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QTL mapping for aluminum tolerance in RIL population of soybean (Glycine max L.) by RAD sequencing

Aluminum (Al(3+)) toxicity is a typical abiotic stress that severely limits crop production in acidic soils. In this study, an RIL (recombinant inbred line, F(12)) population derived from the cross of Zhonghuang 24 (ZH 24) and Huaxia 3 (HX 3) (160 lines) was tested using hydroponic cultivation. Rela...

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Autores principales: Wang, Xinxin, Cheng, Yanbo, Yang, Ce, Yang, Cunyi, Mu, Yinghui, Xia, Qiuju, Ma, Qibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6818782/
https://www.ncbi.nlm.nih.gov/pubmed/31661499
http://dx.doi.org/10.1371/journal.pone.0223674
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author Wang, Xinxin
Cheng, Yanbo
Yang, Ce
Yang, Cunyi
Mu, Yinghui
Xia, Qiuju
Ma, Qibin
author_facet Wang, Xinxin
Cheng, Yanbo
Yang, Ce
Yang, Cunyi
Mu, Yinghui
Xia, Qiuju
Ma, Qibin
author_sort Wang, Xinxin
collection PubMed
description Aluminum (Al(3+)) toxicity is a typical abiotic stress that severely limits crop production in acidic soils. In this study, an RIL (recombinant inbred line, F(12)) population derived from the cross of Zhonghuang 24 (ZH 24) and Huaxia 3 (HX 3) (160 lines) was tested using hydroponic cultivation. Relative root elongation (RRE) and apical Al(3+) content (AAC) were evaluated for each line, and a significant negative correlation was detected between the two indicators. Based on a high-density genetic linkage map, the phenotypic data were used to identify quantitative trait loci (QTLs) associated with these traits. With composite interval mapping (CIM) of the linkage map, five QTLs that explained 39.65% of RRE and AAC variation were detected on chromosomes (Chrs) Gm04, Gm16, Gm17 and Gm19. Two new QTLs, qRRE_04 and qAAC_04, were located on the same region of bin93-bin94 on Chr Gm04, which explained 7.09% and 8.98% phenotypic variation, respectively. Furthermore, the results of the expression analysis of candidate genes in the five genetic regions of the QTLs showed that six genes (Glyma.04g218700, Glyma.04g212800, Glyma.04g213300, Glyma.04g217400, Glyma.04g216100 and Glyma.04g220600) exhibited significant differential expression between the Al(3+) treatment and the control of two parents. The results of qRT-PCR analysis indicated that Glyma.04g218700 was upregulated by Al(3+) treatment with the hundreds-fold increased expression level and may be a candidate gene with potential roles in the response to aluminum stress. Therefore, our efforts will enable future functional analysis of candidate genes and will contribute to the strategies for improvement of aluminum tolerance in soybean.
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spelling pubmed-68187822019-11-01 QTL mapping for aluminum tolerance in RIL population of soybean (Glycine max L.) by RAD sequencing Wang, Xinxin Cheng, Yanbo Yang, Ce Yang, Cunyi Mu, Yinghui Xia, Qiuju Ma, Qibin PLoS One Research Article Aluminum (Al(3+)) toxicity is a typical abiotic stress that severely limits crop production in acidic soils. In this study, an RIL (recombinant inbred line, F(12)) population derived from the cross of Zhonghuang 24 (ZH 24) and Huaxia 3 (HX 3) (160 lines) was tested using hydroponic cultivation. Relative root elongation (RRE) and apical Al(3+) content (AAC) were evaluated for each line, and a significant negative correlation was detected between the two indicators. Based on a high-density genetic linkage map, the phenotypic data were used to identify quantitative trait loci (QTLs) associated with these traits. With composite interval mapping (CIM) of the linkage map, five QTLs that explained 39.65% of RRE and AAC variation were detected on chromosomes (Chrs) Gm04, Gm16, Gm17 and Gm19. Two new QTLs, qRRE_04 and qAAC_04, were located on the same region of bin93-bin94 on Chr Gm04, which explained 7.09% and 8.98% phenotypic variation, respectively. Furthermore, the results of the expression analysis of candidate genes in the five genetic regions of the QTLs showed that six genes (Glyma.04g218700, Glyma.04g212800, Glyma.04g213300, Glyma.04g217400, Glyma.04g216100 and Glyma.04g220600) exhibited significant differential expression between the Al(3+) treatment and the control of two parents. The results of qRT-PCR analysis indicated that Glyma.04g218700 was upregulated by Al(3+) treatment with the hundreds-fold increased expression level and may be a candidate gene with potential roles in the response to aluminum stress. Therefore, our efforts will enable future functional analysis of candidate genes and will contribute to the strategies for improvement of aluminum tolerance in soybean. Public Library of Science 2019-10-29 /pmc/articles/PMC6818782/ /pubmed/31661499 http://dx.doi.org/10.1371/journal.pone.0223674 Text en © 2019 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wang, Xinxin
Cheng, Yanbo
Yang, Ce
Yang, Cunyi
Mu, Yinghui
Xia, Qiuju
Ma, Qibin
QTL mapping for aluminum tolerance in RIL population of soybean (Glycine max L.) by RAD sequencing
title QTL mapping for aluminum tolerance in RIL population of soybean (Glycine max L.) by RAD sequencing
title_full QTL mapping for aluminum tolerance in RIL population of soybean (Glycine max L.) by RAD sequencing
title_fullStr QTL mapping for aluminum tolerance in RIL population of soybean (Glycine max L.) by RAD sequencing
title_full_unstemmed QTL mapping for aluminum tolerance in RIL population of soybean (Glycine max L.) by RAD sequencing
title_short QTL mapping for aluminum tolerance in RIL population of soybean (Glycine max L.) by RAD sequencing
title_sort qtl mapping for aluminum tolerance in ril population of soybean (glycine max l.) by rad sequencing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6818782/
https://www.ncbi.nlm.nih.gov/pubmed/31661499
http://dx.doi.org/10.1371/journal.pone.0223674
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