterium leprae, relationship with bacteriological index (BI), and transmission in China are limited. We investigated the emergence of AMR mutations, the relationship between BI and AMR in complete, moderate and lack of BI decline cases, and molecular epidemiological features of AMR cases by enrolling 290 leprosy cases from four endemic provinces. Seven (2.41%), one (0.34%), five (1.72%), one (0.34%), and one (0.34%) strains had single mutations in folP1, rpoC, gyrA, gyrB, and 23S rRNA, respectively. Double mutations in folP1 and gyrA, rpoB and gyrA, and gyrA and 23S rRNA were observed in one (0.34%) strain each. Mutated strains occurred in three out of 81 (95% CI−0.005-0.079, p = 0.083) cases with complete BI decline, in seven out of 103 (95% CI 0.018-0.117, p = 0.008) cases with moderate BI decline, and in four out of 34 (95% CI 0.003-0.231, p = 0.044) cases with lack of BI decline. Most of these mutated strains were geographically separated and diverged genotypically. AMR mutations may not be the main cause of the lack of BI decline. The low transmission of AMR strains at the county level indicates an ongoing transmission at close contact levels. Taylor & Francis 2019-10-17 /pmc/articles/PMC6818117/ /pubmed/31621517 http://dx.doi.org/10.1080/22221751.2019.1677177 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Chokkakula, Santosh
Chen, Zhiming
Wang, Le
Jiang, Haiqin
Chen, Yanqing
Shi, Ying
Zhang, Wenyue
Gao, Wei
Yang, Jun
Li, Jinlan
Li, Xiong
Shui, Tiejun
He, Jun
Shen, Limei
Liu, Jie
Wang, De
Wang, Hao
Chen, Huan
Kuang, Yanfei
Li, Bin
Chen, Ziyi
Wu, Aiping
Yu, Meiwen
Yan, Liangbin
Suryadevara, Naveen Chandra
Vissa, Varalakshmi
Liu, Weida
Wang, Hongsheng
Molecular surveillance of antimicrobial resistance and transmission pattern of Mycobacterium leprae in Chinese leprosy patients
title Molecular surveillance of antimicrobial resistance and transmission pattern of Mycobacterium leprae in Chinese leprosy patients
title_full Molecular surveillance of antimicrobial resistance and transmission pattern of Mycobacterium leprae in Chinese leprosy patients
title_fullStr Molecular surveillance of antimicrobial resistance and transmission pattern of Mycobacterium leprae in Chinese leprosy patients
title_full_unstemmed Molecular surveillance of antimicrobial resistance and transmission pattern of Mycobacterium leprae in Chinese leprosy patients
title_short Molecular surveillance of antimicrobial resistance and transmission pattern of Mycobacterium leprae in Chinese leprosy patients
title_sort molecular surveillance of antimicrobial resistance and transmission pattern of mycobacterium leprae in chinese leprosy patients
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6818117/
https://www.ncbi.nlm.nih.gov/pubmed/31621517
http://dx.doi.org/10.1080/22221751.2019.1677177
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