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Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging
Monoamine neurotransmitters are released by specialized neurons regulating behavioral, motor, and cognitive functions. Although the localization of monoaminergic neurons in the brain is well known, the distribution and kinetics of monoamines remain unclear. Here, we generated a murine brain atlas of...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6818351/ https://www.ncbi.nlm.nih.gov/pubmed/31614319 http://dx.doi.org/10.1016/j.isci.2019.09.036 |
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author | Sugiyama, Eiji Guerrini, Matteo M. Honda, Kurara Hattori, Yuko Abe, Manabu Källback, Patrik Andrén, Per E. Tanaka, Kenji F. Setou, Mitsutoshi Fagarasan, Sidonia Suematsu, Makoto Sugiura, Yuki |
author_facet | Sugiyama, Eiji Guerrini, Matteo M. Honda, Kurara Hattori, Yuko Abe, Manabu Källback, Patrik Andrén, Per E. Tanaka, Kenji F. Setou, Mitsutoshi Fagarasan, Sidonia Suematsu, Makoto Sugiura, Yuki |
author_sort | Sugiyama, Eiji |
collection | PubMed |
description | Monoamine neurotransmitters are released by specialized neurons regulating behavioral, motor, and cognitive functions. Although the localization of monoaminergic neurons in the brain is well known, the distribution and kinetics of monoamines remain unclear. Here, we generated a murine brain atlas of serotonin (5-HT), dopamine (DA), and norepinephrine (NE) levels using mass spectrometry imaging (MSI). We found several nuclei rich in both 5-HT and a catecholamine (DA or NE) and identified the paraventricular nucleus of the thalamus (PVT), where 5-HT and NE are co-localized. The analysis of 5-HT fluctuations in response to acute tryptophan depletion and infusion of isotope-labeled tryptophan in vivo revealed a close kinetic association between the raphe nuclei, PVT, and amygdala but not the other nuclei. Our findings imply the existence of a highly dynamic 5-HT-mediated raphe to PVT pathway that likely plays a role in the brain monoamine system. |
format | Online Article Text |
id | pubmed-6818351 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-68183512019-11-01 Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging Sugiyama, Eiji Guerrini, Matteo M. Honda, Kurara Hattori, Yuko Abe, Manabu Källback, Patrik Andrén, Per E. Tanaka, Kenji F. Setou, Mitsutoshi Fagarasan, Sidonia Suematsu, Makoto Sugiura, Yuki iScience Article Monoamine neurotransmitters are released by specialized neurons regulating behavioral, motor, and cognitive functions. Although the localization of monoaminergic neurons in the brain is well known, the distribution and kinetics of monoamines remain unclear. Here, we generated a murine brain atlas of serotonin (5-HT), dopamine (DA), and norepinephrine (NE) levels using mass spectrometry imaging (MSI). We found several nuclei rich in both 5-HT and a catecholamine (DA or NE) and identified the paraventricular nucleus of the thalamus (PVT), where 5-HT and NE are co-localized. The analysis of 5-HT fluctuations in response to acute tryptophan depletion and infusion of isotope-labeled tryptophan in vivo revealed a close kinetic association between the raphe nuclei, PVT, and amygdala but not the other nuclei. Our findings imply the existence of a highly dynamic 5-HT-mediated raphe to PVT pathway that likely plays a role in the brain monoamine system. Elsevier 2019-09-27 /pmc/articles/PMC6818351/ /pubmed/31614319 http://dx.doi.org/10.1016/j.isci.2019.09.036 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sugiyama, Eiji Guerrini, Matteo M. Honda, Kurara Hattori, Yuko Abe, Manabu Källback, Patrik Andrén, Per E. Tanaka, Kenji F. Setou, Mitsutoshi Fagarasan, Sidonia Suematsu, Makoto Sugiura, Yuki Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging |
title | Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging |
title_full | Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging |
title_fullStr | Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging |
title_full_unstemmed | Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging |
title_short | Detection of a High-Turnover Serotonin Circuit in the Mouse Brain Using Mass Spectrometry Imaging |
title_sort | detection of a high-turnover serotonin circuit in the mouse brain using mass spectrometry imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6818351/ https://www.ncbi.nlm.nih.gov/pubmed/31614319 http://dx.doi.org/10.1016/j.isci.2019.09.036 |
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