Cargando…

Significance of transient receptor potential vanilloid 4 and aquaporin 5 co-expression in the rat uterus at term

AIMS: Aquaporins (AQPs) are channel proteins that facilitate the rapid passive movement of water. In our studies it was proved that the decreased AQP5 expression is followed by the increase of uterine contractility. The transient receptor potential vanilloid 4 (TRPV4) is a calcium channel, which is...

Descripción completa

Detalles Bibliográficos
Autores principales: Ducza, Eszter, Csányi, Adrienn, Szőke, Éva, Pohóczky, Krisztina, Hajagos-Tóth, Judit, Kothencz, Anna, Tiszai, Zita, Gáspár, Róbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6820280/
https://www.ncbi.nlm.nih.gov/pubmed/31687520
http://dx.doi.org/10.1016/j.heliyon.2019.e02697
_version_ 1783463904775503872
author Ducza, Eszter
Csányi, Adrienn
Szőke, Éva
Pohóczky, Krisztina
Hajagos-Tóth, Judit
Kothencz, Anna
Tiszai, Zita
Gáspár, Róbert
author_facet Ducza, Eszter
Csányi, Adrienn
Szőke, Éva
Pohóczky, Krisztina
Hajagos-Tóth, Judit
Kothencz, Anna
Tiszai, Zita
Gáspár, Róbert
author_sort Ducza, Eszter
collection PubMed
description AIMS: Aquaporins (AQPs) are channel proteins that facilitate the rapid passive movement of water. In our studies it was proved that the decreased AQP5 expression is followed by the increase of uterine contractility. The transient receptor potential vanilloid 4 (TRPV4) is a calcium channel, which is activated in response to osmotic changes. Our aim was to determine the possible role of AQP5 in this osmotic regulation of TRPV4, thus in pregnant uterine contraction. MAIN METHODS: We used RT-PCR and Western blot techniques for the detection of the TRPV4 expression during pregnancy in rat uterus. The localization of AQP5 and TRPV4 was determined by immunohistochemical studies. The role of TRPV4 in uterus contraction was investigated in an isolated organ bath system. In vitro uterus contractions were stimulated with KCl and its effect was investigated with the selective TRPV4 agonist (RN1747) and antagonist (RN1734). KEY FINDINGS: The TRPV4 expression continuously increased from day 18 to the last day of pregnancy. The co-expression of TRPV4 and AQP5 in the myometrium and endometrium was determined in the late pregnant uterus. The TRPV4 antagonist and agonist significantly decreased and increased uterine contraction, respectively, especially on the last day of pregnancy. SIGNIFICANCE: We presume the decreased AQP5 expression triggers hypertonic stress, which activates TRPV4 and increases uterus contraction on the day of labor. Based on these findings, we suppose the TRPV4 effect on uterus contraction is AQP5 control, which could be a new target in preterm birth therapy.
format Online
Article
Text
id pubmed-6820280
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-68202802019-11-04 Significance of transient receptor potential vanilloid 4 and aquaporin 5 co-expression in the rat uterus at term Ducza, Eszter Csányi, Adrienn Szőke, Éva Pohóczky, Krisztina Hajagos-Tóth, Judit Kothencz, Anna Tiszai, Zita Gáspár, Róbert Heliyon Article AIMS: Aquaporins (AQPs) are channel proteins that facilitate the rapid passive movement of water. In our studies it was proved that the decreased AQP5 expression is followed by the increase of uterine contractility. The transient receptor potential vanilloid 4 (TRPV4) is a calcium channel, which is activated in response to osmotic changes. Our aim was to determine the possible role of AQP5 in this osmotic regulation of TRPV4, thus in pregnant uterine contraction. MAIN METHODS: We used RT-PCR and Western blot techniques for the detection of the TRPV4 expression during pregnancy in rat uterus. The localization of AQP5 and TRPV4 was determined by immunohistochemical studies. The role of TRPV4 in uterus contraction was investigated in an isolated organ bath system. In vitro uterus contractions were stimulated with KCl and its effect was investigated with the selective TRPV4 agonist (RN1747) and antagonist (RN1734). KEY FINDINGS: The TRPV4 expression continuously increased from day 18 to the last day of pregnancy. The co-expression of TRPV4 and AQP5 in the myometrium and endometrium was determined in the late pregnant uterus. The TRPV4 antagonist and agonist significantly decreased and increased uterine contraction, respectively, especially on the last day of pregnancy. SIGNIFICANCE: We presume the decreased AQP5 expression triggers hypertonic stress, which activates TRPV4 and increases uterus contraction on the day of labor. Based on these findings, we suppose the TRPV4 effect on uterus contraction is AQP5 control, which could be a new target in preterm birth therapy. Elsevier 2019-10-22 /pmc/articles/PMC6820280/ /pubmed/31687520 http://dx.doi.org/10.1016/j.heliyon.2019.e02697 Text en © 2019 The Authors. Published by Elsevier Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Ducza, Eszter
Csányi, Adrienn
Szőke, Éva
Pohóczky, Krisztina
Hajagos-Tóth, Judit
Kothencz, Anna
Tiszai, Zita
Gáspár, Róbert
Significance of transient receptor potential vanilloid 4 and aquaporin 5 co-expression in the rat uterus at term
title Significance of transient receptor potential vanilloid 4 and aquaporin 5 co-expression in the rat uterus at term
title_full Significance of transient receptor potential vanilloid 4 and aquaporin 5 co-expression in the rat uterus at term
title_fullStr Significance of transient receptor potential vanilloid 4 and aquaporin 5 co-expression in the rat uterus at term
title_full_unstemmed Significance of transient receptor potential vanilloid 4 and aquaporin 5 co-expression in the rat uterus at term
title_short Significance of transient receptor potential vanilloid 4 and aquaporin 5 co-expression in the rat uterus at term
title_sort significance of transient receptor potential vanilloid 4 and aquaporin 5 co-expression in the rat uterus at term
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6820280/
https://www.ncbi.nlm.nih.gov/pubmed/31687520
http://dx.doi.org/10.1016/j.heliyon.2019.e02697
work_keys_str_mv AT duczaeszter significanceoftransientreceptorpotentialvanilloid4andaquaporin5coexpressionintheratuterusatterm
AT csanyiadrienn significanceoftransientreceptorpotentialvanilloid4andaquaporin5coexpressionintheratuterusatterm
AT szokeeva significanceoftransientreceptorpotentialvanilloid4andaquaporin5coexpressionintheratuterusatterm
AT pohoczkykrisztina significanceoftransientreceptorpotentialvanilloid4andaquaporin5coexpressionintheratuterusatterm
AT hajagostothjudit significanceoftransientreceptorpotentialvanilloid4andaquaporin5coexpressionintheratuterusatterm
AT kothenczanna significanceoftransientreceptorpotentialvanilloid4andaquaporin5coexpressionintheratuterusatterm
AT tiszaizita significanceoftransientreceptorpotentialvanilloid4andaquaporin5coexpressionintheratuterusatterm
AT gasparrobert significanceoftransientreceptorpotentialvanilloid4andaquaporin5coexpressionintheratuterusatterm