Identification and characterization of oligomeric proanthocyanidins with significant anti-cancer activity in adzuki beans (Vigna angularis)

The aim of the present study was to characterize and evaluate the anti-cancer activity of proanthocyanidin-enriched fractions from adzuki beans. For this purpose, we concentrated proanthocyanidins from adzuki beans (Vigna angularis) into five fractions using Amberlite XAD-1180N, Toyopearl HW40F, and...

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Autores principales: Kawahara, Sei-ichi, Ishihara, Chisato, Matsumoto, Kiriko, Senga, Shogo, Kawaguchi, Koichiro, Yamamoto, Ayaka, Suwannachot, Jutalak, Hamauzu, Yasunori, Makabe, Hidefumi, Fujii, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6820087/
https://www.ncbi.nlm.nih.gov/pubmed/31687492
http://dx.doi.org/10.1016/j.heliyon.2019.e02610
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author Kawahara, Sei-ichi
Ishihara, Chisato
Matsumoto, Kiriko
Senga, Shogo
Kawaguchi, Koichiro
Yamamoto, Ayaka
Suwannachot, Jutalak
Hamauzu, Yasunori
Makabe, Hidefumi
Fujii, Hiroshi
author_facet Kawahara, Sei-ichi
Ishihara, Chisato
Matsumoto, Kiriko
Senga, Shogo
Kawaguchi, Koichiro
Yamamoto, Ayaka
Suwannachot, Jutalak
Hamauzu, Yasunori
Makabe, Hidefumi
Fujii, Hiroshi
author_sort Kawahara, Sei-ichi
collection PubMed
description The aim of the present study was to characterize and evaluate the anti-cancer activity of proanthocyanidin-enriched fractions from adzuki beans. For this purpose, we concentrated proanthocyanidins from adzuki beans (Vigna angularis) into five fractions using Amberlite XAD-1180N, Toyopearl HW40F, and Sepacore C-18 reverse-phase flash column chromatography. Proanthocyanidin-enriched fractions were characterized as (epi)catechin hexamer, heptamer, and octamer, epigallocatechin-(epi)catechin pentamer, and epigallocatechin-(epi)catechin hexamer using electrospray ionization time-of-flight mass spectrometry and thiolytic degradation. These fractions showed significant anti-cancer activity against the human PC-3 prostate cancer cell line. They also significantly suppressed the expression of the fatty acid-binding protein 5 gene, which plays critical roles in cell growth and metastasis in prostate cancer.
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spelling pubmed-68200872019-11-04 Identification and characterization of oligomeric proanthocyanidins with significant anti-cancer activity in adzuki beans (Vigna angularis) Kawahara, Sei-ichi Ishihara, Chisato Matsumoto, Kiriko Senga, Shogo Kawaguchi, Koichiro Yamamoto, Ayaka Suwannachot, Jutalak Hamauzu, Yasunori Makabe, Hidefumi Fujii, Hiroshi Heliyon Article The aim of the present study was to characterize and evaluate the anti-cancer activity of proanthocyanidin-enriched fractions from adzuki beans. For this purpose, we concentrated proanthocyanidins from adzuki beans (Vigna angularis) into five fractions using Amberlite XAD-1180N, Toyopearl HW40F, and Sepacore C-18 reverse-phase flash column chromatography. Proanthocyanidin-enriched fractions were characterized as (epi)catechin hexamer, heptamer, and octamer, epigallocatechin-(epi)catechin pentamer, and epigallocatechin-(epi)catechin hexamer using electrospray ionization time-of-flight mass spectrometry and thiolytic degradation. These fractions showed significant anti-cancer activity against the human PC-3 prostate cancer cell line. They also significantly suppressed the expression of the fatty acid-binding protein 5 gene, which plays critical roles in cell growth and metastasis in prostate cancer. Elsevier 2019-10-23 /pmc/articles/PMC6820087/ /pubmed/31687492 http://dx.doi.org/10.1016/j.heliyon.2019.e02610 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kawahara, Sei-ichi
Ishihara, Chisato
Matsumoto, Kiriko
Senga, Shogo
Kawaguchi, Koichiro
Yamamoto, Ayaka
Suwannachot, Jutalak
Hamauzu, Yasunori
Makabe, Hidefumi
Fujii, Hiroshi
Identification and characterization of oligomeric proanthocyanidins with significant anti-cancer activity in adzuki beans (Vigna angularis)
title Identification and characterization of oligomeric proanthocyanidins with significant anti-cancer activity in adzuki beans (Vigna angularis)
title_full Identification and characterization of oligomeric proanthocyanidins with significant anti-cancer activity in adzuki beans (Vigna angularis)
title_fullStr Identification and characterization of oligomeric proanthocyanidins with significant anti-cancer activity in adzuki beans (Vigna angularis)
title_full_unstemmed Identification and characterization of oligomeric proanthocyanidins with significant anti-cancer activity in adzuki beans (Vigna angularis)
title_short Identification and characterization of oligomeric proanthocyanidins with significant anti-cancer activity in adzuki beans (Vigna angularis)
title_sort identification and characterization of oligomeric proanthocyanidins with significant anti-cancer activity in adzuki beans (vigna angularis)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6820087/
https://www.ncbi.nlm.nih.gov/pubmed/31687492
http://dx.doi.org/10.1016/j.heliyon.2019.e02610
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