Catalytides derived from the Box A region in the ANA/BTG3 protein cleave amyloid-β fragment peptide

We have recently reported about shorter proteolytic peptides termed Catalytide as general name. JAL-TA9 (YKGSGFRMI), a fragment peptide derived from Box A region of Tob1 protein, is the first Catalytide and cleaves Aβ42 and its fragment peptides. Herein, we demonstrate the enzymatic properties of AN...

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Detalles Bibliográficos
Autores principales: Hatakawa, Yusuke, Nakamura, Rina, Konishi, Motomi, Sakane, Toshiyasu, Saito, Motoaki, Akizawa, Toshifumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6819762/
https://www.ncbi.nlm.nih.gov/pubmed/31687556
http://dx.doi.org/10.1016/j.heliyon.2019.e02454
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author Hatakawa, Yusuke
Nakamura, Rina
Konishi, Motomi
Sakane, Toshiyasu
Saito, Motoaki
Akizawa, Toshifumi
author_facet Hatakawa, Yusuke
Nakamura, Rina
Konishi, Motomi
Sakane, Toshiyasu
Saito, Motoaki
Akizawa, Toshifumi
author_sort Hatakawa, Yusuke
collection PubMed
description We have recently reported about shorter proteolytic peptides termed Catalytide as general name. JAL-TA9 (YKGSGFRMI), a fragment peptide derived from Box A region of Tob1 protein, is the first Catalytide and cleaves Aβ42 and its fragment peptides. Herein, we demonstrate the enzymatic properties of ANA-TA9 corresponding region to JAL-TA9 in ANA/BTG3 protein. ANA-TA9 showed the auto-proteolytic activity and cleaved 3 kinds of synthetic fragment peptides derived from Aβ42, especially on the central region of Aβ42 with a serine protease like activity. Interestingly, 2 kinds of components, ANA-SA5 (SKGQA) and ANA-YA4 (YRMI), also showed similar proteolytic activity. These results indicate that ANA-TA9 is composed of two different Catalytides.
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spelling pubmed-68197622019-11-04 Catalytides derived from the Box A region in the ANA/BTG3 protein cleave amyloid-β fragment peptide Hatakawa, Yusuke Nakamura, Rina Konishi, Motomi Sakane, Toshiyasu Saito, Motoaki Akizawa, Toshifumi Heliyon Article We have recently reported about shorter proteolytic peptides termed Catalytide as general name. JAL-TA9 (YKGSGFRMI), a fragment peptide derived from Box A region of Tob1 protein, is the first Catalytide and cleaves Aβ42 and its fragment peptides. Herein, we demonstrate the enzymatic properties of ANA-TA9 corresponding region to JAL-TA9 in ANA/BTG3 protein. ANA-TA9 showed the auto-proteolytic activity and cleaved 3 kinds of synthetic fragment peptides derived from Aβ42, especially on the central region of Aβ42 with a serine protease like activity. Interestingly, 2 kinds of components, ANA-SA5 (SKGQA) and ANA-YA4 (YRMI), also showed similar proteolytic activity. These results indicate that ANA-TA9 is composed of two different Catalytides. Elsevier 2019-09-24 /pmc/articles/PMC6819762/ /pubmed/31687556 http://dx.doi.org/10.1016/j.heliyon.2019.e02454 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hatakawa, Yusuke
Nakamura, Rina
Konishi, Motomi
Sakane, Toshiyasu
Saito, Motoaki
Akizawa, Toshifumi
Catalytides derived from the Box A region in the ANA/BTG3 protein cleave amyloid-β fragment peptide
title Catalytides derived from the Box A region in the ANA/BTG3 protein cleave amyloid-β fragment peptide
title_full Catalytides derived from the Box A region in the ANA/BTG3 protein cleave amyloid-β fragment peptide
title_fullStr Catalytides derived from the Box A region in the ANA/BTG3 protein cleave amyloid-β fragment peptide
title_full_unstemmed Catalytides derived from the Box A region in the ANA/BTG3 protein cleave amyloid-β fragment peptide
title_short Catalytides derived from the Box A region in the ANA/BTG3 protein cleave amyloid-β fragment peptide
title_sort catalytides derived from the box a region in the ana/btg3 protein cleave amyloid-β fragment peptide
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6819762/
https://www.ncbi.nlm.nih.gov/pubmed/31687556
http://dx.doi.org/10.1016/j.heliyon.2019.e02454
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